LOCUS       sequence-429005-        4801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iFAST containing plasmid for endogenous fusion constructs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Devos J, Van Dijck P, Van Genechten W
  TITLE     A multi-colour fluorogenic tag and its application in Candida 
            albicans.
  JOURNAL   Microbiology (Reading). 2024 Mar;170(3). doi: 10.1099/mic.0.001451.
  PUBMED    38535895
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology (Reading)."; date: "2024-03"; volume: "170(3). doi"; 
            pages: " 10.1099/mic.0.001451"
COMMENT     Sequence Label: pFA6 - iFAST
FEATURES             Location/Qualifiers
     source          1..4801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2025..2044)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2026..2044)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2131..2148)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2302..2890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2382..2401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3061..3852,3853..3921))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3684..3703
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3922..4026)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4094..4112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4150..4172)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4272..4291
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4372..4390
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4372..4389
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ccattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat
       61 cagttattac ccggggtcaa aactagagaa taataaagaa aacgatcttt tcaaaaagaa
      121 aaaacctttt agttttcctt tgttgttgtt gtgggtgtgt gctatttata ttatatagtt
      181 tactcataat accataaaat attcggtttg attaggttat tttaataagc taatttgttt
      241 ctaatcgtgt aatttatgct gtgtatatta agtagtgtgt gcactgccca aaaatgtttg
      301 ttgtttatag tcggttaaag agaaaaaaga aaaaaagatc catacacaca cgttaattag
      361 ttgttcaacg taatacactc atattttgtt cttatttgct ttcggtcgct gttctcacca
      421 agatttattg ccaacgaaac aatttttttt tatatatttt cagatttttc tttttttcct
      481 ttcctttcct tttctaattt tcactcctgg ttttctttct ttcttagaaa cattatctcg
      541 atattaatat taaaaaaata taatcattca aaatggacgg tggtatgttt tagtttagct
      601 tcaattctaa ttgattgatt aatcagttga ttggtttcaa tatgacaaat gggtagggtg
      661 ggaaaacttc attttcaatt cagatcaaac ttttttgttg tcgacataat atttctcgtt
      721 tgggatgtta ctgtcacatt aataatacac acacatcagc ttataatttt gaaagtaatt
      781 tatcagatat gttgtgacga tcagtggaaa tggctaactt caatgtatct gttcttcccc
      841 tttttcaaag ttcacgtttt ttgattgatt gattgatctg tcggcagtgg tttcaaaacc
      901 attcggtgag taatcctatc aatcaatgtt acgacaaaag gctcaatatt caaaattgca
      961 atgttttatg ttttcctacg tgtacttgtg caaggcaatt gattcaacat tgcttttggt
     1021 gtttgacgag tttctagttt ggacttgtgt tgttatctgg gctatacaga tttcccggct
     1081 cactatgaat tttttttttc gacgctcagt gcacacaact ataaacaaca caaacacaaa
     1141 cacagcaaga aaaaaaaaaa acgaacattg aattgaaacc aagccaactg aaaaattcct
     1201 tatttaaatg actgtcatac taacccattt ttatagaaga agttgctgct ttagttatcg
     1261 ataacggttc tcatatgaaa atttcggtga tccctgagca ggtggcggaa acattggatg
     1321 ctgagaacca tttcattgtt cgtgaagtgt tcgatgtgca cctatccgac caaggctttg
     1381 aactatctac cagaagtgtg agcccctacc ggaaggatta catctcggat gatgactctg
     1441 atgaagactc tgcttgctat ggcgcattca tcgaccaaga gcttgtcggg aagattgaac
     1501 tcaactcaac atggaacgat ctagcctcta tcgaacacat tgttgtgtcg cacacgcacc
     1561 gaggcaaagg agtcgcgcac agtctcatcg aatttgcgaa aaagtgggca ctaagcagac
     1621 agctccttgg catacgatta gagacacaaa cgaacaatgt acctgcctgc aatttgtacg
     1681 caaaatgtgg ctttactctc ggcggcattg acctcttcac gtataaaact agacctcaag
     1741 tctcgaacga aacagcgatg tactggtact ggttctcggg agcacaggat gacgcctaac
     1801 atatgtgaag tgtgaagggg gagattttca ctttattaga tttgtatata tgtataataa
     1861 ataaataaat aagttaaata aataattaga taagggtggt aattattact atttacaatc
     1921 aaaggtggtc ctgcactcga attcatcgat gatatcagag agctcgaatt catcgatgat
     1981 atcagatcca ctagtggcct atgcggccgc ggatctgccg gtctccctat agtgagtcgt
     2041 attaatttcg ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg
     2101 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2161 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2221 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2281 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2341 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2401 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2461 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2521 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2581 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2641 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2701 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2761 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2821 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2881 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2941 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3001 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3061 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3121 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3181 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3241 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3301 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3361 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3421 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3481 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3541 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3601 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3661 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3721 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3781 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3841 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3901 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3961 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4021 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     4081 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     4141 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     4201 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     4261 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgg acatattgtc
     4321 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
     4381 acactataga acgcggccgc cagctgaagc ttcgtacgct gcaatggaac atgttgcctt
     4441 tggtagcgaa gatatcgaga atactctagc gaaaatggat gatggccaat tagatggatt
     4501 agcctttggt gctatacagc ttgatggtga tggcaatatt ttacagtata atgcagctga
     4561 aggagacata acagggagag atcccaaaca agtgattggc aagaacttct tcaaagacgt
     4621 agcaccaggt acagattctc ctgaatttta cgggaaattc aaggaaggag tagcatcagg
     4681 taacttgaat accatgttcg agtggatgat tccgacgagt agggggccaa ccaaggttaa
     4741 aattcacatg aagaaggctt tgagtggtga ctcctattgg gtgtttgtca aaagggttta
     4801 a
//
