LOCUS       sequence-42901-e        1219 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CmCitrine
FEATURES             Location/Qualifiers
     source          1..1219
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        173..191
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     173..190
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(277..279,280..282,283..993)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(322..343)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(583..602)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     930..951
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1047..1181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1071..1090)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1125..1144
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cccgcgatgg ctgccaagta catcccccat tgacgtcaat ggggaggggc aatgacgcaa
       61 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
      121 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
      181 gacactatag aatacaagct acttgttctt tttgcaggat ccactagtgg cgcgccatta
      241 attaaaggcc ggccagcgat cgccggacat cgattgatgg tgagcaaggg cgaggagctg
      301 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      361 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      421 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
      481 ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      541 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      601 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      661 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      721 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
      781 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      841 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
      901 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      961 gggatcactc tcggcatgga cgagctgtac aagtaagaat tcaaggcctc tcgagcctct
     1021 agaactatag tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg
     1081 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     1141 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     1201 attttatgtt tcaggttca
//
