LOCUS       sequence-429080-       10332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for gRNA cloning with selectable mMaroon 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   D'Souza LJ, Wright SH, Bhattacharya D
  TITLE     Genetic evidence that uptake of the fluorescent analog 2NBDG occurs 
            independently of known glucose transporters.
  JOURNAL   PLoS One. 2022 Aug 24;17(8):e0261801. doi: 
            10.1371/journal.pone.0261801. eCollection 2022.
  PUBMED    36001583
REFERENCE   2  (bases 1 to 10332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2022-08-24"; volume: "17(8):e0261801. doi"; pages: " 
            10.1371/journal.pone.0261801. eCollection 2022"
COMMENT     Sequence Label: lentiGuide-mMaroon
FEATURES             Location/Qualifiers
     source          1..10332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        162..410
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     162..182
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     333..352
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2292..2367
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2423..2540
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2679..3857
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2910..3848
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3805..3825
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3864..3873
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3870..4604
                     /codon_start=1
                     /product="bright, rapidly maturing, monomeric far-red 
                     fluorescent protein (Bajar et al., 2016)"
                     /label=mMaroon1
                     /translation="MVSKGEELIKENMHTKLYLTGTVNNHYFECTAEGEGKPYEGTQTN
                     RIKVVRGGPLPFAFDILAPCFMYGSKTFINHPPDIPDYFKQSFPEGFTWERTTVYEDGG
                     TLTATQDTSLQDGCLIYNVQVRGENFPSNGPVMQKKTLGWEASTETLYPADGSLEGRLD
                     WALKLVGGGHLHCRLETTYRSKKPAKNLKMPGVYFIDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     misc_feature    4634..5222
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4687..4707)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5105..5116)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5294..5527
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5605..5726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5642..5661)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5696..5715
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5766..5901
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5921..5940)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5922..5940)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5950..5967)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5950..5966)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5959..5981)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6108..6563
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6195..6214)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6405..6426
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6589..6693
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6694..6762,6763..7554)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6912..6931)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7725..8313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8214..8233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8467..8484
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8601..8622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8637..8642,8643..8660,8661..8667)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8675..8691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8680..8702
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8699..8715
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8699..8715
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8734..8754
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8736..8754
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8782..9008
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9009..9189
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9236..9361
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9854..10087
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10272..10316
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
       61 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
      121 tatcgtttca gacccacctc ccaaccccga ggggacccag agagggccta tttcccatga
      181 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
      241 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
      301 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
      361 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc ggagacggtt
      421 gtaaatgagc acacaaaata cacatgctaa aatattatat tctatgacct ttataaaatc
      481 aaccaaaatc ttctttttaa taactttagt atcaataatt agaattttta tgttcctttt
      541 tgcaaacttt taataaaaat gagcaaaata aaaaaacgct agttttagta actcgcgttg
      601 ttttcttcac ctttaataat agctactcca ccacttgttc ctaagcggtc agctcctgct
      661 tcaatcattt tttgagcatc ttcaaatgtt ctaactccac cagctgcttt aactaaagca
      721 ttgtctttaa caactgactt cattagttta acatcttcaa atgttgcacc tgattttgaa
      781 aatcctgttg atgttttaac aaattctaat ccagcttcaa cagctatttc acaagctttc
      841 atgatttctt cttttgttaa taaacaattt tccataatac atttaacaac atgtgatcca
      901 gctgcttttt ttacagcttt catgtcttct aaaactaatt cataattttt gtcttttaat
      961 gcaccaatat ttaataccat atcaatttct gttgcaccat ctttaattgc ttcagaaact
     1021 tcgaatgctt ttgtagctgt tgtgcatgca cctagaggaa aacctacaac atttgttatt
     1081 cctacatttg tgccttttaa taattcttta caatagcttg ttcaatatga attaacacaa
     1141 actgttgcaa aatcaaattc aattgcttca tcacataatt gtttaatttc agctttcgta
     1201 gcatcttgtt ttaataatgt gtgatctata tatttgttta gtttcatttt ttctcctata
     1261 tattcatttt taattttaat tctttaataa tttcgtctac tttaacttta gcgttttgaa
     1321 cagattcacc aacacctata aaataaattt ttagtttagg ttcagttcca cttgggcgaa
     1381 cagcaaatca tgacttatct tctaaataaa attttagtaa gtcttgtcct ggcatattat
     1441 acattccatc gatgtagtct tcaacattaa caactttaag tccagcaatt tgagttaagg
     1501 gtgttgctct caatgatttc attaatggtt caatttttaa tttcttttct tctggtttaa
     1561 aattcaagtt taaagtgaaa gtgtaatatg cacccatttc tttaaataaa tcttctaaat
     1621 agtctactaa tgttttattt tgttttttat aaaatcaagc agcctctgct attaatatag
     1681 aagcttgtat tccatcttta tctctagctg agtcatcaat tacatatcca taactttctt
     1741 cataagcaaa aacaaaattt aatccgttat cttcttcttt agcaatttct ctacccattc
     1801 atttaaatcc agttaaagtt tttacaatat taactccata tttttcatga gcgattctat
     1861 cacccaaatc acttgttaca aaacttgaat atagagccgg attttttgga atgctattta
     1921 agcgttttag atttgataat tttcaatcaa ttaaaattgg tcctgtttga tttccatcta
     1981 atcttacaaa atgaccatca tgttttattg ccattccaaa tctgtcagca tctgggtcat
     2041 tcataataat aatatctgca tcatgtttaa taccatattc aagcggtatt tttcatgcag
     2101 gatcaaattc tggatttgga tttacaacat ttttaaatgt ttcatcttca aatgcatgct
     2161 cttcaacctc aataacgtta tatcctgatt cacgtaatat ttttggggta aatttagttc
     2221 ctgttccatt aactgcgcta aaaataattt ttaaatcttt tttagcttct tgctcttttt
     2281 tgtacgtctc tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     2341 cttgaaaaag tggcaccgag tcggtgcttt tttaagcttg gcgtaactag atcttgagac
     2401 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2461 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2521 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     2581 ggctcgaggg ggcccgggtg caaagatgga taaagtttta aacagagagg aatctttgca
     2641 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     2701 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg
