LOCUS       sequence-429087-        8949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for 
            use with the dTAG knock-in system and BRD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi S, Yang A, 
            Leggett AL, Erb MA, Lawlor MA, Souza A, Scott TG, Vittori S, Perry 
            JA, Qi J, Winter GE, Wong KK, Gray NS, Bradner JE
  TITLE     The dTAG system for immediate and target-specific protein 
            degradation.
  JOURNAL   Nat Chem Biol. 2018 May;14(5):431-441. doi: 
            10.1038/s41589-018-0021-8. Epub 2018 Mar 26.
  PUBMED    29581585
REFERENCE   2  (bases 1 to 8949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-018-0021-8"; journalName: "Nat Chem Biol"; date: 
            "2018-05"; volume: "14"; issue: "5"; pages: "431-441"
COMMENT     Sequence Label: pX330A-nBRD4/PITCh
FEATURES             Location/Qualifiers
     source          1..8949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             561..608
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(636..653)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    642..849
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   858..998
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   858..987
                     /label=AAV2 ITR
     rep_origin      1073..1528
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1160..1179)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1370..1391
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1545..1564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1664..1686
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1724..1742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1810..1914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1915..1983,1984..2775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2133..2152)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2946..3534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3435..3454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3596..3844
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3866..3941
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        4039..4287
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4308..4383
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        4480..4765
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        4767..5043
                     /label=chicken beta-actin promoter
     intron          5044..5271
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      5283..5292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5292..5357
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5364..5384
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5409..8949,1..560)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(5409..8949,1..560)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact
       61 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga
      121 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact
      181 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc
      241 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc
      301 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca
      361 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg
      421 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg
      481 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc
      541 tgtctcagct gggaggcgac aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa
      601 agaaaaagta agaattccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
      661 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
      721 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
      781 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca
      841 tgctggggag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
      901 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
      961 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     1021 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     1081 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     1141 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1201 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     1261 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     1321 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     1381 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     1441 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     1501 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     1561 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     1621 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     1681 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     1741 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1801 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1861 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1921 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1981 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2041 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2101 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2161 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2221 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2281 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2341 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2401 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2461 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2521 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2581 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg aagccgcggt
     2641 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2701 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2761 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2821 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2881 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2941 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3001 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3061 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtgaggg
     3601 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     3661 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     3721 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     3781 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
     3841 caccgatgtc tgcggagagc ggcccgtttt agagctagaa atagcaagtt aaaataaggc
     3901 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt ttagagctag
     3961 aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt ctgcagacaa
     4021 atggctctag aggcatgtga gggcctattt cccatgattc cttcatattt gcatatacga
     4081 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     4141 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     4201 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     4261 tatatcttgt ggaaaggacg aaacaccgca tcgtacgcgt acgtgttgtt ttagagctag
     4321 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     4381 tgcttttttg ttttagagct agaaatagca agttaaaata aggctagtcc gtttttagcg
     4441 cgtgcgccaa ttctgcagac aaatggctct agaggtaccc gttacataac ttacggtaaa
     4501 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat
     4561 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4621 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4681 cgcctggcat tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4741 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     4801 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     4861 agcgatgggg gcgggggggg gggggggcgg gcgccggggg ggtgggggcg gggggggggg
     4921 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     4981 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     5041 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     5101 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     5161 cctccgggct gtaattagct gagcaagagg taagggttta agggatggtt ggttggtggg
     5221 gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg
     5281 gtgccaccat ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca
     5341 aagacgatga cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc
     5401 cagcagccga caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg
     5461 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
     5521 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag
     5581 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
     5641 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
     5701 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
     5761 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
     5821 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
     5881 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
     5941 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
     6001 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
     6061 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg
     6121 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg
     6181 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc
     6241 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg
     6301 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg
     6361 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg
     6421 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct
     6481 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca
     6541 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc
     6601 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc
     6661 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa
     6721 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa
     6781 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg
     6841 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg
     6901 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca
     6961 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct
     7021 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag
     7081 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg
     7141 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga
     7201 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata
     7261 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct
     7321 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct
     7381 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa
     7441 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg
     7501 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata
     7561 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc
     7621 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca
     7681 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
     7741 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
     7801 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
     7861 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
     7921 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca
     7981 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
     8041 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
     8101 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga
     8161 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga
     8221 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga
     8281 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca
     8341 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca
     8401 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc
     8461 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct
     8521 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga
     8581 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg
     8641 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg
     8701 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc
     8761 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg
     8821 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga
     8881 gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca
     8941 tcgactttc
//
