LOCUS       sequence-428836-        8904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a single point mutant of melanoma 
            differentiation-associated protein 5 lacking its 2 CARD domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8904)
  TITLE     MDA5 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET50_G495R∆2CARD
FEATURES             Location/Qualifiers
     source          1..8904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(19..66)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     62..80
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(161..205)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(206..223)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(2477..2500)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(2510..2527)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2543..4027)
                     /codon_start=1
                     /gene="nusA"
                     /product="transcription elongation protein (N utilization 
                     substance protein A)"
                     /label=NusA
                     /note="highly soluble in E. coli"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDV
                     RVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDR
                     ITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVIL
                     REDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIE
                     IKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPA
                     QFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMT
                     VDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLD
                     EPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLED
                     LAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     CDS             complement(4034..4051)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             4071..4093
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    4108..4132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4132..4151)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4133..4151)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4239..4258)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        4464..4541
                     /gene="lacI"
                     /label=lacI promoter
     CDS             4542..5624
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4561..4580)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             6196..6387
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6381..6403)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6489..6628
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6544..6563
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6643..6660)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6814..7402)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6894..6913)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7513..7532)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7524..8339
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             7524..8339
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7597..7616)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(8431..8886)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8568..8589)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8780..8799
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tatagttcct cctttcagca aaaaacccct caagacccgt ttagaggccc caaggggtta
       61 tgctagttat tgctcagcgg tggcagcagc caactcagct tcctttcggg ctttgtttag
      121 cagcctaggt taattaagcc tcgagagcag cagaagtaga gctgtccatg tgctggcgtt
      181 cgaatttagc agcagcggtt tctttactac cgcgtggcac cagagcgagc tctgcggccg
      241 caagcttgtc gacctaatcc tcatcactaa ataaacagca ttctgaatag tcaagattgg
      301 gaaatgtgat aggtaattct acccactttt tgtattgttt ctttgttgaa ttatttttga
      361 aaaccactac aaaattcctt attttgagac aaggcaaatc taagcctttg tgcaccatca
      421 ttgttcccca agcctggcca catttgcaga tgatttcacc atttatttga tagtcggcac
      481 acttcttttg cagtgttttg ttttctctta caatgtaaag ttccttgaat tctggggtca
      541 tattgacgtg atgcattttc tcaattacat ggatatcttc cccagaacag gctagcacac
      601 tgcagttttt gcaaaggaaa gttattagtg atgggttatt cttgtaatgc ttggcaatat
      661 ttctcttggt tttcattttc ttttccatta tactttgcat ctgtaattcc aaaatcttat
      721 gagcatactc ctctggtttc atattttgaa cacaatgtat agctttatac atcatcttct
      781 ctcggaaatc attaactgtc tcacgttcga taactcctga accactgtga gcaaccagga
      841 cgtaggtgct ctcatcagct ctggctcgac cacgggcctg gaccatggct atttcattgg
      901 tgacgagacc ataacggata acaatgttac attctttaat atccagacct tcttctgcca
      961 ctgtggtagc gataagcaga tttatttttc cagtgcgaaa tttactaatg acttcttttt
     1021 gttcattctg tgtcatgggt ttgaactcac tgctgtgtcc agctccaatc agatggtggg
     1081 ctttgactcc tacttcagca aatttttcat tttcagtaat ccactgggaa agcgcatatg
     1141 cactctgtcg tgtttttgta aagattattc ctcgtgctga ttcctcagtc ctagtatatt
     1201 gctccattat ggtatttctt aatttggtca gcttttcatt ttcatattct gggttttcag
     1261 ccagcctttt caacatttta ttgttttcaa aaaataaagt catgagaaat ctatctgttt
     1321 catccagttt caaaggtttc tttaaatcat cctcatcttc atcaccatca caatactcat
     1381 catcaccacc ctcatcacta tcatcttcta tgactgcaaa cttcttatct ttctcttcat
     1441 tatagaaagt ttcaagatga gtatacgcat ctatcattcg aattgtgtca ttaatttgta
     1501 gggcctcatt gtacttcctc aaatgttctg cacaaacacg ttctttgcga tttccttctt
     1561 ttgcagcttt tttttccatt tgaatggccc attgttcata gggttgagtt ccaaaatctg
     1621 acattggact catttgacaa taagtttgaa tccttgtcat tatttctaga agtttctctt
     1681 taaatggatc ttctctggtt gcatctgcaa tggcaaactt cttgcatggc tcctgtattt
     1741 ggtttttcag ttgatcaagg ttttctttaa cagttttaat agtaaatgca tcaagattgg
     1801 cacatagttt taaaatgtgt tcttcagctt tggcttgctt cgtggccctt ccaacaccag
     1861 gtgaagctgt tagtcccagt atctgaggaa ggggaatcac tggtttgttt tctttcttga
     1921 gtctattgtt tttcaacttc tgcatcaaat aatgcctcat gatgttatta tacactgctt
     1981 ctttgttggt gtgatgacat tcatcaatga taatgaggga aaagtctgac aattgaacac
     2041 cagcatcttc tccattttcc aagtttaaga gggagttttc aaggatttga gctgtactga
     2101 taataatatc acaggacttg acaacttctg gaaatgatat tttcagttgg gtatcaccac
     2161 ttaatccaat aacacgatac catttcttca aaaatggttg gaactccttg cggaagagct
     2221 gttcaactag cagtacctta ttgacaagaa ctataacttt tccaggctca gatgcttttt
     2281 tcttcttgtc taagtgatcc ttggcaatgt aaacagccac tctggttttt ccactccctg
     2341 tagggaggca gatgatgata ttcttccctt ccaaggctgg ctgggcaact tccatttggt
     2401 aaggcctgag ctggagttct ggctccgggg atgctcttgc tgccacattc tcttcatctg
     2461 aatcacttcc catcccgggt ccctgaaaga ggacttcaag agccgcggag tgatggtgat
     2521 ggtgatgacc agaaccacta gtcgcttcgt caccgaacca gcaaatatta cgggcagcca
     2581 taatcagtgc tccggctttt tcgtcggtca acccttcgat atcagccaga tcatcaatgc
     2641 cctgttcggc gagatcttcc agcgtacaaa cgccacgggc ggccagtttg aatgccaaat
     2701 cacgatctac cccttcaagg ttcagcagat cgtcagccgg tttgttatca ccgaggcttt
