LOCUS       sequence-429010-       10912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, 
            followed by IRES-dsRed Express2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Saito Y, Chapple RH, Lin A, Kitano A, Nakada D
  TITLE     AMPK Protects Leukemia-Initiating Cells in Myeloid Leukemias from 
            Metabolic Stress in the Bone Marrow.
  JOURNAL   Cell Stem Cell. 2015 Sep 29. pii: S1934-5909(15)00374-4. doi: 
            10.1016/j.stem.2015.08.019.
  PUBMED    26440282
REFERENCE   2  (bases 1 to 10912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Sep 29. pii: S1934-5909(15)00374-4. doi: 
            10.1016/j.stem.2015.08.019."
COMMENT     Sequence Label: pMIR-FLAG-MLL-AF9
FEATURES             Location/Qualifiers
     source          1..10912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             181..697
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    761..1102
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1169..1585
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1515..1537
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1553..1569
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1616..1639
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6179..6731
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6323..6340)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6550..6569
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6743..7420
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(6923..6943)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7141..7160
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7335..7358
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     LTR             7487..8001
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8163..8179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8187..8193,8194..8211,8212..8217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8232..8253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8370..8387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8541..9129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8621..8640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9300..10091,10092..10160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9923..9942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10161..10265)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10333..10351
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10389..10411)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10511..10530
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10724..10746
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10874..10893
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc aatttgttaa
       61 agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc
      121 tatagagtac gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg
      181 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
      241 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      301 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      361 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      421 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      481 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      541 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      601 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      661 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      721 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      781 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      841 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      901 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      961 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
     1021 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
     1081 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
     1141 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1201 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1261 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1321 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1381 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1441 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1501 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1561 agccctcact ccttctctag gcgccggaat tagatctctc gaggttaatt ccatggacta
     1621 caaggacgac gatgacaagg gcaattccgc gaacatggcg cacagctgtc ggtggcgctt
