LOCUS       sequence-429069-        9901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene 
            under control of TetON promoter. This lentiviral vector is used to 
            generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Ho SM, Hartley BJ, Tcw J, Beaumont M, Stafford K, Slesinger PA, 
            Brennand KJ
  TITLE     Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural
            progenitor cells.
  JOURNAL   Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019.
  PUBMED    26626326
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019."
COMMENT     Sequence Label: pLV-TetO-hNGN2-Puro
FEATURES             Location/Qualifiers
     source          1..9901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2377..2395,2396..2418,2419..2437,2438..2460,2461..2479,
                     2480..2502,2503..2521,2522..2544,2545..2563,2564..2586,
                     2587..2605,2606..2628,2629..2647)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2680..2718
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2715..2739
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3655..3711
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3724..4317
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="EYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVT
                     ELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQ
                     QQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAP
                     RNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4214..4234
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4356..4944
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4409..4429)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4827..4838)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4947..4963)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4948..4964)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5469..5649
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5675..5692)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5681..5905
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5951..6379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6038..6057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6248..6269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6388..6408)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6393..6722
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6573..6708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6635..6654
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6770..6817
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6836..7210
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7340..7461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7377..7396)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7431..7450
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7510..7526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7510..7526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7523..7545)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7534..7550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7558..7564,7565..7582,7583..7588))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7603..7624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7741..7758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7912..8500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7992..8011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8671..9462,9463..9531))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9294..9313
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9532..9636)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9711..9730)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttag atctcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2581 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2641 atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag
     2701 gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     2761 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattcgcc
     2821 accatgttcg tcaaatccga gaccttggag ttgaaggagg aagaggacgt gttagtgctg
     2881 ctcggatcgg cctcccccgc cttggcggcc ctgaccccgc tgtcatccag cgccgacgaa
     2941 gaagaggagg aggagccggg cgcgtcaggc ggggcgcgtc ggcagcgcgg ggctgaggcc
     3001 gggcaggggg cgcggggcgg cgtggctgcg ggtgcggagg gctgccggcc cgcacggctg
     3061 ctgggtctgg tacacgattg caaacggcgc ccttcccggg cgcgggccgt ctcccgaggc
     3121 gccaagacgg ccgagacggt gcagcgcatc aagaagaccc gtagactgaa ggccaacaac
     3181 cgcgagcgaa accgcatgca caacctcaac gcggcactgg acgcgctgcg cgaggtgctc
     3241 cccacgttcc ccgaggacgc caagctcacc aagatcgaga ccctgcgctt cgcccacaac
     3301 tacatctggg cactcaccga gaccctgcgc ctggcggatc actgcggggg cggcggcggg
     3361 ggcctgccgg gggcgctctt ctccgaggca gtgttgctga gcccgggagg agccagcgcc
     3421 gccctgagca gcagcggaga cagcccctcg cccgcctcca cgtggagttg caccaacagc
     3481 cccgcgccgt cctcctccgt gtcctccaat tccacctccc cctacagctg cactttatcg
     3541 cccgccagcc cggccgggtc agacatggac tattggcagc ccccacctcc cgacaagcac
     3601 cgctatgcac ctcacctccc catagccagg gattgtatcg aattcggatc tggcgccacg
     3661 aatttctcgc tactcaagca ggccggtgat gtcgaggaaa accctggtcc tatggtgtct
     3721 agagagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     3781 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     3841 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     3901 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     3961 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     4021 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     4081 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     4141 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     4201 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     4261 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatta
     4321 attaagctag ctaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     4381 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4441 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4501 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4561 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4621 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4681 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4741 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4801 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4861 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4921 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg
     4981 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     5041 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     5101 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5161 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     5221 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     5281 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     5341 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     5401 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     5461 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5521 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5581 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5641 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     5701 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5761 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5821 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     5881 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     5941 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6001 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6061 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6121 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6181 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6241 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6301 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6361 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     6421 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6481 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6541 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6601 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6661 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6721 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     6781 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     6841 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     6901 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     6961 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     7021 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     7081 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     7141 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     7201 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     7261 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     7321 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     7381 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7441 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     7501 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7561 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7621 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7681 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7741 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7801 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7861 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7921 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7981 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8041 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8101 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8221 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8281 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8341 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8401 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8461 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8521 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8581 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8641 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8701 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8761 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8821 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8881 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8941 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9001 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9061 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9121 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9181 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9241 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9301 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9361 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9421 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9481 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9541 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9601 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9661 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     9721 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     9781 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     9841 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     9901 t
//
