LOCUS       sequence-429031-         553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of cysteine-free variant of the E. coli 
            ribonuclease HI gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Bhattacharya S, Varney KM, Dahmane T, Johnson BA, Weber DJ, Palmer 
            AG 3rd
  TITLE     Deuterium spin relaxation of fractionally deuterated ribonuclease H 
            using paired 475 and 950 MHz NMR spectrometers.
  JOURNAL   J Biomol NMR. 2024 Jun 10. doi: 10.1007/s10858-024-00443-w.
  PUBMED    38856928
REFERENCE   2  (bases 1 to 553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomol 
            NMR. 2024 Jun 10. doi: 10.1007/s10858-024-00443-w."
COMMENT     Sequence Label: pMCSG7-10xHis-ecRNHI*
FEATURES             Location/Qualifiers
     source          1..553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..78
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcatcatc accatcaccn tcaccaccat cactcttctg gtgtagatct gggtaccgag
       61 aacctgtact tccaatccaa tgccatgctt aaacaggtag aaattttcac cgatggttcg
      121 gctctgggca atccagggcc cgggggttac ggcgccattt tacgctatcg cggccgcgag
      181 aaaacctttt ctgcaggcta cacgcgtacc accaacaacc gtatggagct catggccgct
      241 attgtcgcgc tggaggcgtt aaaagaacat gccgaagtca ttttgtcgac cgacagccag
      301 tatgtccgcc aaggtatcac ccagtggatc cataactgga aaaaacgtgg ctggaaaacc
      361 gcggacaaaa aaccagtaaa aaatgtagat ctctggcaac gtcttgatgc tgccctaggg
      421 cagcatcaaa tcaaatggga atgggttaaa ggccatgccg gtcacccgga aaacgaacgc
      481 gcagatgagc tagcgcgcgc cgcagcgatg aatcccacac tggaagatac agggtatcaa
      541 gttgaagttt gac
//
