LOCUS       sequence-429084-        8343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-LC3-RFP-LC3-Delta-G in mammalian cells to measure 
            autophagic flux .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, 
            Kodama A, Ishihara T, Mizushima T, Mizushima N
  TITLE     An Autophagic Flux Probe that Releases an Internal Control.
  JOURNAL   Mol Cell. 2016 Nov 17;64(4):835-849. doi: 
            10.1016/j.molcel.2016.09.037. Epub 2016 Nov 3.
  PUBMED    27818143
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.09.037"; journalName: "Mol Cell"; date: 
            "2016-11-17- 17"; volume: "64"; issue: "4"; pages: "835-849"
COMMENT     Sequence Label: pMRX-IP-GFP-LC3-RFP-LC3-Delta-G
FEATURES             Location/Qualifiers
     source          1..8343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(605..1279)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(605..1279)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(664..687)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(862..881)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1131..1149
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(join(1703..2413,2414..2416,2417..2419))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1703..2413,2414..2416,2417..2419))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1745..1766)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2094..2113
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2353..2374
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2416..2425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2440..2814
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(2486..2502)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(2824..3240)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2840..2856)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2872..2894)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3305..3662
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     enhancer        4021..4400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(4502..4520)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4588..4692
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4693..4761,4762..5553)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4911..4930)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5717..6305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6206..6225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6459..6476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             6606..7075
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(7299..7898)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7382..7402)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7879..7898
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    join(7899..7907,7908..7910,7911..8343,1..142)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8072..8091)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     8301..8318
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 caagaagaca gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga
       61 aaataacata tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt
      121 aggggggggg gagggagagg gggggggggg gcggaattta cgtagcggcc gcggcgcgcc
      181 ggccctcgag gcctgcagga attcccgtac caccacactg ggatccttag aacgtctcct
      241 gggaggcata gaccatgtac aggaagccgt cttcatctct ctcgctctcg tacacttcag
      301 agatgggtgt ggacacactc accatgctgt gcccattcac caggaggaag aaggcttggt
      361 tagcattgag ctgcaggcgc cttctaatta tcttgatgag ttcgctcata ttcacgtgat
      421 caggtacaag gaacttggtc ttgtccagga cgggcagctg cttctcaccc ttgtatcgct
      481 ctataatcac tgggatcttg gtggggtgct gctcccggat gagccggaca tcttccactc
      541 tttgttcgaa gctccggcgc tgtttgaagg tcttctcgga cggcatggaa ttcaattcat
      601 cgatggcgcc ggtggagtgg cggccctcgg cgcgctcgta ctgttccacg atggtgtagt
      661 cctcgttgtg ggaggtgatg tccagcttga tgtcggtctt gtaggcgccg ggcagctgca
      721 cgggcttctt ggccatgtag gtggtcttga cctcggcgtc gtagtggccg ccgtccttca
      781 gcttcagcct catcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg
      841 tggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg
      901 tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca
      961 cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
     1021 aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt
     1081 actggaactg aggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct
     1141 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
     1201 actcgtggcc gttcacggag ccctccatgc gcaccttgaa gcgcatgaac tccttgatga
     1261 cgtcctcgga ggaggccatc ccgaacgtct cctgggaggc atagaccatg tacaggaagc
     1321 cgtcttcatc tctctcgctc tcgtacactt cagagatggg tgtggacaca ctcaccatgc
     1381 tgtgcccatt caccaggagg aagaaggctt ggttagcatt gagctgcagg cgccttctaa
     1441 ttatcttgat gagttcgctc atattcacgt gatcaggtac aaggaacttg gtcttgtcca
     1501 ggacgggcag ctgcttctca cccttgtatc gctctataat cactgggatc ttggtggggt
     1561 gctgctcccg gatgagccgg acatcttcca ctctttgttc gaagctccgg cgctgtttga
     1621 aggtcttctc ggacggcatg gaattcaatt catcgataga tccgccgcca cccgacccac
     1681 caccgcccga gccaccgcca cccttgtaca gctcgtccat gccgagagtg atcccggcgg
     1741 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
     1801 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     1861 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     1921 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     1981 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     2041 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     2101 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     2161 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     2221 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     2281 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     2341 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     2401 cctcgccctt gctcaccatg gtggcggcgg gagatccggc agtctagagg atggtccacc
     2461 cccggggtcg gcagccttca cgtgggcggc gtgtatccaa gctgcgatgc cgtctacttt
     2521 gagggcggtg ggggtggtca gcaggactgt gtaaggtcct ttccagcgag gttctaggtt
     2581 cttagtctgg tgtcggcgga cccacactgt gtcgccgact cggtaagggt gaggtaccac
     2641 cggtcggtcc agttgttctt ggtaggctgc cgccagaggt ctccagactt cgtgctggac
     2701 taagtagaga gcctgtaagt gagcttggag agaggggctg ttagtaactc ttgtcatgtc
