LOCUS       sequence-429008-        2991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes GFP under the control of synthetic promoter responsive to 
            JUB1-derived artificial transcription factor in bacterial cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Naseri G, Raasch H, Charpentier E, Erhardt M
  TITLE     A versatile regulatory toolkit of arabinose-inducible artificial 
            transcription factors for Enterobacteriaceae.
  JOURNAL   Commun Biol. 2023 Oct 3;6(1):1005. doi: 10.1038/s42003-023-05363-3.
  PUBMED    37789111
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-10-3"; volume: "6(1):1005. doi"; pages: " 
            10.1038/s42003-023-05363-3"
COMMENT     Sequence Label: pJUB2x
FEATURES             Location/Qualifiers
     source          1..2991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        186..220
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
     RBS             230..241
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      987..1073
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1110..1127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1323..1342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1826..1920)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1950..2741,2742..2810))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2573..2592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2811..2915)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(2983..2991,1..10)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atcacgaggc cctttcgtct tcacctcgag gatcccgggt acctgcagct agcgtcgact
       61 gtggtctcta caggaattct ttcttctgtc gccgtcttgt caacggcacc agttatatat
      121 ttcttctgtc gccgtcttgt caacggcacc agttatatat tgggttccac cggatacctc
      181 cggacttgac agctagctca gtcctaggga ttgtgctagc gaattcatta aagaggagaa
      241 aggtaccatg tctaaaggtg aagaactgtt caccggtgtt gttccgatcc tggttgaact
      301 ggatggtgat gttaacggcc acaaattctc tgttcgtggt gaaggtgaag gtgatgcaac
      361 caacggtaaa ctgaccctga aattcatctg cactaccggt aaactgccgg ttccatggcc
      421 gactctggtg actaccctga cctatggtgt tcagtgtttt tctcgttacc cggatcacat
      481 gaagcagcat gatttcttca aatctgcaat gccggaaggt tatgtacagg agcgcaccat
      541 ttctttcaaa gacgatggca cctacaaaac ccgtgcagag gttaaatttg aaggtgatac
      601 tctggtgaac cgtattgaac tgaaaggcat tgatttcaaa gaggacggca acatcctggg
      661 ccacaaactg gaatataact tcaactccca taacgtttac atcaccgcag acaaacagaa
      721 gaacggtatc aaagctaact tcaaaattcg ccataacgtt gaagacggta gcgtacagct
      781 ggcggaccac taccagcaga acactccgat cggtgatggt ccggttctgc tgccggataa
      841 ccactacctg tccacccagt ctgttctgtc caaagacccg aacgaaaagc gcgaccacat
      901 ggtgctgctg gagttcgtta ctgcagcagg tatcacgcac ggcatggatg agctctacaa
      961 atgataagcg gccgctctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1021 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
     1081 cctagaccta ggcggagata ttccgcttcc tcgctcactg actcgctccg ctcggtcgtt
     1141 cggctgcggc gagcggtacc ggcttactgg cggggcggaa atttcctgga agatgccagg
     1201 aagaaactta acagggaagc gataaggcca cggcagagcc gtttttccat aggctccgcc
     1261 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     1321 ttaaagatac caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt
     1381 tcggtttacc ggtgtcactc cgctgttatg gccgcgttta tctcattcca cgcctgacac
     1441 tcggttccgg gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     1501 ccgactgctg cgcctgttcc ggtaactatc gacttgagtc caagccggaa agacacgaca
     1561 aagcgccact ggcagcagcc actggtaaga gggtgtacaa gagattactg atgcagagtt
     1621 cttgaagtca gagccaaact acggctaaac tggaaggacg aatttggtgg cagcggtctt
     1681 gtacaccctg ttaccacggt tcagaagttc cccaacttac tgaaccttcg aaaaaccacc
     1741 tccccaggtg gttttttcgt tttcagagca agagattacg acgcaacgga aaggatctca
     1801 agaggttcat cttactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     1861 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     1921 caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1981 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2041 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2101 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2161 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2221 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2281 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2341 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2401 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2461 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2521 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2581 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2641 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2701 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2761 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2821 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2881 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2941 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg t
//
