LOCUS       sequence-429006-        9849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains human codon optimized sequence of EcSTH which 
            can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio
            in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Yang R, Yang C, Ma L, Zhao Y, Guo Z, Niu J, Chu Q, Ma Y, Li B
  TITLE     Identification of purine biosynthesis as an NADH-sensing pathway to 
            mediate energy stress.
  JOURNAL   Nat Commun. 2022 Nov 17;13(1):7031. doi: 10.1038/s41467-022-34850-0.
  PUBMED    36396642
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-11-17"; volume: "13(1):7031. doi"; pages: " 
            10.1038/s41467-022-34850-0"
COMMENT     Sequence Label: EcSTH
FEATURES             Location/Qualifiers
     source          1..9849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2206..2555
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    2214..2232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2286..2304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2322..2340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2358..2376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2394..2412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2430..2448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2494..2518
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2565..2630
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      2715..2724
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4073..4646
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4096..4648
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4240..4257)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4467..4486
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        4682..5181
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4717..4740)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5114..5133
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5202..5801
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5202..5221)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5698..5718
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5818..6406
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5871..5891)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6289..6300)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6613..7246
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7374..7390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7374..7390)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7387..7409)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7398..7414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7422..7428,7429..7446,7447..7452))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7467..7488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7605..7622)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7776..8364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7856..7875)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8535..9326,9327..9395))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9158..9177
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9396..9500)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9548..9682
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9585..9604)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9639..9658
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag
     2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
     2521 ctggagcaat tccacaacac ttttgtctta tacttggatc catggactac aaagaccatg
     2581 acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag ggaggaggag
     2641 gatcaggagg aggaatgccc cactcctacg actacgacgc catcgtgatc ggctccggcc
     2701 ccggcggcga gggcgccgcc atgggcctgg tgaagcaggg cgcccgcgtg gccgtgatcg
     2761 agcgctacca gaacgtgggc ggcggctgca cccactgggg caccatcccc tccaaggccc
     2821 tgcgccacgc cgtgtcccgc atcatcgagt tcaaccagaa ccccctgtac tccgaccact
     2881 cccgcctgct gcgctcctcc ttcgccgaca tcctgaacca cgccgacaac gtgatcaacc
     2941 agcagacccg catgcgccag ggcttctacg agcgcaacca ctgcgagatc ctgcagggca
     3001 acgcccgctt cgtggacgag cacaccctgg ccctggactg ccccgacggc tccgtggaga
     3061 ccctgaccgc cgagaagttc gtgatcgcct gcggctcccg cccctaccac cccaccgacg
     3121 tggacttcac ccacccccgc atctacgact ccgactccat cctgtccatg caccacgagc
     3181 cccgccacgt gctgatctac ggcgccggcg tgatcggctg cgagtacgcc tccatcttcc
     3241 gcggcatgga cgtgaaggtg gacctgatca acacccgcga ccgcctgctg gccttcctgg
     3301 accaggagat gtccgactcc ctgtcctacc acttctggaa ctccggcgtg gtgatccgcc
     3361 acaacgagga gtacgagaag atcgagggct gcgacgacgg cgtgatcatg cacctgaagt
     3421 ccggcaagaa gctgaaggcc gactgcctgc tgtacgccaa cggccgcacc ggcaacaccg
     3481 actccctggc cctgcagaac atcggcctgg agaccgactc ccgcggccag ctgaaggtga
     3541 actccatgta ccagaccgcc cagccccacg tgtacgccgt gggcgacgtg atcggctacc
     3601 cctccctggc ctccgccgcc tacgaccagg gccgcatcgc cgcccaggcc ctggtgaagg
     3661 gcgaggccac cgcccacctg atcgaggaca tccccaccgg catctacacc atccccgaga
     3721 tctcctccgt gggcaagacc gagcagcagc tgaccgccat gaaggtgccc tacgaggtgg
     3781 gccgcgccca gttcaagcac ctggcccgcg cccagatcgt gggcatgaac gtgggcaccc
     3841 tgaagatcct gttccaccgc gagaccaagg agatcctggg catccactgc ttcggcgagc
     3901 gcgccgccga gatcatccac atcggccagg ccatcatgga gcagaagggc ggcggcaaca
     3961 ccatcgagta cttcgtgaac accaccttca actaccccac catggccgag gcctaccgcg
     4021 tggccgccct gaacggcctg aaccgcctgt tctaggcggc cgccccggcc ggcccctctc
     4081 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     4141 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     4201 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     4261 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     4321 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     4381 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     4441 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     4501 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct
     4561 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     4621 ttttcctttg aaaaacacga tgataatatg gcccccggga cgcgtcatat ggaattctac
     4681 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     4741 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     4801 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     4861 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     4921 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     4981 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     5041 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     5101 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     5161 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
     5221 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     5281 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     5341 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5401 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5461 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5521 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     5581 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     5641 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     5701 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     5761 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgggcgcgt ctggaacaat
     5821 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5881 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5941 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6001 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6061 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6121 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6181 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     6241 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6301 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6361 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
     6421 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     6481 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     6541 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6601 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     6661 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     6721 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     6781 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     6841 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     6901 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     6961 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     7021 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     7081 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7141 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7201 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7261 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     7321 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7381 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7441 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7501 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7561 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7621 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7681 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7741 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7801 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7861 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7921 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7981 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8041 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8101 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8161 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8221 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8281 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8341 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8401 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8461 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8521 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8581 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8641 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8701 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8761 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8821 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8881 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8941 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9001 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9061 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9121 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9181 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9241 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9301 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9361 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9421 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9481 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     9541 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9601 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9661 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     9721 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     9781 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     9841 cttagtagt
//