     2761 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2821 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2881 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2941 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     3001 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     3061 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     3121 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     3181 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     3241 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     3301 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     3361 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     3421 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     3481 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     3541 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     3601 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     3661 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3721 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3781 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3841 catttcaggt gtcgtgacgt acggccacca tggtgagcaa gggcgaggag ctgatcaagg
     3901 agaacatgca caccaagctg tacctgacag gcaccgtgaa caaccactac ttcgaatgca
     3961 ccgccgaagg ggagggcaag ccctacgagg gcacccagac caacaggatc aaggtggtgc
     4021 gcggaggccc cctgccgttc gcattcgaca tcctggcccc ctgctttatg tacgggagca
     4081 agaccttcat taaccatcca cctgacattc ccgattattt taagcagtcc ttccctgagg
     4141 gcttcacatg ggagagaacc accgtttacg aagacggggg cacactcact gctacacagg
     4201 acaccagcct ccaggacggc tgcctgatct acaacgtcca agtcagaggg gagaacttcc
     4261 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccagt accgagaccc
     4321 tgtaccccgc tgacggcagc ctggaaggca gactggactg ggccctgaag ctcgtgggcg
     4381 ggggccacct gcactgcaga ctggaaacca catacagatc caagaaaccc gcaaagaacc
     4441 tcaagatgcc cggcgtctat tttattgaca ggagactgga aagaatcaag gaggccgaca
     4501 atgagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
     4561 aactggggca caaacttaat ggcatggacg agctgtacaa gtaatgaatt aattatgaac
     4621 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4681 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4741 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4801 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4861 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4921 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4981 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     5041 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5101 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5161 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5221 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5281 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     5341 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5401 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5461 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5521 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     5581 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     5641 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5701 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     5761 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5821 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5881 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     5941 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6001 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6061 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6121 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6181 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6241 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6301 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6361 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6421 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6481 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6541 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     6601 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6661 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6721 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6781 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6841 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6901 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6961 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7021 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7081 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7141 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7201 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7261 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7321 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7381 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7441 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7501 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7561 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7621 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7681 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7741 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7801 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7861 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7921 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7981 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8041 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8101 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8161 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8221 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8281 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8341 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8401 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8461 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8521 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     8581 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8641 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8701 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     8761 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     8821 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     8881 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     8941 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     9001 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9061 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9121 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9181 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     9241 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     9301 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     9361 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     9421 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     9481 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     9541 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     9601 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     9661 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     9721 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     9781 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     9841 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     9901 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     9961 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10021 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    10081 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    10141 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    10201 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    10261 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    10321 attggtttaa ca
//
