LOCUS       sequence-429033-       17015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rosa26 targeting vector, Cre-dependent tdtomato expression. See 
            article for more colors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17015)
  AUTHORS   Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, 
            Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H
  TITLE     A robust and high-throughput Cre reporting and characterization 
            system for the whole mouse brain.
  JOURNAL   Nat Neurosci. 2010 Jan . 13(1):133-40.
  PUBMED    20023653
REFERENCE   2  (bases 1 to 17015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2010 Jan . 13(1):133-40."
COMMENT     Sequence Label: Ai9
FEATURES             Location/Qualifiers
     source          1..17015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1085
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1486..1762
                     /label=chicken beta-actin promoter
     intron          1763..2779
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2703..2725
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2787..2806
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2855..2888
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    2912..2945
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3112..3246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3366..3500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3620..3754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3783..3816
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3891..3900
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3897..5327
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    5393..5981
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5446..5466)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5864..5875)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5984..6000)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6008..6215
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6223..6256
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        6278..6777
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    6797..6830
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      6831..6840
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6837..7640
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6900..6919)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7510..7529
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7650..8111
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     misc_feature    8177..12517
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        12567..13066
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             13093..13665
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          13742..13807
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    13881..14105
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(14167..14187)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14167..14185)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14206..14222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14206..14222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14219..14241)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14230..14246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14254..14260,14261..14278,14279..14284))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14299..14320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14437..14454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14608..15196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14688..14707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15367..16158,16159..16227))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15990..16009
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16228..16332)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      16359..16814
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16446..16465)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16656..16677
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     16940..16962
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16954..16971
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16955..16971
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16981..17000
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16981..16999
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
     1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1561 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggggcg cgccaggcgg
     1621 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
     1681 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     1741 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
     1801 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     1861 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     1921 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     1981 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     2041 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     2101 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     2161 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     2221 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     2281 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     2341 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     2401 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     2461 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     2521 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     2581 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     2641 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     2701 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     2761 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     2821 tggcaaagaa ttgatttgat accgcgggcc ctaagaagtt cctattctct agaaagtata
     2881 ggaacttcgt cgacatttaa atcatttaaa tataacttcg tataatgtat gctatacgaa
     2941 gttattcgcg atgaataaat gaaagcttgc agatctgcga ctctagagga tctgcgactc
     3001 tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     3061 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     3121 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3181 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3241 tggatctgcg actctagagg atcataatca gccataccac atttgtagag gttttacttg
     3301 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3361 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3421 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3481 tatcttatca tgtctggatc tgcgactcta gaggatcata atcagccata ccacatttgt
     3541 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     3601 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3661 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3721 caaactcatc aatgtatctt atcatgtctg gatccccatc aagctgatcc ggaaccctta
     3781 atataacttc gtataatgta tgctatacga agttattagg tccctcgacc tgcagcccaa
     3841 gctagatcga attcggccgg ccttgtacgc gttaagtgca acacgatccc gccaccatgg
     3901 tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct
     3961 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     4021 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     4081 tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg
     4141 attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     4201 acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca
