LOCUS       sequence-428989-        8616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET-based reporter for Ras activity, untargeted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Zhang JZ, Nguyen WH, Greenwood N, Rose JC, Ong SE, Maly DJ, Baker D
  TITLE     Computationally designed sensors detect endogenous Ras activity and 
            signaling effectors at subcellular resolution.
  JOURNAL   Nat Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w.
  PUBMED    38273065
REFERENCE   2  (bases 1 to 8616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w."
COMMENT     Sequence Label: pcDNA3 Ras-LOCKR-S
FEATURES             Location/Qualifiers
     source          1..8616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      895..904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             913..930
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             937..966
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             976..1005
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1015..1044
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1951..2007
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2008..2031
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2041..2064
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2074..2097
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4207..4224)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4213..4437
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4483..4911
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4570..4589)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4780..4801
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4920..4940)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4925..5254
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5105..5240
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5167..5186
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5321..6115
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5375..5394)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5985..6004
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6289..6410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6326..6345)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6380..6399
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6459..6475)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6459..6475)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6472..6494)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6483..6499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6507..6513,6514..6531,6532..6537))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6552..6573
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6690..6707)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6861..7449)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6941..6960)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7620..8411,8412..8480))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8243..8262
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8481..8585)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggccacc
      901 atggggtcta gccatcacca tcatcaccat tctagcgagc agaaactcat ctctgaagag
      961 gatctcggtg gtagtgaaca aaagttgata tccgaggagg atttgggtgg gagcgagcag
     1021 aaacttatca gtgaagaaga tttggggggc tccatggatc ctgacgaggc aaggaaagcc
     1081 atagcgaggg ttaaaaggga aagcaaacga atagtagaag acgcggaaag gcttagtcga
     1141 gaagcagcgg cggcaagtga gaaatcctcc cgagaggcgg agaggctctc ccgggaggcc
     1201 gctgctgcgt ccgagaaaat ttcccgggag ggaggaacag gggggtcagg agggactgga
     1261 ggctcaatgg tatctaaagg agaagagctg tttactgggg tcgtgcctat cctggtagag
     1321 ctggacggtg acgttaatgg acataagttt agcgtaagtg gggaaggcga aggcgatgct
     1381 acttacggga agttgacttt gaaatttatc tgtaccactg gaaagctgcc ggtaccctgg
     1441 cctacgttgg ttaccacgtt gacctggggt gtccaatgct tctcaagata cccagatcac
     1501 atgaagcaac acgacttttt caaatctgcg atgcccgaag gatatgttca agagagaacg
     1561 atatttttta aagatgatgg taactataaa actagagccg aggtcaagtt cgagggggac
     1621 actctggtta accggatcga acttaaaggc attgacttta aggaggatgg gaacatcctg
     1681 ggccataaat tggagtacaa ctatatatca cataacgtct atatcacggc ggacaagcaa
     1741 aagaatggga taaaggctaa ctttaaaata cggcataaca ttgaggatgg aagcgtgcag
     1801 cttgcggacc attatcaaca aaatacgcct ataggagacg gacccgtgct gctgcctgat
     1861 aatcactatc tttccactca aagtgcgctc tctaaagacc cgaatgagaa acgagaccat
     1921 atggttctgt tggagttcgt aacggcagcg gcaaccaatt tcagtctcct gaagcaggca
     1981 ggtgatgtag aagagaatcc cggaccggat tataaagacg atgatgacaa gggaggtagt
     2041 gattacaagg atgatgacga taaaggaggt tccgattaca aagatgatga cgacaagggc
     2101 gggtctatga agggcgaaga acttttcaca ggcgtggtgc cgatattggt ggaactcgat
     2161 ggagacgtaa atgggcataa attctcagtt tccggggaag gggaaggtga cgccacttat
     2221 gggaagctta ctctcaaatt gctgtgcact acggggaagc ttccagtgcc ttggcctaca
     2281 cttgttacca cactggggta cggagtgcag tgtttcgccc gctaccccga ccacatgaaa
     2341 cagcatgatt tctttaaatc tgcgatgcca gaaggttatg tgcaagagag aactattttt
     2401 ttcaaggatg acggcaatta caaaactagg gcggaggtta agttcgaggg tgacacactc
     2461 gtgaatagga ttgagttgaa gggcatagac tttaaagaag atgggaatat cttgggtcac
     2521 aagcttgagt ataattataa ctcccataat gtctacatca cagcagataa acagaaaaat
     2581 ggaattaaag caaacttcaa gatacgacac aacatcgagg acggtggagt tcagttggcg
     2641 gaccactacc aacaaaatac acctatcggt gacgggccag tccttctgcc cgacaaccac
     2701 tacctctcct atcaaagcgc gcttttcaag gaccctaatg agaagagaga ccatatggtt
     2761 cttcttgaat ttctgacagc agcgggaggt accggcggtt ccgggggtac gggaggcagt
     2821 tcaaaggaag cagcgaagaa actccaggac ctgaatatag agcttgcgcg gaaactcctc
     2881 gaagcgagca cgaagcttca aagacttaac ataaggctgg cagaagctct cttggaagcc
     2941 atcgctagat tgcaggagct gaatctggag ctcgtatacc tggctgttga gctgacagac
     3001 cctaaacgga tacgggatga gatcaaggaa gtcaaagata agagcaagga aattattcgc
