LOCUS       sequence-428966-        8697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET-based reporter for Ras activity, Golgi localized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Zhang JZ, Nguyen WH, Greenwood N, Rose JC, Ong SE, Maly DJ, Baker D
  TITLE     Computationally designed sensors detect endogenous Ras activity and 
            signaling effectors at subcellular resolution.
  JOURNAL   Nat Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w.
  PUBMED    38273065
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w."
COMMENT     Sequence Label: pcDNA3 Golgi-Ras-LOCKR-S
FEATURES             Location/Qualifiers
     source          1..8697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      897..906
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1011..1028
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1035..1064
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1074..1103
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1113..1142
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2049..2105
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2106..2129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2139..2162
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2172..2195
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4288..4305)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4294..4518
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4564..4992
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4651..4670)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4861..4882
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5001..5021)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5006..5335
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5186..5321
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5248..5267
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5402..6196
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5456..5475)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6066..6085
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6370..6491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6407..6426)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6461..6480
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6540..6556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6540..6556)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6553..6575)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6564..6580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6588..6594,6595..6612,6613..6618))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6633..6654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6771..6788)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6942..7530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7022..7041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7701..8492,8493..8561))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8324..8343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8562..8666)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcgcca
      901 ccatgggtaa cttgaaatca gttgcgcagg agccaggtcc accatgtggg ctggggctgg
      961 gtctcgggct tggactctgc ggtaagcagg gccccgcgac tgggtctagc catcaccatc
     1021 atcaccattc tagcgagcag aaactcatct ctgaagagga tctcggtggt agtgaacaaa
     1081 agttgatatc cgaggaggat ttgggtggga gcgagcagaa acttatcagt gaagaagatt
     1141 tggggggctc catggatcct gacgaggcaa ggaaagccat agcgagggtt aaaagggaaa
     1201 gcaaacgaat agtagaagac gcggaaaggc ttagtcgaga agcagcggcg gcaagtgaga
     1261 aatcctcccg agaggcggag aggctctccc gggaggccgc tgctgcgtcc gagaaaattt
     1321 cccgggaggg aggaacaggg gggtcaggag ggactggagg ctcaatggta tctaaaggag
     1381 aagagctgtt tactggggtc gtgcctatcc tggtagagct ggacggtgac gttaatggac
     1441 ataagtttag cgtaagtggg gaaggcgaag gcgatgctac ttacgggaag ttgactttga
     1501 aatttatctg taccactgga aagctgccgg taccctggcc tacgttggtt accacgttga
     1561 cctggggtgt ccaatgcttc tcaagatacc cagatcacat gaagcaacac gactttttca
     1621 aatctgcgat gcccgaagga tatgttcaag agagaacgat attttttaaa gatgatggta
     1681 actataaaac tagagccgag gtcaagttcg agggggacac tctggttaac cggatcgaac
     1741 ttaaaggcat tgactttaag gaggatggga acatcctggg ccataaattg gagtacaact
     1801 atatatcaca taacgtctat atcacggcgg acaagcaaaa gaatgggata aaggctaact
     1861 ttaaaatacg gcataacatt gaggatggaa gcgtgcagct tgcggaccat tatcaacaaa
     1921 atacgcctat aggagacgga cccgtgctgc tgcctgataa tcactatctt tccactcaaa
     1981 gtgcgctctc taaagacccg aatgagaaac gagaccatat ggttctgttg gagttcgtaa
     2041 cggcagcggc aaccaatttc agtctcctga agcaggcagg tgatgtagaa gagaatcccg
     2101 gaccggatta taaagacgat gatgacaagg gaggtagtga ttacaaggat gatgacgata
     2161 aaggaggttc cgattacaaa gatgatgacg acaagggcgg gtctatgaag ggcgaagaac
     2221 ttttcacagg cgtggtgccg atattggtgg aactcgatgg agacgtaaat gggcataaat
     2281 tctcagtttc cggggaaggg gaaggtgacg ccacttatgg gaagcttact ctcaaattgc
     2341 tgtgcactac ggggaagctt ccagtgcctt ggcctacact tgttaccaca ctggggtacg
     2401 gagtgcagtg tttcgcccgc taccccgacc acatgaaaca gcatgatttc tttaaatctg
     2461 cgatgccaga aggttatgtg caagagagaa ctattttttt caaggatgac ggcaattaca
     2521 aaactagggc ggaggttaag ttcgagggtg acacactcgt gaataggatt gagttgaagg
     2581 gcatagactt taaagaagat gggaatatct tgggtcacaa gcttgagtat aattataact
     2641 cccataatgt ctacatcaca gcagataaac agaaaaatgg aattaaagca aacttcaaga
     2701 tacgacacaa catcgaggac ggtggagttc agttggcgga ccactaccaa caaaatacac
     2761 ctatcggtga cgggccagtc cttctgcccg acaaccacta cctctcctat caaagcgcgc
     2821 ttttcaagga ccctaatgag aagagagacc atatggttct tcttgaattt ctgacagcag
     2881 cgggaggtac cggcggttcc gggggtacgg gaggcagttc aaaggaagca gcgaagaaac
     2941 tccaggacct gaatatagag cttgcgcgga aactcctcga agcgagcacg aagcttcaaa
     3001 gacttaacat aaggctggca gaagctctct tggaagccat cgctagattg caggagctga