     4261 aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca
     4321 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga
     4381 tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct
     4441 acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca
     4501 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc
     4561 accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct
     4621 cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg
     4681 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     4741 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     4801 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     4861 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     4921 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     4981 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     5041 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     5101 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     5161 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     5221 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     5281 gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaagaa ttgtgttgca
     5341 cttaacgcgt acaaggccgg ccctgcagga attcgatatc aagcttatcg ataatcaacc
     5401 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5461 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5521 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5581 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5641 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5701 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5761 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     5821 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5881 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5941 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     6001 acctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6061 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6121 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6181 gaggattggg aagacaatgg caggcatgct ggggaactag tggtgccagg gcgtgccctt
     6241 gggctccccg ggcgcggcgg ccgcatcgaa ttctaccggg taggggaggc gcttttccca
     6301 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     6361 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     6421 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     6481 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     6541 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     6601 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     6661 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     6721 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     6781 tgcaatcgcc gctagcgaag ttcctattct ctagaaagta taggaacttc gccaccatgg
     6841 gatcggccat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     6901 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     6961 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     7021 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     7081 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     7141 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     7201 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     7261 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     7321 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     7381 ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     7441 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     7501 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     7561 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     7621 ttcttgacga gttcttctga ggggatccgc tgtaagtctg cagaaattga tgatctatta
     7681 aacaataaag atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg
     7741 agaacagagt acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg
     7801 ggattagata aatgcctgct ctttactgaa ggctctttac tattgcttta tgataatgtt
     7861 tcatagttgg atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc
     7921 ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc
     7981 cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg
     8041 agatcagcag cctctgttcc acatacactt cattctcagt attgttttgc caagttctaa
     8101 ttccatcaga aagcttgcag aagatctccc caactggggt aacctttgag ttctctcagt
     8161 tgggggggcg cgccggctag aagatgggcg ggagtcttct gggcaggctt aaaggctaac
     8221 ctggtgtgtg ggcgttgtcc tgcaggggaa ttgaacaggt gtaaaattgg agggacaaga
     8281 cttcccacag attttcggtt ttgtcgggaa gttttttaat aggggcaaat aaggaaaatg
     8341 ggaggatagg tagtcatctg gggttttatg cagcaaaact acaggttatt attgcttgtg
     8401 atccgcctcg gagtattttc catcgaggta gattaaagac atgctcaccc gagttttata
     8461 ctctcctgct tgagatcctt actacagtat gaaattacag tgtcgcgagt tagactatgt
     8521 aagcagaatt ttaatcattt ttaaagagcc cagtacttca tatccatttc tcccgctcct
     8581 tctgcagcct tatcaaaagg tattttagaa cactcatttt agccccattt tcatttatta
     8641 tactggctta tccaacccct agacagagca ttggcatttt ccctttcctg atcttagaag
     8701 tctgatgact catgaaacca gacagattag ttacatacac cacaaatcga ggctgtagct
     8761 ggggcctcaa cactgcagtt cttttataac tccttagtac actttttgtt gatcctttgc
     8821 cttgatcctt aattttcagt gtctatcacc tctcccgtca ggtggtgttc cacatttggg
     8881 cctattctca gtccagggag ttttacaaca atagatgtat tgagaatcca acctaaagct
     8941 taactttcca ctcccatgaa tgcctctctc ctttttctcc atttataaac tgagctatta
     9001 accattaatg gtttccaggt ggatgtctcc tcccccaata ttacctgatg tatcttacat
     9061 attgccaggc tgatatttta agacattaaa aggtatattt cattattgag ccacatggta
     9121 ttgattactg cttactaaaa ttttgtcatt gtacacatct gtaaaaggtg gttccttttg
     9181 gaatgcaaag ttcaggtgtt tgttgtcttt cctgacctaa ggtcttgtga gcttgtattt
     9241 tttctattta agcagtgctt tctcttggac tggcttgact catggcattc tacacgttat
     9301 tgctggtcta aatgtgattt tgccaagctt cttcaggacc tataattttg cttgacttgt
     9361 agccaaacac aagtaaaatg attaagcaac aaatgtattt gtgaagcttg gtttttaggt
     9421 tgttgtgttg tgtgtgcttg tgctctataa taatactatc caggggctgg agaggtggct
     9481 cggagttcaa gagcacagac tgctcttcca gaagtcctga gttcaattcc cagcaaccac
     9541 atggtggctc acaaccatct gtaatgggat ctgatgccct cttctggtgt gtctgaagac
     9601 cacaagtgta ttcacattaa ataaataaat cctccttctt cttctttttt ttttttttaa
     9661 agagaatact gtctccagta gaatttactg aagtaatgaa atactttgtg tttgttccaa
     9721 tatggtagcc aataatcaaa ttactcttta agcactggaa atgttaccaa ggaactaatt
     9781 tttatttgaa gtgtaactgt ggacagagga gccataactg cagacttgtg ggatacagaa
     9841 gaccaatgca gactttaatg tcttttctct tacactaagc aataaagaaa taaaaattga
     9901 acttctagta tcctatttgt ttaaactgct agctttactt aacttttgtg cttcatctat
     9961 acaaagctga aagctaagtc tgcagccatt actaaacatg aaagcaagta atgataattt
    10021 tggatttcaa aaatgtaggg ccagagttta gccagccagt ggtggtgctt gcctttatgc
    10081 ctttaatccc agcactctgg aggcagagac aggcagatct ctgagtttga gcccagcctg
    10141 gtctacacat caagttctat ctaggatagc caggaataca cacagaaacc ctgttgggga
    10201 ggggggctct gagatttcat aaaattataa ttgaagcatt ccctaatgag ccactatgga
    10261 tgtggctaaa tccgtctacc tttctgatga gatttgggta ttattttttc tgtctctgct
    10321 gttggttggg tcttttgaca ctgtgggctt tctttaaagc ctccttcctg ccatgtggtc
    10381 tcttgtttgc tactaacttc ccatggctta aatggcatgg ctttttgcct tctaagggca
    10441 gctgctgaga tttgcagcct gatttccagg gtggggttgg gaaatctttc aaacactaaa
    10501 attgtccttt aatttttttt ttaaaaaatg ggttatataa taaacctcat aaaatagtta
    10561 tgaggagtga ggtggactaa tattaaatga gtccctcccc tataaaagag ctattaaggc