     3061 cgcgctgaaa aagaaataga tgatgctgcc aaggagtcca aaaaaattct tgaggaagca
     3121 cgaaaagcta ttcgagacgc cgccgaggag agtaggaaaa ttctcgagga aggtagcggg
     3181 agcgggtcag atgccctgga tgagttgcaa aagttgaatc ttgaacttgc gaagcttctc
     3241 cttaaagcaa ttgctgaaac tcaagacttg aatcttagag ctgcaaaggc ctttctcgaa
     3301 gcggccgcaa aattgcagga acttaatatt agggctgtgg aacttctcgt aaagctcacc
     3361 gacccggcaa ccatacgccg ggctctggag catgctaaga gacggtcaaa ggagataatc
     3421 gacgaagcgg aaagggctat tcgggcagct aagcgcgaga gcgagcgaat tattgaagaa
     3481 gcgcggcgac tcatagagaa ggccaaggag gagagtgaaa ggataattcg agaaggttcc
     3541 ggcagtggtg atcccgatat caaaaaattg caggatctca acattgaact ggcaagagag
     3601 ctgctccggg ctcatgcaca gcttcagcga cttaatttgg agttgcttcg cgaactgctt
     3661 cgagctttgg ctcaattgca agagcttaac ctcgacctct tgaggttggc ttccgaattg
     3721 actgacccag acgaggcgag gaaagcgatc gccgttacag gctaccggct ctttgaagaa
     3781 atactggaca gtgagcgatt gagtcgggag gccgcagcag catctgaaaa gagcagccgg
     3841 gaggctgaac gccttatacg agaagctgca gcggcttcta gcaatactat aagggttttt
     3901 ctccccaaca agcaacgcac cgtggtcaac gtgaggaacg ggatgtcact gcacgattgc
     3961 ctgatgaagg cgctgaaggt tcgaggtttg caacccgagt gttgcgcggt gttcaggctt
     4021 cttcacgagc ataaggggaa aaaagcacga ttggattgga acactgatgc agccagtctc
     4081 atcggcgagg aattgcaggt ggattttttg tgagaattct gcagatatcc atcacagaat
     4141 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct
     4201 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4261 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     4321 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     4381 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     4441 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     4501 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4561 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4621 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4681 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4741 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4801 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4861 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     4921 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4981 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     5041 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     5101 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5161 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     5221 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     5281 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     5341 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     5401 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     5461 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     5521 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     5581 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5641 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5701 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5761 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5821 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     5881 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5941 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     6001 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     6061 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     6121 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     6181 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6241 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     6301 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6361 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     6421 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6481 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6541 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6601 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6661 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6721 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6781 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6841 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6901 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6961 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7021 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7081 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7141 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7201 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7261 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7321 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7381 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7441 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7501 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7561 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7621 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7681 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7741 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7801 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7861 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7921 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7981 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8041 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8101 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8161 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8221 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8281 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8341 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8401 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8461 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8521 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8581 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
