LOCUS       sequence-42905-e        4977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NmCherry-ABCB4a
FEATURES             Location/Qualifiers
     source          1..4977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..716
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(154..172)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     422..441
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4690..4824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4714..4733)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4768..4787
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4938..4958)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4938..4956)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atcgattgat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
       61 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      121 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
      181 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
      241 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
      301 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
      361 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
      421 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
      481 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
      541 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
      601 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
      661 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggaat
      721 tcggagccgg cgcgccatta attaaaggcc ggccaatggt ggtggaaaac ggacatacga
      781 aaggcctcga cgacgacggc actgaggtcg cgttcaagaa tgggaactac ggtgcaacca
      841 atggaaagga caccgcaaac ggaaccatca agggagagga aaagagtgat gagaaggaaa
      901 atgagaaaga ggaagagaag ccacaggtcc ctttcacgag attgtttcgc tatgctacgg
      961 gtctcgacgg cctcttcatg tttatcggct gttttgccgc ggtctgccac ggctgtgcct
     1021 ggccggcgct caacatcgtc ttcggcggtc tcatcgacga gttcgttgat ttcgacaagt
     1081 tgaataccac gaacaccacg gatttcacgg ctactttacc gccgggtttg gatccggcca
     1141 aagagtttga taaccagatg caaatgtacg ccgtcatatt cacttacatt ggcataggtg
     1201 tcatggtgat ggcatacctg cagtcatcga tgtggactct tgccggcgag aggcagatct
     1261 ataagattcg tcaggccttc ttcaacgcca tcttgcatca agaaatccaa tggttcgatg
     1321 tacacaagag tggtgaactc acgtccaggt tggccgacga catggagaga gtgaaagatg
     1381 gactcggaga caagatcgct ctctgcctgc aatctctctc tctattcttg gctggttttg
     1441 gtatagcctt ttggaagagt tgggaattga ccctcgttct tctatccacg acccctctac
     1501 tagcagcagc tgggggcttc atggcttatt ttctgacatc cttcgcaaag ctagagcagg
     1561 agtcgtacgc ccaagccggg agtgtcgcgg aggaggtttt gtcttgcgtc aggactgtca
     1621 tagcatttgg aggagaacag aaggaggtca ctaggtacga gaaggagctg aaagaagcca
     1681 gagatgtggg cgtgaagaag ggcgtgactt cgggcgtcgg catgggtatc accatgttca
     1741 tcatgttcgg gtcctatgca cttgctttct ggtatgggcc taaactcgtt gccgatgggc
     1801 gtataactgg tggtgacgtc atgatcgtat tcttcagcgt catgatcggt tcgttctcca
     1861 tcgggaacat ctcgccttcc atgacagcaa tcacggcagc aaggggtgcg gcagtgaccc
     1921 tcttcgacgt catcgatgcc aggccagcta tcgatactcg gtccaagaag gggatcgtac
     1981 cggcagagat gacggggaac atagacttcc aaggggtgga attctcttac cccacccggg
     2041 acgatgtacc ggtgttgaaa ggtgtagacc tgagcattag gaaggggcag acggtggcct
     2101 tggtggggtc cagtggatgt ggcaagtcga ccactatcaa cctgctccta cgattctatg
     2161 aaaagttggg tggcaatatc ctaatcgacg ggcataagat tgaagagctg aacctccact
