LOCUS       sequence-429023-        9842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Wiesner SM, Decker SA, Larson JD, Ericson K, Forster C, Gallardo JL,
            Long C, Demorest ZL, Zamora EA, Low WC, SantaCruz K, Largaespada DA,
            Ohlfest JR
  TITLE     De novo induction of genetically engineered brain tumors in mice 
            using plasmid DNA.
  JOURNAL   Cancer Res. 2009 Jan 15. 69(2):431-9.
  PUBMED    19147555
REFERENCE   2  (bases 1 to 9842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2009 Jan 15. 69(2):431-9."
COMMENT     Sequence Label: PT2/C-Luc//PGK-SB13
FEATURES             Location/Qualifiers
     source          1..9842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        129..508
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        510..787
                     /label=chicken beta-actin promoter
     intron          788..1804
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1728..1750
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1812..1831
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1859..3511
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1925..1943)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(3660..3679)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3725..3780
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3779..3798)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4483..4503)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4483..4501)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4522..4538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4522..4538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4535..4557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4546..4562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4570..4576,4577..4594,4595..4600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4615..4636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4753..4770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4924..5512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5004..5023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5683..6474,6475..6543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6306..6325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6544..6648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6716..6734
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     6894..6913
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7122..7138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7174..7673
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     7606..7625
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     intron          7741..7837
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             7904..8926
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKKIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTAAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGRSARKKPLLQNRHKKARLRFARAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMRKENYVDIL
                     KQHLKTSVRKLKLGRKWVFQMDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPTYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    8940..9074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8964..8983)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9018..9037
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9317..9333
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9343..9362
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9343..9361
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg
       61 ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct
      121 ctagagtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      181 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      241 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      301 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      361 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      421 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      481 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
      541 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      601 tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
      661 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
      721 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      781 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
      841 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
      901 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
      961 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     1021 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     1081 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     1141 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     1201 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     1261 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     1321 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     1381 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     1441 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1501 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     1561 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     1621 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     1681 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     1741 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     1801 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcat
     1861 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctag aggatggaac
     1921 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
     1981 ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt
     2041 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
     2101 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
     2161 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgaacatttc
     2221 gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
     2281 aaaattacca ataatccaga aaattattat catggattct aaaacggatt accagggatt
     2341 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
     2401 tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaact cctctggatc
     2461 tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca gattctcgca
     2521 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
     2581 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
     2641 agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc aggattacaa
     2701 aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa gcactctgat
     2761 tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac ctctttcgaa
