LOCUS       sequence-429009-       12916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC 
            cassette-35S-hptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Zhou G, Niu R, Zhou Y, Luo M, Peng Y, Wang H, Wang Z, Xu G
  TITLE     Proximity editing to identify RNAs in phase-separated RNA binding 
            protein condensates.
  JOURNAL   Cell Discov. 2021 Aug 24;7(1):72. doi: 10.1038/s41421-021-00288-9.
  PUBMED    34426573
REFERENCE   2  (bases 1 to 12916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-08-24"; volume: "7(1):72. doi"; pages: " 
            10.1038/s41421-021-00288-9"
COMMENT     Sequence Label: pZG162
FEATURES             Location/Qualifiers
     source          1..12916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        216..262
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             291..542
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             558..791
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             801..1736
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             849..1742
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        2300..2479
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2332..2515
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      3630..3882
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4203..4249
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(6117..6134)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6117..6133)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6126..6148)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6355..6379
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6900..7691
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7937..8525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8426..8445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8679..8696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8711..8851
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8777..8796)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8937..8959
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9195..9389
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9455..10528)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10957..11586)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
      121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
      181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      241 agttcatttc atttggagag gacacgctgg gatccccaat tccgggcgga atgaaagcgt
      301 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
      361 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
      421 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
      481 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
      541 ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
      601 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
      661 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
      721 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
      781 agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg ccaagcccgc
      841 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
      901 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
      961 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
     1021 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
     1081 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
     1141 tggagcaccc agggaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
     1201 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
     1261 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
     1321 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
     1381 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
     1441 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
     1501 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
     1561 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
     1621 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
     1681 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
     1741 gagagctccc tggcggaatt cccagagatg ttagctgaaa tcatcactaa tcagatacca
     1801 aaatattcaa atggaaatat caaaaagctt ctgtttcatc aaaaatgact cgacctaact
     1861 gagtaagcta gcttgttcga gtattatggc attgggaaaa ctgtttttct tgtaccattt
     1921 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
     1981 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
     2041 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
     2101 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
     2161 agtaaaacac tagatcccca aacaagcttg aaactgaagg cgggaaacga caatctgatc
     2221 atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt
     2281 tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg
     2341 gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta
     2401 aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat
     2461 tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat ctgcaccgga
     2521 tccgctagag gatctcgacc tgcaagatcc cggggggcaa tgagatatga aaaagcctga
     2581 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     2641 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     2701 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     2761 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     2821 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     2881 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     2941 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     3001 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     3061 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     3121 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     3181 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     3241 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     3301 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     3361 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     3421 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     3481 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     3541 cgccgatagt ggaaaccgac gccccagcac tcgtccggga tcttggaggt gatgtaacat
     3601 gatcacaagc tgatcccccg aatttccccg atcgttcaaa catttggcaa taaagtttct
     3661 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     3721 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     3781 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     3841 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg atcccccctc
     3901 gacagcttgc atgccgcttg ggctgcaggt cgaggctaaa aaactaatcg cattatcatc
     3961 ccctcgacgt actgtacata taaccactgg ttttatatac agcagtactg tacatataac
     4021 cactggtttt atatacagca gtcgacgtac tgtacatata accactggtt ttatatacag
     4081 cagtactgta catataacca ctggttttat atacagcagt cgaggtaaga ttagatatgg
     4141 atatgtatat ggatatgtat atggtggtaa tgccatgtaa tatgctcgac tctaggatct
     4201 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaagc
     4261 tagtcgactc tagcctcgag gagctcacca tgtactctcc tatggtcgta ccgcaaaaaa
     4321 atgtgcctct ccctatagac gataaatcta gctccatgga gcttccgcag atccacgcag
     4381 acactatcgg tcgattggtg ctggaaaagt tcatggaggt tattaaaggt caggaggctt
     4441 atagccgtcg aaaagttttg gctggaatcg taatgaccga aaacatgaac ttctgtgagg
     4501 caaaagtcat ttcagtgtcc acgggcacca agtgtgtgtc cggcgagcat atgtccgtaa
     4561 acggtgcggt actcaatgac agccacgccg agatagtgtc acgtcgttgt ctcctcaagt
     4621 atctctatgc ccaactggat cttcaatgca accaggcgac cgcttatcag agcatatttg
     4681 tgcgtaatac tgacggtcag tatccctata aactgaagtc tggggtccat tttcatctct
     4741 atataaacac ggcgccctgc ggcgatgccc gtattttttc acctcacgag aatgacacgg
     4801 gagtagataa acatcccaac cgaaaagcaa gagggcagct ccgaaccaaa atcgaatccg
     4861 gccagggcac aatcccagtg aaatctagcg acggcattca aacttgggac ggcgtactac
     4921 aaggccagcg tttgcttacg atgtcctgct ccgataaaat tgctaggtgg aacatcgtcg
     4981 gtatccaggg ctccttgctc tcttcaatta tagagcccgt ctatctgcat tcaattgtct
