LOCUS       sequence-428965-        8679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET-based reporter for Ras activity, ER localized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Zhang JZ, Nguyen WH, Greenwood N, Rose JC, Ong SE, Maly DJ, Baker D
  TITLE     Computationally designed sensors detect endogenous Ras activity and 
            signaling effectors at subcellular resolution.
  JOURNAL   Nat Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w.
  PUBMED    38273065
REFERENCE   2  (bases 1 to 8679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w."
COMMENT     Sequence Label: pcDNA3 ER-Ras-LOCKR-S
FEATURES             Location/Qualifiers
     source          1..8679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      897..906
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             993..1010
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1017..1046
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1056..1085
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1095..1124
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2031..2087
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2088..2111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2121..2144
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2154..2177
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4270..4287)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4276..4500
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4546..4974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4633..4652)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4843..4864
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4983..5003)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4988..5317
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5168..5303
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5230..5249
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5384..6178
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5438..5457)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6048..6067
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6352..6473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6389..6408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6443..6462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6522..6538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6522..6538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6535..6557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6546..6562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6570..6576,6577..6594,6595..6600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6615..6636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6753..6770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6924..7512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7004..7023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7683..8474,8475..8543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8306..8325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8544..8648)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcgcca
      901 ccatggatcc cgtggtggta ttgggcttgt gtctctcatg tctgcttctc ttgtcattgt
      961 ggaaacaatc ctacggaggt ggcgggtcta gccatcacca tcatcaccat tctagcgagc
     1021 agaaactcat ctctgaagag gatctcggtg gtagtgaaca aaagttgata tccgaggagg
     1081 atttgggtgg gagcgagcag aaacttatca gtgaagaaga tttggggggc tccatggatc
     1141 ctgacgaggc aaggaaagcc atagcgaggg ttaaaaggga aagcaaacga atagtagaag
     1201 acgcggaaag gcttagtcga gaagcagcgg cggcaagtga gaaatcctcc cgagaggcgg
     1261 agaggctctc ccgggaggcc gctgctgcgt ccgagaaaat ttcccgggag ggaggaacag
     1321 gggggtcagg agggactgga ggctcaatgg tatctaaagg agaagagctg tttactgggg
     1381 tcgtgcctat cctggtagag ctggacggtg acgttaatgg acataagttt agcgtaagtg
     1441 gggaaggcga aggcgatgct acttacggga agttgacttt gaaatttatc tgtaccactg
     1501 gaaagctgcc ggtaccctgg cctacgttgg ttaccacgtt gacctggggt gtccaatgct
     1561 tctcaagata cccagatcac atgaagcaac acgacttttt caaatctgcg atgcccgaag
     1621 gatatgttca agagagaacg atatttttta aagatgatgg taactataaa actagagccg
     1681 aggtcaagtt cgagggggac actctggtta accggatcga acttaaaggc attgacttta
     1741 aggaggatgg gaacatcctg ggccataaat tggagtacaa ctatatatca cataacgtct
     1801 atatcacggc ggacaagcaa aagaatggga taaaggctaa ctttaaaata cggcataaca
     1861 ttgaggatgg aagcgtgcag cttgcggacc attatcaaca aaatacgcct ataggagacg
     1921 gacccgtgct gctgcctgat aatcactatc tttccactca aagtgcgctc tctaaagacc
     1981 cgaatgagaa acgagaccat atggttctgt tggagttcgt aacggcagcg gcaaccaatt
     2041 tcagtctcct gaagcaggca ggtgatgtag aagagaatcc cggaccggat tataaagacg
     2101 atgatgacaa gggaggtagt gattacaagg atgatgacga taaaggaggt tccgattaca
     2161 aagatgatga cgacaagggc gggtctatga agggcgaaga acttttcaca ggcgtggtgc
     2221 cgatattggt ggaactcgat ggagacgtaa atgggcataa attctcagtt tccggggaag
     2281 gggaaggtga cgccacttat gggaagctta ctctcaaatt gctgtgcact acggggaagc
     2341 ttccagtgcc ttggcctaca cttgttacca cactggggta cggagtgcag tgtttcgccc
     2401 gctaccccga ccacatgaaa cagcatgatt tctttaaatc tgcgatgcca gaaggttatg
     2461 tgcaagagag aactattttt ttcaaggatg acggcaatta caaaactagg gcggaggtta
     2521 agttcgaggg tgacacactc gtgaatagga ttgagttgaa gggcatagac tttaaagaag
     2581 atgggaatat cttgggtcac aagcttgagt ataattataa ctcccataat gtctacatca
     2641 cagcagataa acagaaaaat ggaattaaag caaacttcaa gatacgacac aacatcgagg
     2701 acggtggagt tcagttggcg gaccactacc aacaaaatac acctatcggt gacgggccag
     2761 tccttctgcc cgacaaccac tacctctcct atcaaagcgc gcttttcaag gaccctaatg
     2821 agaagagaga ccatatggtt cttcttgaat ttctgacagc agcgggaggt accggcggtt
     2881 ccgggggtac gggaggcagt tcaaaggaag cagcgaagaa actccaggac ctgaatatag