     3061 atctggagct cgtatacctg gctgttgagc tgacagaccc taaacggata cgggatgaga
     3121 tcaaggaagt caaagataag agcaaggaaa ttattcgccg cgctgaaaaa gaaatagatg
     3181 atgctgccaa ggagtccaaa aaaattcttg aggaagcacg aaaagctatt cgagacgccg
     3241 ccgaggagag taggaaaatt ctcgaggaag gtagcgggag cgggtcagat gccctggatg
     3301 agttgcaaaa gttgaatctt gaacttgcga agcttctcct taaagcaatt gctgaaactc
     3361 aagacttgaa tcttagagct gcaaaggcct ttctcgaagc ggccgcaaaa ttgcaggaac
     3421 ttaatattag ggctgtggaa cttctcgtaa agctcaccga cccggcaacc atacgccggg
     3481 ctctggagca tgctaagaga cggtcaaagg agataatcga cgaagcggaa agggctattc
     3541 gggcagctaa gcgcgagagc gagcgaatta ttgaagaagc gcggcgactc atagagaagg
     3601 ccaaggagga gagtgaaagg ataattcgag aaggttccgg cagtggtgat cccgatatca
     3661 aaaaattgca ggatctcaac attgaactgg caagagagct gctccgggct catgcacagc
     3721 ttcagcgact taatttggag ttgcttcgcg aactgcttcg agctttggct caattgcaag
     3781 agcttaacct cgacctcttg aggttggctt ccgaattgac tgacccagac gaggcgagga
     3841 aagcgatcgc cgttacaggc taccggctct ttgaagaaat actggacagt gagcgattga
     3901 gtcgggaggc cgcagcagca tctgaaaaga gcagccggga ggctgaacgc cttatacgag
     3961 aagctgcagc ggcttctagc aatactataa gggtttttct ccccaacaag caacgcaccg
     4021 tggtcaacgt gaggaacggg atgtcactgc acgattgcct gatgaaggcg ctgaaggttc
     4081 gaggtttgca acccgagtgt tgcgcggtgt tcaggcttct tcacgagcat aaggggaaaa
     4141 aagcacgatt ggattggaac actgatgcag ccagtctcat cggcgaggaa ttgcaggtgg
     4201 attttttgtg agaattctgc agatatccat cacactggcg gccgctcgag catgcatcta
     4261 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     4321 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4381 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4441 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4501 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     4561 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4621 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4681 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4741 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4801 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4861 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4921 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4981 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5041 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5101 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5161 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5221 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     5281 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5341 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     5401 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5461 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5521 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5581 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5641 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5701 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5761 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5821 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5881 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5941 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     6001 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     6061 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     6121 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6181 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     6241 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6301 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6361 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6481 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     6541 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6601 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6661 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6721 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6781 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6841 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6901 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6961 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7021 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7081 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7141 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7201 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7261 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7321 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7381 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7441 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7501 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7561 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7621 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7681 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7741 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7801 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7861 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7921 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7981 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8041 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8101 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8161 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8221 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8281 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8341 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8401 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8461 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8521 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8581 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8641 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