     2761 cttcctgggc ctgtgcaatg gtggccagtg catttttagc acgctcgcgc agtgcttcaa
     2821 cggtcggctc atcaaggcct tcgatttcca acagctcttt catcggcaca taggccaatt
     2881 cttccagcgt cgagaagcct tcttctacca gaacagtcgc gaagtcttcg tcgatgtcga
     2941 gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta gcttgcaggt
     3001 cgtcaacggt catcacgttg agttcccaac cgctcagttg cgaagccaga cgcacgttct
     3061 gaccgttacg gccaatcgcc tgcgccagat taccggcttc aacggcgatg tccatggtgt
     3121 gtttatcttc atccaccacg atagaagcaa cgtctgccgg tgccattgcg ttaatcacga
     3181 actgcgccgg gttatcatcc cacaggacga tatcgatacg ctcgccaccc agttcagtag
     3241 acaccgcctg aacacgcgcg ccacgcatac ctacgcaagc acctaccgga tcgatacgtt
     3301 tatcgttggt tttcaccgcg attttcgcac gagaacccgg atcgcgagcc gctgctttaa
     3361 tttcaatcac ttcttcgccg atttctggca cttcaatacg gaacagttcg atcagcattt
     3421 ccggcttgga acgagtgacg aacagttgcg cgccacgcgc ttccgggcga acggaataga
     3481 gcacgccacg aacgcggtcg ccagggcgga agttttcacg cggcagcata tcttcgcgca
     3541 ggatcacggc ttcagcgttg ttgcccagat ccagagagat gttgtcgcgg tttacttttt
     3601 tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc accatcgcac
     3661 gttcggcttc acgcactttc tgcacgataa cctgttttgc cgtctgggta gtgatacggt
     3721 caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt
     3781 cataacgtgc ggcttcaagg gtgatttcct tggtcggctg ggtgacttca tcaacaacta
     3841 accagcgacg gaaagtgtca aaatcaccgc ttttgcgatc gatctgtacg cggacgtcga
     3901 tctcttgttc atattttttc tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa
     3961 tcttctcgcg aggtagcgcc ttttcattgg atacggcttc aactacagcc aaaatttctt
     4021 tgttcatact agaatggtga tggtggtgat ggctgctgcc catatgtata tctccttctt
     4081 aaagttaaac aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt
     4141 gagtcgtatt aatttcgcgg gatcgagatc gatctcgatc ctctacgccg gacgcatcgt
     4201 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     4261 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     4321 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     4381 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     4441 taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg
     4501 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat
     4561 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
     4621 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
     4681 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
     4741 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
     4801 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
     4861 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
     4921 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
     4981 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
     5041 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
     5101 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
     5161 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
     5221 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg
     5281 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
     5341 acatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa
     5401 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     5461 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     5521 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     5581 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     5641 gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac
     5701 ccagtcagct ccttccggtg ggcgcggggc atgactagca tgatcgtgct cctgtcgttg
     5761 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
     5821 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
     5881 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
     5941 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
     6001 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
     6061 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
     6121 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct
     6181 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
     6241 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca
     6301 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
     6361 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     6421 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     6481 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     6541 atgcggcatc agagcagatt gtactgagag tgcaccattg cggtgtgaaa taccgcacag
     6601 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     6661 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6721 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6781 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6841 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6901 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6961 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7021 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7081 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7141 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7201 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7261 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7321 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7381 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7441 gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg tctgcttaca
     7501 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctctaggc
     7561 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     7621 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt
     7681 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     7741 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     7801 atgcatggtt actcaccact gcgatccccg gcaaaacagc attccaggta ttagaagaat
     7861 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     7921 cgattcctgt ttgtaattgt ccttttaaca gtgatcgcgt atttcgtctc gctcaggcgc
     7981 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     8041 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     8101 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     8161 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     8221 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     8281 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaag
     8341 aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8401 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     8461 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     8521 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     8581 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     8641 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     8701 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     8761 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     8821 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     8881 gcgcgtccca ttcgccaatc cgga
//
