LOCUS       sequence-42903-e        1247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CeGFP
FEATURES             Location/Qualifiers
     source          1..1247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        219..237
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     219..236
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             323..1036
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(353..381)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     976..996
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    1090..1224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1114..1133)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1168..1187
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg cgatggctgc
       61 caagtacatc ccccattgac gtcaatgggg aggggcaatg acgcaaatgg gcgttccatt
      121 gacgtaaatg ggcggtaggc gtgcctaatg ggaggtctat ataagcaatg ctcgtttagg
      181 gaaccgccat tctgcctggg gacgtcggag caagcttgat ttaggtgaca ctatagaata
      241 caagctactt gttctttttg caggatccac tagtggcgcg ccattaatta aaggccggcc
      301 agcgatcgcc ggacatcgat tgatgagtaa aggagaagaa cttttcactg gagttgtccc
      361 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      421 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      481 acctgttcca tggccaacac ttgtcactac tctcacttat ggtgttcaat gcttttcaag
      541 atacccagat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
      601 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      661 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      721 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      781 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      841 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      901 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
      961 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     1021 ggatgaacta tacaaataag aattcaaggc ctctcgagcc tctagaacta tagtgagtcg
     1081 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     1141 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     1201 attataagct gcaataaaca agttaacaac aacaattgca ttcattt
//
