LOCUS       sequence-428986-        8631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET-based reporter for Ras activity, plasma membrane localized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Zhang JZ, Nguyen WH, Greenwood N, Rose JC, Ong SE, Maly DJ, Baker D
  TITLE     Computationally designed sensors detect endogenous Ras activity and 
            signaling effectors at subcellular resolution.
  JOURNAL   Nat Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w.
  PUBMED    38273065
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w."
COMMENT     Sequence Label: pcDNA3 PM-Ras-LOCKR-S
FEATURES             Location/Qualifiers
     source          1..8631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      897..906
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             945..962
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             969..998
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1008..1037
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1047..1076
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1983..2039
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2040..2063
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2073..2096
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2106..2129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4222..4239)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4228..4452
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4498..4926
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4585..4604)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4795..4816
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4935..4955)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4940..5269
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5120..5255
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5182..5201
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5336..6130
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5390..5409)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6000..6019
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6304..6425
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6341..6360)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6395..6414
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6474..6490)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6474..6490)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6487..6509)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6498..6514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6522..6528,6529..6546,6547..6552))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6567..6588
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6705..6722)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6876..7464)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6956..6975)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7635..8426,8427..8495))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8258..8277
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8496..8600)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcgcca
      901 ccatgggctg catcaagagc aagaggaagg acaaagggtc tagccatcac catcatcacc
      961 attctagcga gcagaaactc atctctgaag aggatctcgg tggtagtgaa caaaagttga
     1021 tatccgagga ggatttgggt gggagcgagc agaaacttat cagtgaagaa gatttggggg
     1081 gctccatgga tcctgacgag gcaaggaaag ccatagcgag ggttaaaagg gaaagcaaac
     1141 gaatagtaga agacgcggaa aggcttagtc gagaagcagc ggcggcaagt gagaaatcct
     1201 cccgagaggc ggagaggctc tcccgggagg ccgctgctgc gtccgagaaa atttcccggg
     1261 agggaggaac aggggggtca ggagggactg gaggctcaat ggtatctaaa ggagaagagc
     1321 tgtttactgg ggtcgtgcct atcctggtag agctggacgg tgacgttaat ggacataagt
     1381 ttagcgtaag tggggaaggc gaaggcgatg ctacttacgg gaagttgact ttgaaattta
     1441 tctgtaccac tggaaagctg ccggtaccct ggcctacgtt ggttaccacg ttgacctggg
     1501 gtgtccaatg cttctcaaga tacccagatc acatgaagca acacgacttt ttcaaatctg
     1561 cgatgcccga aggatatgtt caagagagaa cgatattttt taaagatgat ggtaactata
     1621 aaactagagc cgaggtcaag ttcgaggggg acactctggt taaccggatc gaacttaaag
     1681 gcattgactt taaggaggat gggaacatcc tgggccataa attggagtac aactatatat
     1741 cacataacgt ctatatcacg gcggacaagc aaaagaatgg gataaaggct aactttaaaa
     1801 tacggcataa cattgaggat ggaagcgtgc agcttgcgga ccattatcaa caaaatacgc
     1861 ctataggaga cggacccgtg ctgctgcctg ataatcacta tctttccact caaagtgcgc
     1921 tctctaaaga cccgaatgag aaacgagacc atatggttct gttggagttc gtaacggcag
     1981 cggcaaccaa tttcagtctc ctgaagcagg caggtgatgt agaagagaat cccggaccgg
     2041 attataaaga cgatgatgac aagggaggta gtgattacaa ggatgatgac gataaaggag
     2101 gttccgatta caaagatgat gacgacaagg gcgggtctat gaagggcgaa gaacttttca
     2161 caggcgtggt gccgatattg gtggaactcg atggagacgt aaatgggcat aaattctcag
     2221 tttccgggga aggggaaggt gacgccactt atgggaagct tactctcaaa ttgctgtgca
     2281 ctacggggaa gcttccagtg ccttggccta cacttgttac cacactgggg tacggagtgc
     2341 agtgtttcgc ccgctacccc gaccacatga aacagcatga tttctttaaa tctgcgatgc
     2401 cagaaggtta tgtgcaagag agaactattt ttttcaagga tgacggcaat tacaaaacta
     2461 gggcggaggt taagttcgag ggtgacacac tcgtgaatag gattgagttg aagggcatag
     2521 actttaaaga agatgggaat atcttgggtc acaagcttga gtataattat aactcccata
     2581 atgtctacat cacagcagat aaacagaaaa atggaattaa agcaaacttc aagatacgac
     2641 acaacatcga ggacggtgga gttcagttgg cggaccacta ccaacaaaat acacctatcg
     2701 gtgacgggcc agtccttctg cccgacaacc actacctctc ctatcaaagc gcgcttttca
     2761 aggaccctaa tgagaagaga gaccatatgg ttcttcttga atttctgaca gcagcgggag
     2821 gtaccggcgg ttccgggggt acgggaggca gttcaaagga agcagcgaag aaactccagg
     2881 acctgaatat agagcttgcg cggaaactcc tcgaagcgag cacgaagctt caaagactta
     2941 acataaggct ggcagaagct ctcttggaag ccatcgctag attgcaggag ctgaatctgg
     3001 agctcgtata cctggctgtt gagctgacag accctaaacg gatacgggat gagatcaagg