     1681 ccccgcccga cccgggacca ccgggggcgg cggcggcggg gggcgccggg gcctaggggg
     1741 cgccccgcgg caacgcgtcc cggccctgct gcttcccccc gggcccccgg tcggcggtgg
     1801 cggccccggg gcgcccccct cccccccggc tgtggcggcc gcggcggcgg cggcgggaag
     1861 cagcggggct ggggttccag ggggagcggc cgccgcctca gcagcctcct cgtcgtccgc
     1921 ctcgtcttcg tcttcgtcat cgtcctcagc ctcttcaggg ccggccctgc tccgggtggg
     1981 cccgggcttc gacgcggcgc tgcaggtctc ggccgccatc ggcaccaacc tgcgccggtt
     2041 ccgggccgtg tttggggaga gcggcggggg aggcggcagc ggagaggatg agcaattctt
     2101 aggttttggc tcagatgaag aagtcagagt gcgaagtccc acaaggtctc cttcagttaa
     2161 aactagtcct cgaaaacctc gtgggagacc tagaagtggc tctgaccgaa attcagctat
     2221 cctctcagat ccatctgtgt tttcccctct aaataaatca gagaccaaat ctggagataa
     2281 gatcaagaag aaagattcta aaagtataga aaagaagaga ggaagacctc ccaccttccc
     2341 tggagtaaaa atcaaaataa cacatggaaa ggacatttca gagttaccaa agggaaacaa
     2401 agaagatagc ctgaaaaaaa ttaaaaggac accttctgct acgtttcagc aagccacaaa
     2461 gattaaaaaa ttaagagcag gtaaactctc tcctctcaag tctaagttta agacagggaa
     2521 gcttcaaata ggaaggaagg gggtacaaat tgtacgacgg agaggaaggc ctccatcaac
     2581 agaaaggata aagacccctt cgggtctcct cattaattct gaactggaaa agccccagaa
     2641 agtccggaaa gacaaggaag gaacacctcc acttacaaaa gaagataaga cagttgtcag
     2701 acaaagccct cgaaggatta agccagttag gattattcct tcttcaaaaa ggacagatgc
     2761 aaccattgct aagcaactct tacagagggc aaaaaagggg gctcaaaaga aaattgaaaa
     2821 agaagcagct cagctgcagg gaagaaaggt gaagacacag gtcaaaaata ttcgacagtt
     2881 catcatgcct gttgtcagtg ctatctcctc gcggatcatt aagacccctc ggcggtttat
     2941 agaggatgag gattatgacc ctccaattaa aattgcccga ttagagtcta caccgaatag
     3001 tagattcagt gccccgtcct gtggatcttc tgaaaaatca agtgcagctt ctcagcactc
     3061 ctctcaaatg tcttcagact cctctcgatc tagtagcccc agtgttgata cctccacaga
     3121 ctctcaggct tctgaggaga ttcaggtact tcctgaggag cggagcgata cccctgaagt
     3181 tcatcctcca ctgcccattt cccagtcccc agaaaatgag agtaatgata ggagaagcag
     3241 aaggtattca gtgtcggaga gaagttttgg atctagaacg acgaaaaaat tatcaactct
     3301 acaaagtgcc ccccagcagc agacctcctc gtctccacct ccacctctgc tgactccacc
     3361 gccaccactg cagccagcct ccagtatctc tgaccacaca ccttggctta tgcctccaac
     3421 aatcccctta gcatcaccat ttttgcctgc ttccactgct cctatgcaag ggaagcgaaa
     3481 atctattttg cgagaaccga catttaggtg gacttcttta aagcattcta ggtcagagcc
     3541 acaatacttt tcctcagcaa agtatgccaa agaaggtctt attcgcaaac caatatttga
     3601 taatttccga ccccctccac taactcccga ggacgttggc tttgcatctg gtttttctgc
     3661 atctggtacc gctgcttcag cccgattgtt ttcgccactc cattctggaa caaggtttga
     3721 tatgcacaaa aggagccctc ttctgagagc tccaagattt actccaagtg aggctcactc
     3781 tagaatattt gagtctgtaa ccttgcctag taatcgaact tctgctggaa catcttcttc
     3841 aggagtatcc aatagaaaaa ggaaaagaaa agtgtttagt cctattcgat ctgaaccaag
     3901 atctccttct cactccatga ggacaagaag tggaaggctt agtagttctg agctctcacc
     3961 tctcaccccc ccgtcttctg tctcttcctc gttaagcatt tctgttagtc ctcttgccac
     4021 tagtgcctta aacccaactt ttacttttcc ttctcattcc ctgactcagt ctggggaatc
     4081 tgcagagaaa aatcagagac caaggaagca gactagtgct ccggcagagc cattttcatc
     4141 aagtagtcct actcctctct tcccttggtt taccccaggc tctcagactg aaagagggag
     4201 aaataaagac aaggcccccg aggagctgtc caaagatcga gatgctgaca agagcgtgga
     4261 gaaggacaag agtagagaga gagaccggga gagagaaaag gagaataagc gggagtcaag
     4321 gaaagagaaa aggaaaaagg gatcagaaat tcagagtagt tctgctttgt atcctgtggg
     4381 tagggtttcc aaagagaagg ttgttggtga agatgttgcc acttcatctt ctgccaaaaa
     4441 agcaacaggg cggaagaagt cttcatcaca tgattctggg actgatatta cttctgtgac
     4501 tcttggggat acaacagctg tcaaaaccaa aatacttata aagaaaggga gaggaaatct
     4561 ggaaaaaacc aacttggacc tcggcccaac tgccccatcc ctggagaagg agaaaaccct
     4621 ctgcctttcc actccttcat ctagcactgt taaacattcc acttcctcca taggctccat
     4681 gttggctcag gcagacaagc ttccaatgac tgacaagagg gttgccagcc tcctaaaaaa
     4741 ggccaaagct cagctctgca agattgagaa gagtaagagt cttaaacaaa ccgaccagcc
     4801 caaagcacag ggtcaagaaa gtgactcatc agagacctct gtgcgaggac cccggattaa
     4861 acatgtctgc agaagagcag ctgttgccct tggccgaaaa cgagctgtgt ttcctgatga
     4921 catgcccacc ctgagtgcct taccatggga agaacgagaa aagattttgt cttccatggg
     4981 gaatgatgac aagtcatcaa ttgctggctc agaagatgct gaacctcttg ctccacccat
     5041 caaaccaatt aaacctgtca ctagaaacaa ggcaccccag gaacctccag taaagaaagg
     5101 acgtcgatcg aggcggtgtg ggcagtgtcc cggctgccag gtgcctgagg actgtggtgt
