LOCUS       sequence-429091-       14030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14030)
  AUTHORS   Tieu V, Sotillo E, Bjelajac JR, Chen C, Malipatlolla M, Guerrero JA,
            Xu P, Quinn PJ, Fisher C, Klysz D, Mackall CL, Qi LS
  TITLE     A versatile CRISPR-Cas13d platform for multiplexed transcriptomic 
            regulation and metabolic engineering in primary human T cells.
  JOURNAL   Cell. 2024 Feb 29;187(5):1278-1295.e20. doi: 
            10.1016/j.cell.2024.01.035. Epub 2024 Feb 21.
  PUBMED    38387457
REFERENCE   2  (bases 1 to 14030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.01.035"; journalName: "Cell"; date: 
            "2024-02-29- 29"; volume: "187"; issue: "5"; pages: "1278-1295.e20"
COMMENT     Sequence Label: pSLQ4428 pHR
            (EF1a-mCherry-P2A-RfxCas13d-2xNLS...
FEATURES             Location/Qualifiers
     source          1..14030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    243..377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(280..299)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     334..353
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             545..1178
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1225..1350
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1847..2080
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2265..2309
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2607..2724
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2849..4027
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3080..4018
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3975..3995
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4071..4080
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4077..4784
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4222..4240)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4490..4509
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4794..4850
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4854..7754
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="MIEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDS
                     IRSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLE
                     KRYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTV
                     YTYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYII
                     NYGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITN
                     ELTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRK
                     DMSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFV
                     INLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPA
                     GRDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAF
                     FKDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDE
                     NGNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQ
                     KKQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGRE
                     NAEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLE
                     EKGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKK
                     AEEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVH
                     AYINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCV
                     PFGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             7764..7784
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7794..7820
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8307..8372
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8438..9026
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8491..8511)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8909..8920)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9029..9045)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9030..9046)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9136..9369
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9394..9412)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9395..9412)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9702..9721)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9935..9954)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10054..10076
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10114..10132)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10200..10304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10305..10373,10374..11165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10523..10542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11336..11924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11825..11844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12078..12095
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12165..12185)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12170..12499
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12350..12485
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          13633..13698
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13828..13848
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
       61 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
      121 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      181 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      241 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      301 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      361 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
      421 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
      481 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
      541 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
      601 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      661 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      721 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      781 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      841 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      901 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      961 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     1021 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1081 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1141 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1201 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1261 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1321 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1381 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1441 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1501 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1561 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1621 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1681 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1741 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1801 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1861 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1921 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1981 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2041 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2101 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2161 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2221 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2281 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2341 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2401 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2461 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2521 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     2581 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2641 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2701 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     2761 ttaattaatg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
     2821 cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2881 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     2941 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     3001 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     3061 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     3121 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     3181 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     3241 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     3301 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     3361 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     3421 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     3481 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     3541 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     3601 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     3661 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     3721 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     3781 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     3841 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     3901 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     3961 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     4021 gtcgtgaacg cgtctgcagg atacaagctt gcggtaccgc gggcgcgccc gccaccatgg
     4081 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     4141 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     4201 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     4261 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     4321 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     4381 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     4441 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     4501 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     4561 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     4621 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     4681 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     4741 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggcagc ggagctacta
     4801 acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggaccc gggatgatcg
     4861 agaaaaagaa gtccttcgct aagggcatgg gcgtgaagtc cacactcgtg tccggctcca
     4921 aagtgtacat gacaaccttc gccgaaggca gcgacgccag gctggaaaag atcgtggagg
