LOCUS       sequence-42910-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Yarar D, Surka MC, Leonard MC, Schmid SL
  TITLE     SNX9 activities are regulated by multiple phosphoinositides through 
            both PX and BAR domains.
  JOURNAL   Traffic. 2008 Jan;9(1):133-46. Epub 2007 Nov 13.
  PUBMED    17988218
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic."; 
            date: "2008-01"; volume: "9(1)"; pages: "133-46. Epub 2007 Nov 13"
COMMENT     Sequence Label: wt 6XHis-SNX9 pET15b
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..94
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 agaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca
       61 tcatcacagc agcggcctgg tgccgcgcgg cagccatatg ctcgaggatc cggccaccaa
      121 ggctcgggtt atgtatgatt ttgctgctga acctggaaat aatgaactga cggttaatga
      181 aggagaaatc atcacaatca caaatccgga tgtaggtgga ggatggctgg aaggaagaaa
      241 catcaaagga gaacgagggc tggttcccac agactacgtt gaaattttac ccagtgatgg
      301 aaaagatcaa ttttcttgtg gaaattcagt ggctgaccaa gccttccttg attctctctc
      361 agccagcaca gctcaggcca gttcgtcggc tgccagcaac aatcaccagg ttggcagtgg
      421 caatgacccc tggtcagcct ggagtgcctc caaatctggg aactgggaaa gctcagaagg
      481 ctggggggcc cagccagagg gggctggagc ccaaagaaac acaaacactc ccaacaactg
      541 ggacactgcc ttcggccacc cccaggccta ccaaggacca gcaactggtg atgatgatga
      601 ctgggatgaa gactgggatg ggcccaaatc ctcttcctac tttaaggatt cagagtcagc
      661 tgatgcaggc ggcgctcagc gaggaaacag tcgtgctagt tcctcatcca tgaaaattcc
      721 ccttaacaaa tttcctggat ttgcgaaacc tggcacggaa cagtatttgt tggccaaaca
      781 actagcaaaa cccaaa
//