    10621 tttttgtctt atacttaact ttttttttaa atgtggtatc tttagaacca agggtcttag
    10681 agttttagta tacagaaact gttgcatcgc ttaatcagat tttctagttt caaatccaga
    10741 gaatccaaat tcttcacagc caaagtcaaa ttaagaattt ctgactttta atgttaattt
    10801 gcttactgtg aatataaaaa tgatagcttt tcctgaggca gggtctcact atgtatctct
    10861 gcctgatctg caacaagata tgtagactaa agttctgcct gcttttgtct cctgaatact
    10921 aaggttaaaa tgtagtaata cttttggaac ttgcaggtca gattctttta taggggacac
    10981 actaagggag cttgggtgat agttggtaaa atgtgtttca agtgatgaaa acttgaatta
    11041 ttatcaccgc aacctacttt ttaaaaaaaa aagccaggcc tgttagagca tgcttaaggg
    11101 atccctagga cttgctgagc acacaagagt agttacttgg caggctcctg gtgagagcat
    11161 atttcaaaaa acaaggcaga caaccaagaa actacagtta aggttacctg tctttaaacc
    11221 atctgcatat acacagggat attaaaatat tccaaataat atttcattca agttttcccc
    11281 catcaaattg ggacatggat ttctccggtg aataggcaga gttggaaact aaacaaatgt
    11341 tggttttgtg atttgtgaaa ttgttttcaa gtgatagtta aagcccatga gatacagaac
    11401 aaagctgcta tttcgaggtc tcttggttta tactcagaag cacttctttg ggtttccctg
    11461 cactatcctg atcatgtgct aggcctacct taggctgatt gttgttcaaa taaacttaag
    11521 tttcctgtca ggtgatgtca tatgatttca tatatcaagg caaaacatgt tatatatgtt
    11581 aaacatttgt acttaatgtg aaagttaggt ctttgtgggt ttgattttta attttcaaaa
    11641 cctgagctaa ataagtcatt tttacatgtc ttacatttgg tggaattgta taattgtggt
    11701 ttgcaggcaa gactctctga cctagtaacc ctacctatag agcactttgc tgggtcacaa
    11761 gtctaggagt caagcatttc accttgaagt tgagacgttt tgttagtgta tactagttta
    11821 tatgttggag gacatgttta tccagaagat attcaggact atttttgact gggctaagga
    11881 attgattctg attagcactg ttagtgagca ttgagtggcc tttaggcttg aattggagtc
    11941 acttgtatat ctcaaataat gctggccttt tttaaaaagc ccttgttctt tatcaccctg
    12001 ttttctacat aatttttgtt caaagaaata cttgtttgga tctccttttg acaacaatag
    12061 catgttttca agccatattt tttttccttt tttttttttt ttttggtttt tcgagacagg
    12121 gtttctctgt atagccctgg ctgtcctgga actcactttg tagaccaggc tggcctcgaa
    12181 ctcagaaatc cgcctgcctc tgcctcctga gtgccgggat taaaggcgtg caccaccacg
    12241 cctggctaag ttggatattt tgttatataa ctataaccaa tactaactcc actgggtgga
    12301 tttttaattc agtcagtagt cttaagtggt ctttattggc ccttcattaa aatctactgt
    12361 tcactctaac agaggctgtt ggtactagtg gcacttaagc aacttcctac ggatatacta
    12421 gcagattaag ggtcagggat agaaactagt ctagcgtttt gtatacctac cagctttata
    12481 ctaccttgtt ctgatagaaa tatttcagga catctagctt atcgataccg tcgacggtat
    12541 cgataagctt gatatcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
    12601 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
    12661 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
    12721 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
    12781 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
    12841 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
    12901 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
    12961 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
    13021 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcaggtcctc
    13081 gccatggatc ctgatgatgt tgttgattct tctaaatctt ttgtgatgga aaacttttct
    13141 tcgtaccacg ggactaaacc tggttatgta gattccattc aaaaaggtat acaaaagcca
    13201 aaatctggta cacaaggaaa ttatgacgat gattggaaag ggttttatag taccgacaat
    13261 aaatacgacg ctgcgggata ctctgtagat aatgaaaacc cgctctctgg aaaagctgga
    13321 ggcgtggtca aagtgacgta tccaggactg acgaaggttc tcgcactaaa agtggataat
    13381 gccgaaacta ttaagaaaga gttaggttta agtctcactg aaccgttgat ggagcaagtc
    13441 ggaacggaag agtttatcaa aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt
    13501 cccttcgctg aggggagttc tagcgttgaa tatattaata actgggaaca ggcgaaagcg
    13561 ttaagcgtag aacttgagat taattttgaa acccgtggaa aacgtggcca agatgcgatg
    13621 tatgagtata tggctcaagc ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga
    13681 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
    13741 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
    13801 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cagatcctag
    13861 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
    13921 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
    13981 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
    14041 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
    14101 tatggcttct gaggcggaaa gaaccagctg gggctcgagg gggggcccgg tacccagctt
    14161 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
    14221 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    14281 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    14341 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    14401 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    14461 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    14521 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    14581 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    14641 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    14701 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    14761 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    14821 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    14881 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    14941 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    15001 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    15061 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    15121 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    15181 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    15241 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    15301 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    15361 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    15421 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    15481 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    15541 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    15601 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    15661 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    15721 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    15781 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    15841 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    15901 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    15961 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    16021 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    16081 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    16141 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    16201 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    16261 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    16321 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
    16381 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
    16441 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
    16501 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    16561 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    16621 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    16681 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    16741 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    16801 aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    16861 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    16921 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
    16981 taatacgact cactataggg cgaattggag ctcca
//