     2941 agcttgcgcg gaaactcctc gaagcgagca cgaagcttca aagacttaac ataaggctgg
     3001 cagaagctct cttggaagcc atcgctagat tgcaggagct gaatctggag ctcgtatacc
     3061 tggctgttga gctgacagac cctaaacgga tacgggatga gatcaaggaa gtcaaagata
     3121 agagcaagga aattattcgc cgcgctgaaa aagaaataga tgatgctgcc aaggagtcca
     3181 aaaaaattct tgaggaagca cgaaaagcta ttcgagacgc cgccgaggag agtaggaaaa
     3241 ttctcgagga aggtagcggg agcgggtcag atgccctgga tgagttgcaa aagttgaatc
     3301 ttgaacttgc gaagcttctc cttaaagcaa ttgctgaaac tcaagacttg aatcttagag
     3361 ctgcaaaggc ctttctcgaa gcggccgcaa aattgcagga acttaatatt agggctgtgg
     3421 aacttctcgt aaagctcacc gacccggcaa ccatacgccg ggctctggag catgctaaga
     3481 gacggtcaaa ggagataatc gacgaagcgg aaagggctat tcgggcagct aagcgcgaga
     3541 gcgagcgaat tattgaagaa gcgcggcgac tcatagagaa ggccaaggag gagagtgaaa
     3601 ggataattcg agaaggttcc ggcagtggtg atcccgatat caaaaaattg caggatctca
     3661 acattgaact ggcaagagag ctgctccggg ctcatgcaca gcttcagcga cttaatttgg
     3721 agttgcttcg cgaactgctt cgagctttgg ctcaattgca agagcttaac ctcgacctct
     3781 tgaggttggc ttccgaattg actgacccag acgaggcgag gaaagcgatc gccgttacag
     3841 gctaccggct ctttgaagaa atactggaca gtgagcgatt gagtcgggag gccgcagcag
     3901 catctgaaaa gagcagccgg gaggctgaac gccttatacg agaagctgca gcggcttcta
     3961 gcaatactat aagggttttt ctccccaaca agcaacgcac cgtggtcaac gtgaggaacg
     4021 ggatgtcact gcacgattgc ctgatgaagg cgctgaaggt tcgaggtttg caacccgagt
     4081 gttgcgcggt gttcaggctt cttcacgagc ataaggggaa aaaagcacga ttggattgga
     4141 acactgatgc agccagtctc atcggcgagg aattgcaggt ggattttttg tgagaattct
     4201 gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc gtttaaaccc
     4261 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4321 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4381 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4441 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     4501 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     4561 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4621 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4681 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4741 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4801 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4861 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4921 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4981 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5041 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5101 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5161 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5221 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     5281 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     5341 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5401 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5461 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5521 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     5581 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5641 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5701 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5761 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5821 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5881 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     5941 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6001 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6061 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6121 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6181 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6241 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6301 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6361 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6421 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6481 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6541 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6601 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6661 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6721 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6901 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7081 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7201 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7261 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7321 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7381 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7441 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7501 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7561 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7621 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7681 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7741 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7801 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7861 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7921 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7981 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8041 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8101 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8161 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8221 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8281 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8341 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8401 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8461 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8521 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8581 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8641 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