     4981 gcgacagcat caggagcgtg aatgagggcg aggccttcag cgctgaaatg gccgataaaa
     5041 acgccggcta taagatcggc aacgccaaat tcagccatcc taagggctac gccgtggtgg
     5101 ctaacaaccc tctgtataca ggacccgtcc agcaggatat gctcggcctg aaggaaactc
     5161 tggaaaagag gtacttcggc gagagcgctg atggcaatga caatatttgt atccaggtga
     5221 tccataacat cctggacatt gaaaaaatcc tcgccgaata cattaccaac gccgcctacg
     5281 ccgtcaacaa tatctccggc ctggataagg acattattgg attcggcaag ttctccacag
     5341 tgtataccta cgacgaattc aaagaccccg agcaccatag ggccgctttc aacaataacg
     5401 ataagctcat caacgccatc aaggcccagt atgacgagtt cgacaacttc ctcgataacc
     5461 ccagactcgg ctatttcggc caggcctttt tcagcaagga gggcagaaat tacatcatca
     5521 attacggcaa cgaatgctat gacattctgg ccctcctgag cggactgaga cactgggtgg
     5581 tccacaacaa cgaagaagag tccaggatct ccaggacctg gctctacaac ctcgataaga
     5641 acctcgacaa cgaatacatc tccaccctca actacctcta cgacaggatc accaatgagc
     5701 tgaccaactc cttctccaag aactccgccg ccaacgtgaa ctatattgcc gaaactctgg
     5761 gaatcaaccc tgccgaattc gccgaacaat atttcagatt cagcattatg aaagagcaga
     5821 aaaacctcgg attcaatatc accaagctca gggaagtgat gctggacagg aaggatatgt
     5881 ccgagatcag gaaaaatcat aaggtgttcg actccatcag gaccaaggtc tacaccatga
     5941 tggactttgt gatttatagg tattacatcg aagaggatgc caaggtggct gccgccaata
     6001 agtccctccc cgataatgag aagtccctga gcgagaagga tatctttgtg attaacctga
     6061 ggggctcctt caacgacgac cagaaggatg ccctctacta cgatgaagct aatagaattt
     6121 ggagaaagct cgaaaatatc atgcacaaca tcaaggaatt taggggaaac aagacaagag
     6181 agtataagaa gaaggacgcc cctagactgc ccagaatcct gcccgctggc cgtgatgttt
     6241 ccgccttcag caaactcatg tatgccctga caatgttcct ggatggcaag gagatcaacg
     6301 acctcctgac caccctgatt aataaattcg ataacatcca gagcttcctg aaggtgatgc
     6361 ctctcatcgg agtcaacgct aagttcgtgg aggaatacgc ctttttcaaa gactccgcca
     6421 agatcgccga tgagctgagg ctgatcaagt ccttcgctag aatgggagaa cctattgccg
     6481 atgccaggag ggccatgtat atcgacgcca tccgtatttt aggaaccaac ctgtcctatg
     6541 atgagctcaa ggccctcgcc gacacctttt ccctggacga gaacggaaac aagctcaaga
     6601 aaggcaagca cggcatgaga aatttcatta ttaataacgt gatcagcaat aaaaggttcc
     6661 actacctgat cagatacggt gatcctgccc acctccatga gatcgccaaa aacgaggccg
     6721 tggtgaagtt cgtgctcggc aggatcgctg acatccagaa aaaacagggc cagaacggca
     6781 agaaccagat cgacaggtac tacgaaactt gtatcggaaa ggataagggc aagagcgtga
     6841 gcgaaaaggt ggacgctctc acaaagatca tcaccggaat gaactacgac caattcgaca
     6901 agaaaaggag cgtcattgag gacaccggca gggaaaacgc cgagagggag aagtttaaaa
     6961 agatcatcag cctgtacctc accgtgatct accacatcct caagaatatt gtcaatatca
     7021 acgccaggta cgtcatcgga ttccattgcg tcgagcgtga tgctcaactg tacaaggaga
     7081 aaggctacga catcaatctc aagaaactgg aagagaaggg attcagctcc gtcaccaagc
     7141 tctgcgctgg cattgatgaa actgcccccg ataagagaaa ggacgtggaa aaggagatgg
     7201 ctgaaagagc caaggagagc attgacagcc tcgagagcgc caaccccaag ctgtatgcca
     7261 attacatcaa atacagcgac gagaagaaag ccgaggagtt caccaggcag attaacaggg
     7321 agaaggccaa aaccgccctg aacgcctacc tgaggaacac caagtggaat gtgatcatca
     7381 gggaggacct cctgagaatt gacaacaaga catgtaccct gttcaggaac aaggccgtcc
     7441 atctggaagt ggccaggtat gtccacgcct atatcaacga cattgccgag gtcaattcct
     7501 acttccaact gtaccattac atcatgcaga gaattatcat gaatgagagg tacgagaaaa
     7561 gcagcggaaa ggtgtccgag tacttcgacg ctgtgaatga cgagaagaag tacaacgata
     7621 ggctcctgaa actgctgtgt gtgcctttcg gctactgtat ccccaggttt aagaacctga
     7681 gcatcgaggc cctgttcgat aggaacgagg ccgccaagtt cgacaaggag aaaaagaaag
     7741 tgtccggcaa ttccactagt gctcccaaga aaaagcgcaa ggtaggtgga agcccagcag
     7801 ctaaaagagt taaattggat ggatccatat cactgatcgc tgcactcgcg gttgattatg
     7861 taataggcat ggaaaacgcg atgccctgga acctgccagc agacctcgca tggtttaagc
     7921 ggaacactct gaataagcca gtcattatgg gccgacatac gtgggaaagc attggacgcc
     7981 cactgccagg aaggaagaat ataatcctgt catcacagcc gtccacggac gatagagtca
     8041 cttgggtaaa atccgttgac gaagccatag ctgcctgtgg tgatgtgcct gagataatgg
     8101 taataggagg agggcgggta atcgaacaat ttctgcctaa agcccagaag ctgtacctta
     8161 cccatattga cgcggaagta gagggcgaca cacatttccc agattatgag ccagatgatt
     8221 gggagagcgt tttctccgaa tttcatgatg cagacgccca gaatagccac agctactgct
     8281 ttgaaatatt ggagcgccga ggatccgatt ataaggatca cgatggagat tacaaggacc
     8341 acgacataga ctacaaagat gatgacgaca agtaacgtac ggccgcgact ctagagtcga
     8401 cctgcaggca tgcaagcttg atatcaagct tatcgataat caacctctgg attacaaaat
     8461 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     8521 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     8581 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     8641 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     8701 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8761 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8821 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     8881 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8941 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     9001 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgaggga attaattcga
     9061 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9121 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     9181 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9241 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9301 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9361 tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg
     9421 tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc
     9481 taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt
     9541 cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga
     9601 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat
     9661 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     9721 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     9781 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     9841 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     9901 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9961 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
    10021 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
    10081 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
    10141 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
    10201 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10261 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10321 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10381 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    10441 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10501 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    10561 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10621 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10681 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10741 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10801 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10861 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10921 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10981 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    11041 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    11101 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    11161 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11221 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    11281 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    11341 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    11401 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    11461 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    11521 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    11581 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11641 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11701 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11761 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11821 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    11881 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11941 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    12001 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    12061 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    12121 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
    12181 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    12241 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    12301 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
    12361 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    12421 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    12481 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
    12541 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
    12601 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
    12661 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
    12721 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
    12781 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
    12841 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
    12901 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
    12961 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
    13021 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
    13081 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
    13141 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
    13201 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
    13261 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
    13321 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
    13381 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
    13441 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
    13501 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
    13561 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
    13621 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
    13681 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
    13741 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
    13801 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
    13861 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
    13921 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
    13981 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
//
