LOCUS       sequence-42902-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NmCitrine
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        219..237
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     219..236
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(284..286,287..289,290..802)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(329..350)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(590..609)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     937..958
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagggtacat tggcagtact cccattgacg tcaatggcgg taaatggccc gcgatggctg
       61 ccaagtacat ccccattgac gtcaatgggg aggggcaatg acgcaaatgg gcgttccatt
      121 gacgtaaatg ggcggtaggc gtgcctaatg ggaggtctat ataagcaatg ctcgtttagg
      181 gaaccgccat tctgcctggg gacgtcggag caagcttgat ttaggtgaca ctatagaata
      241 caagctactt gttctttttg caggatccac tagtcatcga ttgatggtga gcaagggcga
      301 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      361 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      421 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
      481 ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      541 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      601 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      661 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      721 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      781 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      841 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
      901 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      961 cgccgccggg atcactctcg gcatggacga gctt
//
