LOCUS       sequence-428996-        6410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses green #2.4 pre-SynapShot and Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Son S, Nagahama K, Lee J, Jung K, Kwak C, Kim J, Noh YW, Kim E, Lee 
            S, Kwon HB, Heo WD
  TITLE     Real-time visualization of structural dynamics of synapses in live 
            cells in vivo.
  JOURNAL   Nat Methods. 2024 Jan 8. doi: 10.1038/s41592-023-02122-4.
  PUBMED    38191933
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2024 Jan 8. doi: 10.1038/s41592-023-02122-4."
COMMENT     Sequence Label: #2.4 pre-G-SynapShot-Myc
FEATURES             Location/Qualifiers
     source          1..6410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   32..161
                     /label=AAV2 ITR
     promoter        474..751
                     /label=chicken beta-actin promoter
     primer_bind     1030..1052
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1114..1133
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(1392..1410)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1660..1679
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1854..1877
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             2864..2893
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    2907..3495
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2960..2980)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3378..3389)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3538..3659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3549..3568)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3603..3622
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4000..4455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4087..4106)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4297..4318
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4481..4585
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4586..4654,4655..5446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4804..4823)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5617..6205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6106..6125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tccccgcgcg ttggccgatt cattaatgca gctgcgcgct cgctcgctca ctgaggccgc
       61 ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
      121 gcgcagagag ggagtggcca actccatcac taggggttcc ttgtagttaa tgattaaccc
      181 gccatgctac ttatctacgt agccatgctc taggaagatc gtaccattga cgtcaataat
      241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      421 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
      481 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
      541 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggcgcgc
      601 gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc
      661 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
      721 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
      781 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
      841 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
      901 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
      961 gtgcgggggg agcggctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     1021 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     1081 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     1141 atcattttgg caaagaattg gatccgccac catgccacct tctactagtc ttttgttgct
     1201 tgctgcactt ctccccttcg ctcttcccgc ttcagattgg aagacaggag aagtcaccat
     1261 ggtgagcaag agcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcttggaggg
     1321 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cttacgaggg
     1381 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     1441 cctgtccccc ctgatcatgt acggctccaa gatgtacgtg aagcaccccg ccgacgtccc
     1501 cgattacatg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgcacttcga
     1561 ggacggcggt ctggtgaccg cgacacagga ctcctccctg caggacggca cgctgatcta
     1621 caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     1681 cttgggctgg gattatgcca ccgagcgcct gtaccccgaa gaaggcgtgc tgaagggcga
     1741 gctcctgggg cgcctgaagc tgaaggacgg cggcctcaac ctggtggagt ccaagaccat
     1801 ctacatggcc aagaagcccg tgcaactgcc cggctactac ttcgtggaca ccaagctgga
     1861 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     1921 ccaccacctg gggcatggta caggtagcac aggcagcggt agctccggta ccctgtacaa
     1981 gggcggtggc ggatctggcg gcggtggtag cgtcgacttt cagaaagcta gctctattgt
     2041 ttataagaag gagggggaac aggtggagtt cagtttccca ttggccttca cagtcgagaa
     2101 gctcacagga tcaggagagt tgtggtggca ggccgaaaga gcctcctcca gcaagagttg
     2161 gatcactttt gatctgaaaa acaaagaagt gagcgtgaag agagtcacac aggaccctaa
     2221 gctgcagatg ggcaagaagt tgcctttgca cttgacactc ccccaagccc tgccccagta
     2281 tgctggctcc gggaacctca ccctcgcctt ggaggctaag accggaaagt tgcatcagga
     2341 ggtcaacctc gtggtgatga gggcaacaca gctgcagaag aacctgacct gcgaagtgtg
     2401 gggtcccacc tcacctaaac tgatgctgtc tctgaagctg gagaataagg aggctaaagt
     2461 cagtaaaaga gagaaggctg tgtgggtcct taacccggag gcagggatgt ggcagtgtct
     2521 tcttagcgac tctggacagg tgctgctgga gagcaacatc aaggtgcttc ctacttggtc
     2581 aacaccagta cagccgatgg ccctgatcgt actcggggga gtcgccggcc tgcttctgtt
     2641 tatcggcctg ggcatcttct tctgcgtacg ctacaagtat cgcaacaggg atgagggatc
     2701 ttaccacgtt gacgaatcta ggaactatat cagtaacagc gctcagagta atggcgccgt
     2761 ggttaaagag aaacagccct ccagcgccaa atcagcaaac aaaaacaaga aaaacaagga
     2821 taaagagtac tacgtaagat ctggaccggt cgccaccgtc gacgaacaaa aactcatctc
     2881 agaagaggat ctgtaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     2941 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3001 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3061 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     3121 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     3181 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     3241 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     3301 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     3361 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3421 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     3481 gggccgcctc cccgcatcga taccgtcgac ccgggcggcc gcttcgagca gacatgataa
     3541 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     3601 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     3661 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     3721 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct agagcatggc
     3781 tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag
     3841 ttggccactc cctctctgcg cgctcgctcg ctcacggcgg ccgcccgggc tttgcccggg
     3901 cggcctcagt gagcgagcga gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc
     3961 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     4021 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4081 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4141 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4201 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4261 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4321 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4381 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4441 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4501 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4561 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4621 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4681 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4741 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4801 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4861 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4921 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4981 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5041 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5101 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5161 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5221 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5281 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5341 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5401 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5461 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5521 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5581 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5641 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5701 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5761 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5821 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5881 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5941 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6001 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6061 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6121 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6181 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6241 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6301 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6361 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
//
