LOCUS       sequence-42904-e        5760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NmCherry-ABCB1a
FEATURES             Location/Qualifiers
     source          1..5760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..716
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(154..172)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     422..441
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4780..4914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4804..4823)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4858..4877
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5027..5047)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5027..5045)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5066..5082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5066..5082)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5079..5101)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5090..5106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5114..5120,5121..5138,5139..5144))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5159..5180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5297..5314)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5548..5567)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcgattgat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
       61 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      121 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
      181 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
      241 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
      301 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
      361 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
      421 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
      481 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
      541 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
      601 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
      661 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggaat
      721 tcggagccgg cgcgccatta attaaaggcc ggccaatggt agagggtgac ccacctgctg
      781 tgggtttctc caatggcaat ggaacagtgc agatgaatgg aaatggagct agctttgatg
      841 gaccagccgt gatcgatgtg tcgcctatac atgaggacgc tccttctgct aagcatgcga
      901 gagactctcc tccccctgcc tacagtgatg tcagtggaac gaaggaacag ggaaatggag
      961 gatctgtgga aggagagggt ggagagaagg aagacaagaa agagacaaaa gtagtgggca
     1021 cgtttgaagt gtttcgcttt gcaactggtc tagacgtcgt gttcattctg ctagctcttg
     1081 tgatctctct ctgccatggt gtggccttgc ctgccgttct tcttctattt ggagaagtca
     1141 cagattcttt catcaccaca gcctccgtga acgtgacaga caatttggca gcctttgagg
     1201 aatcggtgga cagtattata actttctcca tctactattc ctacctgggt tgtggagtac
     1261 tcgccctggc ttactttcag gtggtgctgt gggacgtggc agccgagaga cagattcaca
     1321 aagtgaggct tcggtttttc catgccatcc tccgtcaaga gattgcctgg tttgatgtcc
     1381 ataaaggcgg cgaactcaac acaaggcttg cagatgacat tgacaagatc cgcaatggca
     1441 ttggagacaa gcttggcatc atgctgcagt acacggccac gttcgttgct ggcatcacca
     1501 tcggcttcgt gaagagctgg aagctcaccc ttgtcatcct ggcagtctct ctgatactaa
     1561 ttgtacccct ggtgggatct acatccgtga ttatccagcg gatgaccaag caagccctgg
     1621 atgcctatgc taaggcaggg gcgatcgcgg gtgaggtctt ctcaggcata aggactgtgg
     1681 tcgcattcaa cggggaggag aaggagatgg tcaggtactc cagcaacttg gaccaagcta
     1741 aatcaaagac ggtcaaaaag gattttgcca cactacttgc ccagggcttc cttttcttct
     1801 ccatgttctc cagctatgcc attgccttct ggtatggcac agttctgtat ttagataatg
     1861 aaattacacc tggagatatc ctaacgacat tccttgctgt gctctttggt gcgtttgcca
     1921 ttggccaagc gggtccgaac tacagtgact tcacaacagc tcgggcggct gcatctagca
     1981 tctgggaagt catagaccag atccctacca tcgactgctt ctcgaccgac ggaaagaagg
     2041 agaagattac cggacaggtg acctttgaag gggtccattt ctcgtacccg tccagagcat
     2101 cggtcaaggt gctcaatggg atcaacctga aggtggacgt tgggaagacg gtggccatgg
     2161 tcggcagcag tggatgcggg aagagcacct gcatccagct cattcagagg ttctatgatg
     2221 ttgctgaagg cagcatcaaa attgacggca ttgacatccg tgacctcaac gtgagctggc
     2281 ttcgagatca catcggtgtc gtgagccagg aacccatcct gtttgccacc acgatcgagg