     5161 ttgtactaat tgcttagata agcccaagtt tggtggtcgc aatataaaga agcagtgctg
     5221 caagatgaga aaatgtcaga atctacaatg gatgccttcc aaagcctacc tgcagaagca
     5281 agctaaagct gtgaaaaaga aagagaaaaa gtctaagacc agtgaaaaga aagacagcaa
     5341 agagagcagt gttgtgaaga acgtggtgga ctctagtcag aaacctaccc catcagcaag
     5401 agaggatcct gccccaaaga aaagcagtag tgagcctcct ccacgaaagc ccgtcgagga
     5461 aaagagtgaa gaagggaatg tctcggcccc tgggcctgaa tccaaacagg ccaccactcc
     5521 agcttccagg aagtcaagca agcaggtctc ccagccagca ctggtcatcc cgcctcagcc
     5581 acctactaca ggaccgccaa gaaaagaagt tcccaaaacc actcctagtg agcccaagaa
     5641 aaagcagcct ccaccaccag aatcaggtcc agagcagagc aaacagaaaa aagtggctcc
     5701 ccgcccaagt atccctgtaa aacaaaaacc aaaagaaaag gaaaaaccac ctccggtcaa
     5761 taagcaggag aatgcaggca ctttgaacat cctcagcact ctctccaatg gcaatagttc
     5821 taagcaaaaa attccagcag atgggtcgcg aagtatacac acgtgtcttg aagtgaaaag
     5881 tccaataaag caaagcaaat cagataagca aataaagaat ggtgaatgtg acaaggcata
     5941 cctagatgaa ctggtagagc ttcacagaag gttaatgaca ttgagagaaa gacacattct
     6001 gcagcagatc gtgaacctta tagaagaaac tggacacttt catatcacaa acacaacatt
     6061 tgattttgat ctttgctcgc tggacaaaac cacagtccgt aaactacaga gttacctgga
     6121 aacatctgga acatcctgac tcgaggttaa cgaattccgc cccccccccc cccccctaac
     6181 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     6241 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     6301 agcattccta gggctctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     6361 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     6421 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     6481 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     6541 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     6601 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     6661 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     6721 cgatgataat atggccacaa ccatggatag cactgagaac gtcatcaagc ccttcatgcg
     6781 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     6841 cgagggcaag ccctacgagg gcacccagac cgccaagctg caggtgacca agggcggccc
     6901 cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt
     6961 gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg
     7021 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     7081 gcaggacggc accttcatct accacgtgaa gttcatcggc gtgaacttcc cctccgacgg
     7141 ccccgtaatg cagaagaaga ctctgggctg ggagccctcc accgagcgcc tgtacccccg
     7201 cgacggcgtg ctgaagggcg agatccacaa ggcgctgaag ctgaagggcg gcggccacta
     7261 cctggtggag ttcaagtcaa tctacatggc caagaagccc gtgaagctgc ccggctacta
     7321 ctacgtggac tccaagctgg acatcacctc ccacaacgag gactacaccg tggtggagca
     7381 gtacgagcgc gccgaggccc gccaccacct gttccagtag gtcgacctgc agccaagctt
     7441 atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     7501 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaatacataa
     7561 ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa tatgggccaa
     7621 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
     7681 agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
     7741 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
     7801 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg
     7861 ccagtcctcc gatagactgc gtcgcccggg tacccgtgta tccaataaac cctcttgcag
     7921 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
     7981 ccgtcagcgg gggtctttca tgggtaacag tttcttgaag ttggagaaca acattctgag
     8041 ggtaggagtc gaatattaag taatcctgac tcaattagcc actgttttga atccacatac
     8101 tccaatactc ctgaaatagt tcattatgga cagcgcagaa agagctgggg agaattgtga
     8161 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8221 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8281 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10261 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    10321 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
    10381 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    10441 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
    10501 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    10561 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
    10621 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    10681 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    10741 aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
    10801 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
    10861 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gt
//
