LOCUS       sequence-429057-        2184 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E 
            mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Yang Y, Batista M, Clarke BR, Agyare-Tabbi MR, Song H, Kuehfuss NM, 
            Le Bas A, Robinson CV, Whitfield C, Stansfeld PJ, Naismith JH, Liu J
  TITLE     Molecular basis for the phosphorylation of bacterial tyrosine kinase
            Wzc.
  JOURNAL   Nat Commun. 2025 Apr 11;16(1):3437. doi: 10.1038/s41467-025-58693-7.
  PUBMED    40210632
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-04-11"; volume: "16(1):3437. doi"; pages: " 
            10.1038/s41467-025-58693-7"
COMMENT     Sequence Label: pBN201
FEATURES             Location/Qualifiers
     source          1..2184
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2164..2181
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgacttcag tcacatctaa gcaatcaacg atactaggat ctgatgaaat agatttaggt
       61 cgcgttatcg gtgagctaat tgatcatcgt aagcttatta tatctataac atcggttttt
      121 accttatttg caatactcta tgcattatta gcgactccta tctatgagac ggatgcgcta
      181 attcaaatag agcagaagca aggtaatgct atactaagca gtctaagtca ggttttacct
      241 gatggccaac cccaatctgc acctgaaaca gctttgctac aatctcgaat gattcttggc
      301 aaaactatag atgatttaaa ccttcagatt caaattgaac aaaaatattt tcctgttatt
      361 ggccgtgggt tagcaagatt aatgggagaa aaaccaggta atattgatat tactcggtta
      421 tatttgcctg atagtgacga tattagtaat aatactccta gtataatatt aacagtaaaa
      481 gataaagaaa attattcgat caatagcgat ggtattcaac tcaatggggt tgtagggacg
      541 ttattaaatg aaaaagggat atcattactg gttaacgaaa tcgatgctaa acctggagat
      601 caatttgtta ttacacagtt gcctcgatta aaggccattt ctgatctttt aaaaagtttt
      661 agcgttgctg atttgggtaa agatacggga atgttaacgt tgactcttac tggagataac
      721 ccaaaaagaa tatctcatat tttggacagt atcagtcaga actatcttgc acagaacatt
      781 gctagacaag ctgcgcagga tgctaaaagt ctcgagtttt taaatcaaca attacccaaa
      841 gttcgtgccg aattagattc tgcggaagac aagttaaatg cttatcgtaa gcaaaaagac
      901 tctgttgact tgaatatgga agctaaatca gtacttgatc aaatagttaa cgttgataat
      961 caactaaatg aattaacctt ccgtgaggca gaagtttcac agctatatac taaggaacat
     1021 cctacttata aggcgttaat ggaaaaacgg caaacacttc aagaagaaaa atctaaactt
     1081 aataagcgcg ttagtagtat gccctcgacc cagcaggagg ttctgcgatt aagtcgagat
     1141 gttgaatctg gtcgtgctgt atatttgcaa ttgctaaatc ggcaacaaga attaaacatt
     1201 gccaaatcaa gtgctattgg caacgttaga attattgata atgctgtaac agatcctaat
     1261 cctgtaagac caaagaagac tatcataatt gtaataggtg ttgttttagg gctaatcgtt
     1321 tcggttgtac tggtattgtt ccaagtgttt ctacgccgtg gtatcgaatc cccagagcaa
     1381 ctcgaagaaa tcggaataaa cgtgtatgca agtattccaa tatctgaatg gcttacaaaa
     1441 aatgctcgtc agtcagggaa agtcagaaaa aatcagtcag acacattgct tgctgttggt
     1501 aatcctgccg atttagctgt tgaggccatt cgtggtttac gcacaagttt gcattttgca
     1561 atgatggaag caaaaaataa tgtcctaatg atatcgggtg ctagtccaag cgcaggtatg
     1621 acttttatta gcagtaactt ggcagccact atagcaatta cagggaaaaa agttttattc
     1681 attgatgctg accttcgtaa gggatatgct cataaaatgt ttggtcataa aaacgataaa
     1741 ggattatctg agtttttatc tggacaagcc gctgctgaaa tgatcattga taaagtagaa
     1801 ggggggggat ttgattacat tggtcgtgga caaattcctc caaacccagc tgaattactt
     1861 atgcatccgc gttttgaaca attattaaat tgggcctcac aaaattatga tttaattata
     1921 atagatacac ctccaatttt ggcagtgaca gatgctgcga ttattggtcg atatgctgga
     1981 acatgtctat tagtcgctcg ctttgaaaaa aatactgtta aagaaattga tgtaagtatg
     2041 aagcggtttg agcaaagtgg tgtggtagtc aaaggctgta tacttaacgg tgttgtaaag
     2101 aaagctagca gctactatag atatggccat aatcattatg gcgagtctga ggaagataaa
     2161 aaacaccacc accaccacca ctga
//
