LOCUS       sequence-429106-        9239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  this vector can be used to make modRNA expressing VP64dCas9VP64 and 
            MPH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Haideri T, Lin J, Bao X, Lian XL
  TITLE     MAGIK: A rapid and efficient method to create lineage-specific 
            reporters in human pluripotent stem cells.
  JOURNAL   Stem Cell Reports. 2024 May 14;19(5):744-757. doi: 
            10.1016/j.stemcr.2024.03.005. Epub 2024 Apr 4.
  PUBMED    38579711
REFERENCE   2  (bases 1 to 9239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2024.03.005"; journalName: "Stem Cell Reports"; 
            date: "2024-05-14- 14"; volume: "19"; issue: "5"; pages: "744-757"
COMMENT     Sequence Label: modRNAc1-VP64dCas9VP64-2A-MPH
FEATURES             Location/Qualifiers
     source          1..9239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      110..119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             119..268
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             299..346
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(347..370,371..373,374..2929,2930..2932,2933..4447)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4502..4522
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4547..4696
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4727..4780
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4787..4796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4796..5182
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             5237..5257
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5273..5815
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             5840..6211
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     promoter        complement(6381..6399)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6382..6399)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6417..6433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6417..6433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6430..6452)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6441..6457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6465..6471,6472..6489,6490..6495))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6510..6531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6648..6665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6819..7407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6899..6918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7578..8369,8370..8438))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8201..8220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8439..8543)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8621..9076)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8758..8779)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8970..8989
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9202..9224
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9216..9233
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9217..9233
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataagg cgaattgggc ccgacgtcac atttgcttct gacacaactg
       61 tgttcactag caacctcaaa cagacaccgg atccatggca tgcgaattcg ccaccatgga
      121 cgcattggac gattttgatc tggatatgct gggaagtgac gccctcgatg attttgacct
      181 tgacatgctt ggttcggatg cccttgatga ctttgacctc gacatgctcg gcagtgacgc
      241 ccttgatgat ttcgacctgg acatgctggg tggaggttct ggatgtacgg ccaccatgaa
      301 aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaaggaca agaagtacag
      361 catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
      421 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
      481 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
      541 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
      601 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
      661 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
      721 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
      781 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
      841 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
      901 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
      961 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
     1021 cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg ccctgagcct
     1081 gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct
     1141 gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta
     1201 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct
     1261 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga
     1321 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa
     1381 gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg
     1441 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac
     1501 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga
     1561 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca
     1621 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt
     1681 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac
     1741 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc
     1801 ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa
     1861 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa
     1921 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa
     1981 ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga
     2041 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg
     2101 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
     2161 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
     2221 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
     2281 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
     2341 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
     2401 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
     2461 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
     2521 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
     2581 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
     2641 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
     2701 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
     2761 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
     2821 ggaactggac atcaaccggc tgtccgacta cgatgtggac cacatcgtgc ctcagagctt
     2881 tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaagg cccggggcaa
     2941 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct
     3001 gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg
     3061 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
     3121 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
     3181 tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
     3241 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
     3301 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
     3361 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
     3421 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
     3481 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
     3541 cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt
     3601 gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
     3661 caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg
     3721 ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt
     3781 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
     3841 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
     3901 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
     3961 ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact
     4021 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
     4081 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct
     4141 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa
     4201 tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc
     4261 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta
     4321 ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac
     4381 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
     4441 aggcgacagc gctggaggag gtggaagcgg aggaggagga agcggaggag gaggtagcgg
     4501 acctaagaaa aagaggaagg tggcggccgc tggatccgga cgggctgacg cattggacga
     4561 ttttgatctg gatatgctgg gaagtgacgc cctcgatgat tttgaccttg acatgcttgg
     4621 ttcggatgcc cttgatgact ttgacctcga catgctcggc agtgacgccc ttgatgattt
     4681 cgacctggac atgctgatta actgtacggg cggcgggtcc ggaggagagg gcagaggaag
     4741 tctcctaaca tgcggtgacg tggaggagaa tcctggccca tgtacggcca ccatggcttc
     4801 aaactttact cagttcgtgc tcgtggacaa tggtgggaca ggggatgtga cagtggctcc
     4861 ttctaatttc gctaatgggg tggcagagtg gatcagctcc aactcacgga gccaggccta
     4921 caaggtgaca tgcagcgtca ggcagtctag tgcccagaag agaaagtata ccatcaaggt
     4981 ggaggtcccc aaagtggcta cccagacagt gggcggagtc gaactgcctg tcgccgcttg
     5041 gaggtcctac ctgaacatgg agctcactat cccaattttc gctaccaatt ctgactgtga
     5101 actcatcgtg aaggcaatgc aggggctcct caaagacggt aatcctatcc cttccgccat
     5161 cgccgctaac tcaggtatct acagcgctgg aggaggtgga agcggaggag gaggaagcgg
     5221 aggaggaggt agcggaccta agaaaaagag gaaggtggcg gccgctggat ccccttcagg
     5281 gcagatcagc aaccaggccc tggctctggc ccctagctcc gctccagtgc tggcccagac
     5341 tatggtgccc tctagtgcta tggtgcctct ggcccagcca cctgctccag cccctgtgct
     5401 gaccccagga ccaccccagt cactgagcgc tccagtgccc aagtctacac aggccggcga
     5461 ggggactctg agtgaagctc tgctgcacct gcagttcgac gctgatgagg acctgggagc
     5521 tctgctgggg aacagcaccg atcccggagt gttcacagat ctggcctccg tggacaactc
     5581 tgagtttcag cagctgctga atcagggcgt gtccatgtct catagtacag ccgaaccaat
     5641 gctgatggag taccccgaag ccattacccg gctggtgacc ggcagccagc ggccccccga
     5701 ccccgctcca actcccctgg gaaccagcgg cctgcctaat gggctgtccg gagatgaaga
     5761 cttctcaagc atcgctgata tggactttag tgccctgctg tcacagattt cctctagtgg
     5821 gcagggagga ggtggaagcg gcttcagcgt ggacaccagt gccctgctgg acctgttcag
     5881 cccctcggtg accgtgcccg acatgagcct gcctgacctt gacagcagcc tggccagtat
     5941 ccaagagctc ctgtctcccc aggagccccc caggcctccc gaggcagaga acagcagccc
     6001 ggattcaggg aagcagctgg tgcactacac agcgcagccg ctgttcctgc tggaccccgg
     6061 ctccgtggac accgggagca acgacctgcc ggtgctgttt gagctgggag agggctccta
     6121 cttctccgaa ggggacggct tcgccgagga ccccaccatc tccctgctga caggctcgga
     6181 gcctcccaaa gccaaggacc ccactgtctc ctgaactagt gagctcgctt tcttgctgtc
     6241 caatttctat taaaggttcc tttgttccct aagtccaact actaaactgg gggatattat
     6301 gaagggcctt gagcatctgg attctgccta ataaaaaaca tttattttca ttgcgtcgac
     6361 catatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
     6421 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6481 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6541 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6601 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6661 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6721 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6781 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6841 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6901 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6961 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7021 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7081 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7141 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7201 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7261 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7321 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7381 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7441 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7501 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7561 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7621 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7681 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7741 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7801 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7861 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7921 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7981 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8041 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8101 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8161 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8221 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8281 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8341 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8401 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8461 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8521 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga tgcggtgtga
     8581 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
     8641 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     8701 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     8761 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     8821 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     8881 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     8941 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     9001 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     9061 ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     9121 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     9181 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattg
//
