LOCUS       sequence-42906-e        6125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NmCherry-ABCC1
FEATURES             Location/Qualifiers
     source          1..6125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..722
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(160..178)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     428..447
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5533..5667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5557..5576)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5611..5630
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5780..5800)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5780..5798)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5819..5835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5819..5835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5832..5854)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5843..5859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5867..5873,5874..5891,5892..5897))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5912..5933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6050..6067)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctagtcatcg attgatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
       61 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      121 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      181 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      241 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      301 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      361 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      421 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      481 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
      541 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      601 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      661 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      721 aggaattcgg agccggcgcg ccattaatta aaggccggcc aatggcacca aacacttcat
      781 gggtggaatt ttgcggttcc gatttatggg acccatcctt actgaatgct tctgtacctg
      841 atgtgacacc gtgtttccaa gaaacagtgt tgtcatggat accatcagct tttcttgttc
      901 ttatcactcc tttctatctc ctcttcatga aacacaatga cagaggattc attcctatat
      961 ctaaactgca caaggctaaa ttgagtcttg ggttgctgct cattctgcta ccagtattag
     1021 atgtgtgcaa agggatatca gatatgatgc agactgatgg agacttgcca gccgtatact
     1081 tagtcactcc agcagtatta gcaataacta tggttgtagc cttgatcctc atccatgtag
     1141 agagaatgaa aggccagcaa tcctctggtg tgctctttat attctatctt atcagtattc
     1201 tatgtgcagt agcagagttt cattcaaagg ctatcaatgc caaggatcaa ggctccgagg
     1261 atgcattccg ctatgtgacc ttttaccttt acttcttcct tctgattggt cagttagtgt
     1321 tagtcacctt tgcagatcag atgccatata actcgccaga tattgccgaa actaatcctt
     1381 gccctgaagc tagagcctct ttcttgtctg tcatcacatt ctggtggttt acaagcatgg
     1441 tgatcaaggg atataagaag agtctagaga aagctgatct gtggtctcta ctcaccagag
     1501 ataaagcttc tagagtcgtc ccagaatttg aaagtagctg gttaaaagaa gtgcataggg
     1561 tgaatcaatc caagcacagt gcatactctc aagcctcgct gaatcaccct catggtgatg
     1621 gaggtcagaa gtatgctatg acagaactcg atccagatgg gaaatcccct aatggtatca
     1681 acaccaaggt ctaccgccca cctactactg atccatcatc taaaggcaat attgatggtt
     1741 acactcaggc cctagccgta ccacaggtgg agtttataga tagtagcagt aaagcatcgt
     1801 tgtttagagc tgtagctatg aggtacggag agaaattctt ggttgctgtt ctgctcaagt
     1861 tcatccatga ctgcttactc tttgtcaacc cacagatact cagattactg atcaacttca
     1921 cagaagacca tactatatgg caatggaggg gattcttcta cgcaggggtc atgctcatga
     1981 tatcaatgtt caactctact ctattacatc agtactttca tcgttgtttc atcgttggca
     2041 tgcatctcag atccgctatc attggtgtgg tctatagaaa gtctttacaa ctctctagtg
     2101 cagccaggaa aggagctacc gttggtgaga tagtcaacct gatgtcagtt gatgctcagc
     2161 gtttcatgga tctctgtacc tatctcaaca tgttatggtc aggtccattt cagatctcag
     2221 tagctctgta cttcctatgg cagacgttag gtccgtcagt cttggctggg ttaggtgtca
     2281 tgattctact cataccgctc aatgcattgg tagctacgca agcaagaaaa ctacaagtga
     2341 aacagatgca atacaaagat gctagaatca aactgatgag tgaggtgctg agtggcatca
     2401 aggtgttgaa gctgtatgca tgggaggagt ctttccaatc taagattctt accatcagag
     2461 acaaggaact gaaagtctta cgtctagctg cttacctcaa tgccttcacc tcattcacat
     2521 ggacctgtgc acctgtcctc gtttctgtga cgacgtttgc agtgtatgtg atatcagatg