     2221 ggttgaggag acacatgggt gtggtcagcc aggaaccggt cctcttcaac tgttccatag
     2281 aaaccaacat ctcttacgga agagacggtg tcaccaagga ggaaatcatc aacgctgcca
     2341 agatggccaa cgcccatgac ttcatctcaa aactacccaa gggttatgat acgatggttg
     2401 gtgagcgtgg agcccagctg tctggtggtc agaagcaaag agtagccatc gccagagctc
     2461 tggtcagaaa cccacctatc cttctactgg acgaagccac ctcagccctg gaccgagaga
     2521 gtgagaaggt cgtacaacaa gctctggata aggcttcgga aggtcgcaca accctcgtga
     2581 ttgctcaccg cttgactacc atcaggaatg ctgacgtcat ctacgccttc gaagacgggc
     2641 gggtcgtcga gtttggtgat cacgctgagc tgatgaagag agacggggtc tacaagcagc
     2701 ttgtgacact gcagactctt gatggtgctg gtgaggaatc aacatcatct tcaaaagaag
     2761 tcgttcgaaa agaatccatc aaacgactac catctcgtca aatgtctcgt cagatctcac
     2821 gacaaatgtc caacggcagt ggcaaaatgg aagaaagtgt tgaggtaaag gaagaagttg
     2881 aggaggagga ggtggaggag agaggttatc ttgaaatctt gaagatgaat aaaccggagt
     2941 ggctttacat cgttgtcggc tgcgtttttg ctggaatcct tggagtcgcc atgcctgctt
     3001 tcgctattct tttcagcgag gtcattgcga tcttcagttt gcccgctgac gaattgagag
     3061 aggaatctgt tttctgggcc ctgatgttcc tggccctcgg aggagcattc tttgtgagta
     3121 acagcgtcac cggttactgc ttttcgatct ctggagagga gttgaccctg aggcttcgca
     3181 agaaagcgtt ctggaccatc ctccgtcagg actgtgcata ttttgaccaa ccaagtcaca
     3241 gtactggtgc ccttgctaca aggctatcat cagacgcatc aaatgtcaaa ggggccaccg
     3301 gaatgcgcat cagcaccatc gtgcaggcta tcgtaaccat ggtcgtcgct atcaccattg
     3361 gcttcgtctt cggctggaag ttggctctcc tcatcttcgg ttgcctcccg gtcctggccc
     3421 tctccggtgc tcttgagatg aagatcctcc agggtgggca tgagaaagac gccgccctca
     3481 tcgaggaagc cggcaagatt gccgccgaag caattgagaa cgttcgtact gtagcctctc
     3541 tcaaccttga ggataggatg attgcgaatt atactgagca gctgcaaaac ccctacaggc
     3601 aaggaaagat taacagccag attaatggtc tggcctttgc tgtctcccag gcgatgatct
     3661 tcttcatcta tgcagcatcc ttccgtctgg gaggatactt ggtatccatt ggtgatatga
     3721 cagtagatga ggtcttcaaa gttgtctttg gggttgcttt tgccggcatc tcggtcggtc
     3781 agtcactggc tttcctccca gactacgcca aagctcgcca ctcagcagat cttatgctcc
     3841 acctgttcag catcaagccg ctcattgata attattccac ggatggtgct caaccgcaaa
     3901 aggtggatgg taagatcgag tacagtggtc tcaagttctc ctacccaact cgacctgacg
     3961 tcaccgtctt gaaagggctg agtctcacca tcaaaccagg ccagaccgta gccctggtag
     4021 gagagagtgg gtgtggtaag agtactctgg tgtcactcct agaaagattc tatgatcctg
     4081 cacaaggttc tgtggctctc gatgggacgc ctgtaaaaga catcaacatc cagtggctgc
     4141 gagcaaacat ggcgattgtg agccaagaac caatcctctt tgcctgttct atcggagaca
     4201 acatccaata tggtgtcgaa acgccaatgg aacaagcggc tattgagaat gtcgccaaga
     4261 tggccaacat tcatgatttc atcgcttctc ttcctcttgg atatgatact cttgtaggag
     4321 agaaaggagc ccagctgtcc ggtggtcaga agcagagagt agccatcgcc agagctatgg
     4381 ctagaaatcc tcgtatccta ctcctggatg aagctacctc agcacttgat acagagagcg
     4441 aaaaggttgt ccaggcagcc cttgacaatg ccatgcaggg ccgaacatcc atcgtcatcg
     4501 cccaccgtct ctccaccatc cagaacgctg acactatcgc tgtaatcaga gagggagtgg
     4561 tcgtcgagag cggtagccac caggaacttc tccaaagcaa gggacactac tttacgttga
     4621 ccggtggcaa gattgatgtg gaatccgaat agctcgagcc tctagaacta tagtgagtcg
     4681 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     4741 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4801 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     4861 cagggggagg tgtggggagg ttttttaatt cgcggccgcg gcgccaatgc attgggcccg
     4921 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatgg
//