     2821 agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac aaggatatgg
     2881 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     2941 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     3001 gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta tgtccggtta
     3061 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     3121 agacatagct tactgggacg aagacgaaca cttcttcata gttgaccgct tgaagtcttt
     3181 aattaaatac aaaggatacc aggtggcccc cgctgaattg gagtcgatat tgttacaaca
     3241 ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc
     3301 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     3361 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     3421 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     3481 ggccaagaag ggcggaaagt ccaaattgta aaatgtaact gtattcagcg atgacgaaat
     3541 tcttagctat tgtaatactc taggggggat ccactagaat tcactcctca ggtgcaggct
     3601 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     3661 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     3721 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     3781 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     3841 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     3901 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     3961 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     4021 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     4081 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgt ggaaggctac
     4141 tcgaaatgtt tgacccaagt taaacaattt aaaggcaatg ctaccaaata ctaattgagt
     4201 gtatgtaaac ttctgaccca ctgggaatgt gatgaaagaa ataaaagctg aaatgaatca
     4261 ttctctctac tattattctg atatttcaca ttcttaaaat aaagtggtga tcctaactga
     4321 cctaagacag ggaattttta ctaggattaa atgtcaggaa ttgtgaaaaa gtgagtttaa
     4381 atgtatttgg ctaaggtgta tgtaaacttc cgacttcaac tgtataggga tcctctagct
     4441 agagtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt gagggttaat
     4501 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4561 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4621 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4681 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5101 aaccgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5341 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5641 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6121 tccgatcgtt gtcagaagta acttggccgc agtgttatca ctcatggtta tggcagcact
     6181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6301 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6361 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6421 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6481 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6541 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6601 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6661 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     6721 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     6781 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     6841 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     6901 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     6961 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     7021 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     7081 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     7141 attcgagctc ggtacccggg ggcgcgccct accgggtagg ggaggcgctt ttcccaaggc
     7201 agtctggagc atgcgcttta gcggccccgc tgggcacttg gcgctacaca agtggcctct
     7261 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     7321 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     7381 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     7441 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     7501 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     7561 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     7621 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     7681 tccatctaga tccttaatta agtctagagt cgaggaactg aaaaaccaga aagttaactg
     7741 gtaagtttag tctttttgtc ttttatttca ggtcccggat ccggtggtgg tgcaaatcaa
     7801 agaactgctc ctcagtggat gttgccttta cttctaggcc tgtacggaag tgttacttct
     7861 gctctaaaag ctgcggaatt gtacccgcgg ccgatccgac atcatgggaa aatcaaaaga
     7921 aatcagccaa gacctcagaa aaaaaattgt agacctccac aagtctggtt catccttggg
     7981 agcaatttcc aaacgcctgg ccgtaccacg ttcatctgta caaacaatag tacgcaagta
     8041 taaacaccat gggaccacgc agccgtcata ccgctcagga aggagacgcg ttctgtctcc
     8101 tagagatgaa cgtactttgg tgcgaaaagt gcaaatcaat cccagaacag ccgcaaagga
     8161 ccttgtgaag atgctggagg aaacaggtac aaaagtatct atatccacag taaaacgagt
     8221 cctatatcga cataacctga aaggccgctc agcaaggaag aagccactgc tccaaaaccg
     8281 acataagaaa gccagactac ggtttgcaag agcacatggg gacaaagatc gtactttttg
     8341 gagaaatgtc ctctggtctg atgaaacaaa aatagaactg tttggccata atgaccatcg
     8401 ttatgtttgg aggaagaagg gggaggcttg caagccgaag aacaccatcc caaccgtgaa
     8461 gcacgggggt ggcagcatca tgttgtgggg gtgctttgct gcaggaggga ctggtgcact
     8521 tcacaaaata gatggcatca tgaggaagga aaattatgtg gatatattga agcaacatct
     8581 caagacatca gtcaggaagt taaagcttgg tcgcaaatgg gtcttccaaa tggacaatga
     8641 ccccaagcat acttccaaag ttgtggcaaa atggcttaag gacaacaaag tcaaggtatt
     8701 ggagtggcca tcacaaagcc ctgacctcaa tcctatagaa aatttgtggg cagaactgaa
     8761 aaagcgtgtg cgagcaagga ggcctacaaa cctgactcag ttacaccagc tctgtcagga
     8821 ggaatgggcc aaaattcacc caacttattg tgggaagctt gtggaaggct acccgaaacg
     8881 tttgacccaa gttaaacaat ttaaaggcaa tgctaccaaa tactagaatt ggccgcgggg
     8941 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     9001 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     9061 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     9121 tgtgggaggt tttttcggat cctctagagt cgactgttta ttaacgctta caatttccat
     9181 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     9241 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     9301 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     9361 ggcgaattgg agctcggatc cctatacagt tgaagtcgga agtttacata cacttaagtt
     9421 ggagtcatta aaactcgttt ttcaactact ccacaaattt cttgttaaca aacaatagtt
     9481 ttggcaagtc agttaggaca tctactttgt gcatgacaca agtcattttt ccaacaattg
     9541 tttacagaca gattatttca cttataattc actgtatcac aattccagtg ggtcagaagt
     9601 ttacatacac taagttgact gtgcctttaa acagcttgga aaattccaga aaatgatgtc
     9661 atggctttag aagctagatc tggccatcta gagcggccgc gcgcactagt gaattccatg
     9721 gatatcaagc tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa
     9781 gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct
     9841 gc
//