     2581 agaacaacat tcttgatgca gagaaagctt ttgtttcaat cgctctcttt aatatcctgc
     2641 gcttcccact cagtatcatg ccaaatctta tctcaaatat ggtgcagacc agtgtatcac
     2701 tgaagcgttt ggagaagttc ttgaagaatg aacagctaga ccctcagaat gtagaccatt
     2761 tcaacatgcc aggtcactcg ataactgtag atagtggaca tttcacttgg gatagagagg
     2821 agaaaacaac tcttacaaac atcaatctgg acatcaagca aggatctcta gtagcggttg
     2881 taggacaggt aggatgcggg aagtcatcac ttctatctgc gttgctgggg gagatggaga
     2941 aagtagatgg gaaagtcttt gtgcagggtt ccgttgccta tgttcctcag caagcttgga
     3001 tacagaatgc aactctgaga tcaaacattg ttttcagtgg agatcttcat gttactaagt
     3061 acaagcatgt gatacagtca tgtgccttgg ctcgtgatct tgtggtgtta ccaggtggag
     3121 acatgacaga gattggtgaa aagggtatca acctgagtgg tggtcagaag cagagggtca
     3181 gtctggccag agctgtgtat gctaacactg acctctacct gctggacgat cctctgagtg
     3241 ctgtggatgc tcatgtagcc aagcatatct ttggacatgt cataggacct caaggtcttc
     3301 tcaagaacaa gactcggatc ctggtaactc atgggattag cttcctacct caggtcgatc
     3361 agatcattgt tatgatagat ggctccgttt ctgagatcgg ctcttatcag gatctcttgg
     3421 atcagaatgg tgcgtttgca gagtttcttc ggaactacag ccaagatgca gatgagaagg
     3481 aagatgagga cgaggaagag gaggaggagg aagaagatat accagtcaat atatcattgg
     3541 aagatctaac agaagacaga gagccaacca gaaacctaga accactcaga ggaaaagcta
     3601 ggaaactaag taaaggcata gaggctatat cacagtatga tatcttcaac acaccgagta
     3661 agaagacgga ccatgtaatg aagacggagg cctatgatga ggttgtgatg ggggatacag
     3721 aggagaagat aatacgagct gagaaggcaa agctgggcaa tgtcaagttg acagtcttct
     3781 gggcctacat ccgttccatc ggtgtatttc tctccactgt cattgtcgcc ctctacatgc
     3841 tcttcaacgt cacctccgta gcaggtaacc tatggctaag ccgctggagt aatgagccct
     3901 tggttaacgg aacacaacca gactctgtga gggatagata tctcactgtc tacgcattgc
     3961 tcggactcac acagggcatg tggatatatt gtggagatat gttactgtac accggcgctg
     4021 tcagggctac ctctcacatg catcatacca tggttgtaaa gtgtctccgg tatccgatga
     4081 cattctttga gacatcgttg aaaggtcaga tgttgaaccg gtttggtaag gatgtaaacg
     4141 agatggatac gaagatgggt gacaatatga ggaccatgtt actagtagca accaagtaca
     4201 tcaggactat agcagtcatc tgtgctgtac ttcctctctt caccatcatc attctacctc
     4261 taagtctggt tttcatttat atgcagcggt tctacatctg tacctcccgg cagttaaaaa
     4321 gacttgagtc agtgtcacga tcacccatct attcacattt ctctgagacg atcgtcggta
     4381 ccagcaccat cagagcatat caacgtcagc aagacttcat caaacacaat gagcagctga
     4441 ttgataacaa tcatatcaca tactacccta atatcatctc aaacagatgg cttgccttga
     4501 gactagagtg tgttgggaac tgcatcgtat tctttgcggc tctctttgca gtgattggtc
     4561 gatccaatct tagctcgggc atcgtaggat tgtccatcag ctatgccctg cagattaccc
     4621 agacattgaa ttggatggtg cgaatgacca gtgagcttga aaccaatatt gtttctgtgg
     4681 agagagtcaa ggaatattct gaagcaccga cagaggcagc tgctatagta gcagacaaca
     4741 gacctgcaga gtcatggcct gatgaaggca aagtagagtt tatcaactac agtactcgtt
     4801 atagagaagg gcttgacctt gtcatcaaag gaatcaattt tactgtcaag cctggtgaga
     4861 aggttggtgt tgttggaagg acaggagcag ggaaatcttc tctgaccatg gctctcttca
     4921 gaatcattga acctgctgaa ggtcacattg agattgatgg tgtggacatc tcattgattg
     4981 gtctcaaaga tctaaggtct aagatcacca ttatacctca ggatcctgtc ctgtttgctg
     5041 gacctctacg tatgaatctg gatccatttg agagctatac tgatgatgag gtctggctag
     5101 ctcttagact gtctcatcta gcaacgttca tctctagtct tcctgaggga ttgcagtatg
     5161 agtgtgcaga gggaggagaa aacctcagtg ttggtcagcg gcagctgatc tgccttgcga
     5221 gggcgctgtt gaggaagagc aagatcttgg tcttagacga agcgactgca gcagtggatc
     5281 tagagacgga tgatctcatc cagactacaa tacgaacaga gttcgcagaa tgtacagtca
     5341 tcacaatagc acataggatc aacactatca tggatagtac aagaatcttg gtgatggatg
     5401 gaggagagat agcagagttt gacacaccca ccgagctact aaccagtgga gggatcttct
     5461 acactatggc caaagatgca ggacttacct catagctcga gcctctagaa ctatagtgag
     5521 tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca aaccacaact
     5581 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     5641 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     5701 gttcaggggg aggtgtggga ggttttttaa ttcgcggccg cggcgccaat gcattgggcc
     5761 cggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     5821 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5881 ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5941 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6001 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6061 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6121 tacgg
//
