LOCUS       sequence-429094-        7385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human codon-optimized T7 polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Yun T, Park A, Hill TE, Pernet O, Beaty SM, Juelich TL, Smith JK, 
            Zhang L, Wang YE, Vigant F, Gao J, Wu P, Lee B, Freiberg AN
  TITLE     Efficient reverse genetics reveals genetic determinants of budding 
            and fusogenic differences between Nipah and Hendra viruses and 
            enables real-time monitoring of viral spread in small animal models 
            of henipavirus infection.
  JOURNAL   J Virol. 2015 Jan 15;89(2):1242-53. doi: 10.1128/JVI.02583-14. Epub 
            2014 Nov 12.
  PUBMED    25392218
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02583-14"; journalName: "J Virol"; date: "2015-01-15- 
            15"; volume: "89"; issue: "2"; pages: "1242-53"
COMMENT     Sequence Label: T7opt in pCAGGS
FEATURES             Location/Qualifiers
     source          1..7385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     243..265
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     327..346
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3046..3065)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3111..3166
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3165..3184)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3527..3543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3527..3543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3540..3562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3551..3567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3575..3581,3582..3599,3600..3605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3620..3641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3700..3895
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3746..3881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3901..4035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3938..3957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3992..4011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4103..4120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4274..4862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4354..4373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5033..5824,5825..5893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5656..5675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5894..5998)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6029..6408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6410..6687
                     /label=chicken beta-actin promoter
     intron          join(6688..7385,1..319)
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
ORIGIN
        1 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
       61 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
      121 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
      181 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
      241 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
      301 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
      361 tggcaaagaa ttcgccacca tgaacaccat caatattgcc aagaacgact tttctgatat
      421 cgagctggcc gctattccat tcaatacact ggctgaccac tacggagagc ggctggcccg
      481 cgaacagctg gctctggagc atgaaagcta tgagatggga gaagcccgat tcaggaagat
      541 gtttgagagg cagctgaaag ctggggaagt ggcagacaac gcagccgcta agccactgat
      601 taccacactg ctgcccaaaa tgatcgccag aattaatgat tggttcgagg aagtgaaggc
      661 aaaacgagga aagaggccta ccgccttcca gtttctgcag gagatcaagc cagaagctgt
      721 ggcatacatc accatcaaga ctaccctggc atgcctgaca agcgccgaca acacaactgt
      781 gcaggcagtc gcttccgcaa tcggacgagc tattgaggac gaagcacgct ttgggagaat
      841 ccgggatctg gaggccaagc acttcaagaa aaacgtggag gaacagctga acaagagggt
      901 ggggcatgtc tataagaagg ccttcatgca ggtggtcgag gccgacatgc tgtcaaaggg
      961 actgctggga ggagaggcct ggagctcctg gcacaaagaa gatagcatcc atgtgggagt
     1021 ccgctgcatc gagatgctga ttgaatctac tgggatggtg agtctgcacc gacagaacgc
     1081 cggcgtggtc ggacaggact ctgagacaat cgaactggct cccgagtatg ccgaagctat
     1141 tgcaactaga gccggagctc tggcagggat cagtccaatg ttccagccct gcgtggtccc
     1201 ccctaagcct tggactggca tcaccggggg cggatactgg gctaatggaa ggagaccact
     1261 ggcactggtg cgaacacact ctaagaaagc cctgatgaga tacgaggatg tctatatgcc
     1321 cgaagtgtat aaggccatca acattgctca gaatacagca tggaaaatta acaagaaagt
     1381 gctggccgtc gctaatgtga tcactaagtg gaaacattgt cccgtggagg acatccctgc
     1441 cattgaacgg gaggaactgc ctatgaagcc agaggacatc gatatgaacc ctgaagctct
     1501 gaccgcatgg aagcgcgcag ccgctgcagt gtacagaaag gataaagccc ggaagtcccg
     1561 gcgcatttct ctggagttca tgctggaaca ggccaacaag tttgctaatc acaaagcaat
     1621 ctggttcccc tacaacatgg actggcgcgg acgagtctat gccgtgagca tgttcaaccc
     1681 tcaggggaat gatatgacaa agggcctgct gactctggct aaggggaaac caattgggaa
     1741 ggagggctac tattggctga aaatccacgg ggccaattgc gctggcgtcg acaaggtgcc
     1801 attccccgag aggatcaagt tcatcgagga aaaccatgaa aatattatgg catgtgccaa
     1861 gtctcccctg gagaacacat ggtgggccga acaggatagt cctttctgct ttctggcctt
     1921 ctgttttgag tacgctggag tgcagcacca tgggctgagt tataattgct ccctgccact
     1981 ggcctttgac ggctcttgta gtggaatcca gcacttctcc gcaatgctga gggatgaggt
     2041 cggaggaaga gcagtgaacc tgctgccatc tgagacagtg caggacatct acggcattgt
     2101 cgccaagaaa gtgaatgaga tcctgcaggc tgacgcaatt aacgggactg ataatgaggt
     2161 ggtcaccgtc acagatgaaa acactggcga gatcagcgaa aaggtgaaac tgggaaccaa
     2221 ggccctggct ggacagtggc tggcatacgg agtcacccgc tcagtgacaa agcgaagcgt
     2281 gatgaccctg gcttatggca gcaaagagtt cggcttcagg cagcaggtgc tggaagacac
     2341 catccagcca gccattgatt ccggaaaggg gctgatgttt acacagccca accaggccgc
     2401 tggctacatg gccaagctga tctgggagtc agtgagcgtc acagtggtcg cagccgtgga
     2461 agctatgaat tggctgaagt ccgctgcaaa actgctggcc gctgaggtga aggacaagaa
     2521 aactggcgaa attctgagga aaagatgcgc cgtccactgg gtgacccctg atggattccc
     2581 agtgtggcag gagtataaga aacccatcca gaccagactg aacctgatgt tcctgggcca
     2641 gtttcggctg cagcctacaa tcaacactaa taaggacagt gagattgatg ctcataaaca
     2701 ggaatcaggg attgcaccta attttgtgca cagccaggac ggctcccatc tgcggaagac
     2761 tgtggtctgg gctcacgaga aatacggcat cgaatccttc gcactgattc atgactcttt
     2821 tggaaccatc ccagccgatg cagccaacct gttcaaggct gtccgcgaga ctatggtgga
     2881 cacctacgaa agttgtgatg tgctggccga cttctatgat cagtttgctg accagctgca
     2941 cgagtcacag ctggataaga tgcccgcact gcctgccaaa ggcaacctga atctgagaga
     3001 catcctggag tccgatttcg catttgcctg actcgagcta gcagatcttt ttccctctgc
     3061 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     3121 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     3181 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     3241 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     3301 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     3361 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     3421 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     3481 cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc
     3541 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3601 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3661 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac
     3721 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3781 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3841 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     3901 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3961 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4021 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4081 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4321 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4381 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4441 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4501 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4561 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4621 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4681 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4741 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4801 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4861 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4921 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4981 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5161 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5341 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5641 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5881 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6001 catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta ttaatagtaa
     6061 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     6121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     6181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     6241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     6301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     6361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     6421 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
     6481 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca
     6541 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
     6601 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
     6661 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
     6721 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     6781 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     6841 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
     6901 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
     6961 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     7021 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     7081 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     7141 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     7201 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     7261 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
     7321 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     7381 ctttt
//