     3061 aagtcaaaga taagagcaag gaaattattc gccgcgctga aaaagaaata gatgatgctg
     3121 ccaaggagtc caaaaaaatt cttgaggaag cacgaaaagc tattcgagac gccgccgagg
     3181 agagtaggaa aattctcgag gaaggtagcg ggagcgggtc agatgccctg gatgagttgc
     3241 aaaagttgaa tcttgaactt gcgaagcttc tccttaaagc aattgctgaa actcaagact
     3301 tgaatcttag agctgcaaag gcctttctcg aagcggccgc aaaattgcag gaacttaata
     3361 ttagggctgt ggaacttctc gtaaagctca ccgacccggc aaccatacgc cgggctctgg
     3421 agcatgctaa gagacggtca aaggagataa tcgacgaagc ggaaagggct attcgggcag
     3481 ctaagcgcga gagcgagcga attattgaag aagcgcggcg actcatagag aaggccaagg
     3541 aggagagtga aaggataatt cgagaaggtt ccggcagtgg tgatcccgat atcaaaaaat
     3601 tgcaggatct caacattgaa ctggcaagag agctgctccg ggctcatgca cagcttcagc
     3661 gacttaattt ggagttgctt cgcgaactgc ttcgagcttt ggctcaattg caagagctta
     3721 acctcgacct cttgaggttg gcttccgaat tgactgaccc agacgaggcg aggaaagcga
     3781 tcgccgttac aggctaccgg ctctttgaag aaatactgga cagtgagcga ttgagtcggg
     3841 aggccgcagc agcatctgaa aagagcagcc gggaggctga acgccttata cgagaagctg
     3901 cagcggcttc tagcaatact ataagggttt ttctccccaa caagcaacgc accgtggtca
     3961 acgtgaggaa cgggatgtca ctgcacgatt gcctgatgaa ggcgctgaag gttcgaggtt
     4021 tgcaacccga gtgttgcgcg gtgttcaggc ttcttcacga gcataagggg aaaaaagcac
     4081 gattggattg gaacactgat gcagccagtc tcatcggcga ggaattgcag gtggattttt
     4141 tgtgagaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc
     4201 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4261 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4321 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4381 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4441 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4501 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4561 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4621 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4681 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4741 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4801 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4861 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4921 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4981 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5041 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5101 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5161 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     5221 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     5281 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     5341 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5401 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     5461 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     5521 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     5581 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     5641 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5701 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5761 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     5821 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     5881 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5941 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6001 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6061 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6121 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     6181 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6241 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6301 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6361 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6421 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6481 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6541 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6601 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6661 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6721 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6781 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6841 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6901 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6961 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7021 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7081 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7141 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7201 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7261 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7321 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7381 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7441 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7501 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7561 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7621 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7681 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7741 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7801 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7861 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7921 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7981 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8041 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8101 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8161 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8221 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8281 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8341 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8401 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8461 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8521 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8581 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