     2341 agaacatccg ctatggacgt cttgacgtca cccaggcaga gattgagaaa gccgccgagg
     2401 aagccaacgc ccatgacttc atctccaaac ttccagaggg ttactccaca ttggttggag
     2461 agcgaggagc tcagctgtct ggtggtcaga agcagaggat agccattgcc agggcactgg
     2521 tcaggaaccc tacaatccta ctcctggacg aagccacatc ggctctagac acggagagtg
     2581 aggctaccgt tcaactcgcc ctcgaaaagg cccagcacgg tcgaaccacc ctcgtcatcg
     2641 ctcaccgtct ctcaaccatc ttcaactctg acctcatctg cgctttcaag gagggcgtca
     2701 tctccgagca gggcacccat gaagagctga tgaagaacga gggcggtgtt tatcacacgc
     2761 tcgtcatgaa gcagggcatg aagaaagagg aggaggagaa gaaagagaac gaggttccgc
     2821 tcgatgatga tgatgacgaa gaggatgata gccaaggtga gaaggtctac agagcaggct
     2881 ctggaaagaa gaagctgaca cgagtgctat cacggacaca gtcacagatg tctggagatg
     2941 aagaaaaaca ggatgaagac gagtacgaga aggagcttga gaagcatttc agcatgatgc
     3001 gagtctggaa gctgaacacc ccagagtgtg gtttcatcct cctgggatgc atcggggccg
     3061 caatcaatgg ggccgtccag cctggattcg ccgtcgtctt cagtaaaatt cttggggctt
     3121 acagcattac agatagagct gccctctttg atgaagtgac tatatactgt gttctgtttg
     3181 ctgcccttgg tctactctcc ttactcgcca gtatcataca gggtgttgga tttggcaagt
     3241 cgggaggtga gctgacactg aggcttcgta acatgatgtt tagagctatc ctcagacaga
     3301 acatttcttt ctttgatgac aagaggaatg gaaccggtgc cttgacaact aaattggcga
     3361 cggatgtctc tcttatacaa ggggtgactg gagtaagact tggtatgatc tttgaagtgc
     3421 tcttcaacat cggtgttggt atcgtgatct cgttcgtcta cagctggcag atcgcttgtc
     3481 tccttctctt tgccttcctc cccatcctgt ccttggccgt atgattgggt tggaagatcc
     3541 tccagggtaa ctccatcgga acagccggta gccaggctga ggttgggaag cttgtctccg
     3601 agtgtattga gaacatacgt accgttcagt ccctcaaccg aggacagacc ttccatctga
     3661 agtactgtga actccagaac cctccgtaca aacaagggat caagggagct ttcgccgccg
     3721 gtcttgcgtt cggcttctct caagctacca tcttctttgc atactcagct actttccggc
     3781 ttggtgccca ccttgtgggt actggagacc tcaccttccc tgatgttttc ctgtctttct
     3841 ctgccttgat gtttggtgcc tttggtctcg gtcgcgctgc aggatctgtg ccagacttct
     3901 ccaaggctaa ggtggcaaca ggagagctct tctatttggt cgaccgtagt ccagacattg
     3961 acactttcag cgatgacggg gagaaaccgg cgagctacgg tggatcggtg agcctcaaca
     4021 acgttcggtt ccgctacccg acccgtcctg atgtccctgt cctgaggggg ctgtccgtct
     4081 cggtggaccc tggcgagacc ctggctttgg tcggcagcag cggttgcggc aagagcacca
     4141 ccatccagct gatggagcgc ttctatgacc ctcactcagg gactgtgatg tttgactccc
     4201 acgatgcctc gctgctcaac actcgctggc agcgcgccca ggtgggcctc gtatcccaag
     4261 aaccctgcct cttcgacatg tccatcgcag agaacatcaa gtacggcgac aactctcggg
     4321 aagtctccat cgaagactgc atcgaagcgg ccaaaaagtc caacatccat gactttgtgg
     4381 actctcttcc catgaaatac gacaccaacg ttggatccaa gggcacccag ctgtccggcg
     4441 gtcagaagca gaggatagcc attgccaggg cactggtcag gaaccccaag gtcctgctcc
     4501 tggatgaagc cacatcggcc ttggacactg aaagcgaaag ggttgtacaa gatgccctgg
     4561 atgaagccaa gaagggccgg acctgcatca ccatcgccca tcgcctctca accatccaca
     4621 atgcagagaa gatcgctgtc atccgagaag gaaagctcgc cgagtttggc aagcatgaag
     4681 agctgatggc tatgaagcag caatattatt ccctctacac tgcccagtct atgcagcatt
     4741 aactcgagcc tctagaacta tagtgagtcg tattacgtag atccagacat gataagatac
     4801 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     4861 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     4921 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaattc
     4981 gcggccgcgg cgccaatgca ttgggcccgg tacccagctt ttgttccctt tagtgagggt
     5041 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5101 tcacaattcc acacaacata cgagccggga gcataaagtg taaagcctgg ggtgcctaat
     5161 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5221 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5281 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5341 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5401 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5461 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5521 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5581 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5641 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5701 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
//