     5041 tagggtcact cttgcatcct gaacatatgt accgagccgt ttgtgggaga atagaaaaga
     5101 gtatccaggg tctaccccca ccttaccatt tgaacaaacc cagactggca ttggtcacat
     5161 ccgcagaacc acgtaaccaa gccaaggccc cgaattttgg aataaactgg actataggtg
     5221 acacggaact agaggttgtg aactctctca caggcagaac cataggaggg caagtcagcc
     5281 gtataacgaa gcaggctttt ttcgtaaagt atggtttctt gatggctaat ttgcccggaa
     5341 tcctcgtccg aaaggtaacg accgattacg gtcagacaaa agcgaatgta aaggattacc
     5401 agatcgcgaa gttggaattg ttctcagcct tcaagaggga ggatcttgga tcatggctaa
     5461 agaaaccaat agagcaggac gagtttggat tggctgaatc tagaggaggt ggctcatctg
     5521 gcggaggtca gatctcgtac gcgtcccggg gcggtacctt cgacgacaag accgggcccg
     5581 gctcttctcc tcactcgacc tgcactagtc gatccaggcc tcccagcttt cgtccgtatc
     5641 atcggtttcg acaacgttcg tcaagttcaa tgcatcagtt tcattgccca cacaccagaa
     5701 tcctactaag tttgagtatt atggcattgg aaaagctgtt ttcttctatc atttgttctg
     5761 cttgtaattt actgtgttct ttcagttttt gttttcggac atcaaaatgc aaatggatgg
     5821 ataagagtta ataaatgata tggtcctttt gttcattctc aaattattat tatctgttgt
     5881 ttttacttta atgggttgaa tttaagtaag aaaggaacta acagtgtgat attaaggtgc
     5941 aatgttagac atataaaaca gtctttcacc tctctttggt tatgtcttga attggtttgt
     6001 ttcttcactt atctgtgtaa tcaagtttac tatgagtcta tgatcaagta attatgcaat
     6061 caagttaagt acagtatagg ctttttgtgt cgagggggta ccgagtcgag gaattcactg
     6121 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6181 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6241 tcccaacagt tgcgcagcct gaatggcggg taccgagctc gaattcaatt cggcgttaat
     6301 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     6361 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     6421 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     6481 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     6541 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     6601 gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta
     6661 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     6721 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     6781 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     6841 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     6901 tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     6961 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     7021 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     7081 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     7141 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     7201 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     7261 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     7321 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     7381 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     7441 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     7501 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     7561 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     7621 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     7681 tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     7741 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
     7801 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat
     7861 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     8101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8641 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8701 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     8761 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     8821 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     8881 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8941 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     9001 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     9061 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     9121 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     9181 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     9241 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     9301 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     9361 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     9421 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     9481 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     9541 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     9601 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     9661 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     9721 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     9781 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     9841 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     9901 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     9961 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
    10021 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
    10081 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
    10141 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
    10201 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
    10261 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
    10321 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
    10381 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
    10441 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
    10501 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
    10561 attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
    10621 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
    10681 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
    10741 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
    10801 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
    10861 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
    10921 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
    10981 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
    11041 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
    11101 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
    11161 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
    11221 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
    11281 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
    11341 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
    11401 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
    11461 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
    11521 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
    11581 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
    11641 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
    11701 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
    11761 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
    11821 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
    11881 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
    11941 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
    12001 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
    12061 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
    12121 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
    12181 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
    12241 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
    12301 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
    12361 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
    12421 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
    12481 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
    12541 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
    12601 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
    12661 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
    12721 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttaaagta
    12781 ctttaaagta ctttaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
    12841 caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa
    12901 cgctcttttc tcttag
//