     2761 agggtcaggg aagtttacaa ggggcggggg tgccccatat aagatctcat atggccatat
     2821 gggggcgcct agagaaggag tgagggctgg ataaagggag gatcgaggcg gggtcgaacg
     2881 aggaggttca agggggagag acggggcgga tggaggaaga ggaggcggag gcttagggtg
     2941 tacaaagggc ttgacccagg gaggggggtc aaaagccaag gcttcccagg tcacgatgta
     3001 ggggacctgg tctgggtgtc catgcgggcc aggtgaaaag accttgatct taacctgggt
     3061 gatgaggtct cggttaaagg tgccgtctcg cggccatccg acgttaaagg ttggccattc
     3121 tgcagagcag aaggtaaccc aacgtctctt cttgacatct accgactggt tgtgagcgat
     3181 ccgctcgaca tctttccagt gacctaaggt caaacttaag ggagtggtaa cagtctggcc
     3241 cgggcccata ttttcagaca aatacagaaa cacagtcaga cagagacaac acagaacgat
     3301 gctgcagcag acaagacgcg cggcgcggct tcggtcccaa accgaaagca aaaattcaga
     3361 cggaggcggg aactgtttta ggttctcgtc tcctaccaga accacatatc cctcctctaa
     3421 ggggggtgca ccaaagagtc caaaacgatc gggatttttg gactcaggtc gggccacaaa
     3481 aacggccccc gaagtccctg ggacgtctcc cagggttgcg gccgggtgtt ccgaactcgt
     3541 cagttccacc acgggtccgc cagatacaga gctagttagc taactagtac cgacgcaggc
     3601 gcataaaatc agtcatagac actagacaat cggacagaca cagataagtt gctggccagc
     3661 ttacctcccg gtggtgggtc ggtggtccct gggcaggggt ctcccgatcc cggacgagcc
     3721 cccaaatgaa agacccccgc tgacgggtag tcaatcactc agaggagacc ctcccaagga
     3781 acagcgagac cacaagtcgg atgcaactgc aagagggttt attggataca cgggtacccg
     3841 ggcgactcag tcaatcggag gactggcgcg ccgagtgagg ggttgtgggc tcttttattg
     3901 agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa
     3961 taaggcacag ggtcatttca ggtccttggg gcaccctgga aacatctgat ggttctctag
     4021 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     4081 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     4141 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     4201 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     4261 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     4321 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     4381 actagtcaat aatcaatgtc aacatggcgg taatgttgga catgagccaa tataaatgta
     4441 catattatga tatggataca acgtatgcaa tgggccaagc tcctcgagtg aagacgaaag
     4501 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4561 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4621 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4681 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4741 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4801 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4861 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4921 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4981 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5041 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5101 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5161 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5221 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5281 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5341 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5401 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5461 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5521 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5581 ctttagattg atttgcggcc ggccgcaaaa ggatctaggt gaagatcctt tttgataatc
     5641 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5701 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5761 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5821 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5881 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5941 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6001 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6061 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6121 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6181 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6241 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6301 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6361 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6421 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6481 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6541 actatggcca tttaatgtaa atacttaaga aaaaaaacca aattaatttt gatacatgct
     6601 gcatgtgaaa gacccccgct gacgggtagt caatcactca gaggagaccc tcccaaggaa
     6661 cagcgagacc acaagtcgga tgcaactgca agagggttta ttggatacac gggtacccgg
     6721 gcgactcagt caatcggagg actggcgccc cgagtgaggg gttgtgggct cttttattga
     6781 gctcggggag cagaagcgcg cgaacagaag cgagaagcga actgattggt tagttcaaat
     6841 aaggcacagg gtcatttcag gtccttgggg caccctggaa acatctgatg gttctctaga
     6901 aactgctgag ggctggaccg catctgggga ccatctgttc ttggccctga gccggggcag
     6961 gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc tgttcttggc
     7021 cctgagccgg ggcaggaact gcttaccaca gatatcctgt ttggcccata ttcagctgtt
     7081 ccatctgttc ctgaccttga tctgaacttc tctattctca gttatgtatt tttccatgcc
     7141 ttgcaaaatg gcgttactta agctagcttg ccaaacctac aggtggggtc tttcattccc
     7201 ccctttttct ggagactaaa taaaatcttt tattttatcg tcgagccatg gggtcgtgcg
     7261 ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg cttgcgggtc
     7321 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     7381 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     7441 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     7501 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     7561 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     7621 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     7681 accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     7741 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccgggtcgac ggtgtggcgc
     7801 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggcccgggg gacgtcgtcg
     7861 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg ttgtggccat attatcatcg
     7921 tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg cctagacgtt tttttaacct
     7981 cgactaaaca catgtaaagc atgtgcaccg aggccccaga tcagatccca tacaatgggg
     8041 taccttctgg gcatccttca gccccttgtt gaatacgctt gaggagagcc atttgactct
     8101 ttccacaact atccaactca caacgtggca ctggggttgt gccgcctttg caggtgtatc
     8161 ttatacacgt ggcttttggc cgcagaggca cctgtcgcca ggtggggggt tccgctgcct
     8221 gcaaagggtc gctacagacg ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt
     8281 cacgacattc aacagacctt gcattccttt ggcgagaggg gaaagacccc taggaatgct
     8341 cgt
//
