LOCUS       sequence-429121-       11084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A pAde6 backbone integrating plasmid expressing 
            Pil1-mCherry-SpAtg8(1-115) under the inducible promoter 41nmt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Pan ZQ, Yang YS, Du LL
  TITLE     Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in 
            the Fission Yeast Schizosaccharomyces pombe.
  JOURNAL   Methods Mol Biol. 2025;2862:93-102. doi: 
            10.1007/978-1-0716-4168-2_7.
  PUBMED    39527195
REFERENCE   2  (bases 1 to 11084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2025"; volume: "2862:93-102. doi"; pages: " 
            10.1007/978-1-0716-4168-2_7"
COMMENT     Sequence Label: pDB5230
FEATURES             Location/Qualifiers
     source          1..11084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2707..3162)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2844..2865)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3056..3075
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3288..3310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3302..3319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3303..3319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3329..3348
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3329..3347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5598..6305
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5743..5761)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6011..6030
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      6931..7118
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6986..7010)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            7187..8762
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        7187..7530
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7531..8559
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMRLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVGASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      8565..8762
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8824..8844)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8824..8842)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8863..8879)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8863..8879)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8876..8898)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8887..8903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8911..8917,8918..8935,8936..8941))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8956..8977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9094..9111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9265..9853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9345..9364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10024..10815,10816..10884))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10647..10666
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10885..10989)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11057..11075
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttagc ggccgctaaa cgttgcttta tatatggata
      601 acgacatata catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct
      661 gctttaaagt tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc
      721 aaaactcaaa taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat
      781 tcgttttttc aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca
      841 tcgaatatga gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg
      901 atggtagagg cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct
      961 cctgccaaac aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat
     1021 gcaattgttg aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac
     1081 actgatgcct tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg
     1141 cgatgcattc aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt
     1201 cctgaatttt gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt
     1261 tatccttttg tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt
     1321 catcaaccat ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt
     1381 gaaaagttcg ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga
     1441 aaagtttatg cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta
     1501 tatgcccctg ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat
     1561 gcggtgcgca cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat
     1621 ggtgagacca ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc
     1681 attgaagctt gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct
     1741 ttcagcgaaa tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt
     1801 ttaggtactg atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc
     1861 ggtgcaactt tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac
     1921 gttaccatca tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac
     1981 gtcaaagatc ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta
     2041 agcaagatga aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact
     2101 attgtttcag ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca
     2161 agagggttgc gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt
     2221 gctgcaatga cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga
     2281 gttgactctc ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct
     2341 atcaataata gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc
     2401 tcccttttgg ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag
     2461 gctgataaat tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata
     2521 actgttaaat gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga
     2581 ttgtaaaatg tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt
     2641 gctgcgacgc agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2701 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2761 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2821 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2881 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2941 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3001 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3061 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3121 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     3181 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     3241 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     3301 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     3361 cgggcccgat cagaaaatta tcgccataaa agacagaata agtcatcagc ggttgtttca
     3421 tttcctatat ttttttttta tttttttatt ttttaataag ggaaaattta acgtctaagg
     3481 atacagaaga ttgttagcac attaaagtaa taaaggctta agtagtaagt gccttagcat
     3541 gttattgtat ttcaaaggac ataatctaaa ataataacaa tatcatttct cacaagttat
     3601 tcaattttct tttttttttc taataatatc aagaatgtat tatttgtttg acataagtca
     3661 actaatttat ttaatatgct ggattaatct tgcagacatg taaattaaca agttttagtc
     3721 aaataacgtt gaagtttcaa tgaactcaaa taatttctct ttttttttat ataaccataa
     3781 tctgatttat attttccgca gggatcaact gaagttatga catttggatt ggatcactta
     3841 taaccttggt cgccaaataa tacaaaaatc agcgttataa aacaaagaag gtttttgtta
     3901 agaaattaat cctctttctt gataagaaag ttgaaccgaa attgcagata ctgatatatg
     3961 aaaataatac ccacaatttt gggaatagcg caagcctcaa tttaaacaat aggtgaggac
     4021 acatgataat gacctcaatg attgttagaa gaaaagagcc tcattacaaa atcgaaaaat
     4081 gaatggttgg gtacaagttt ccaaaacatg gtaaagtgga ctttgcgtat gagacgtaaa
     4141 tagaaaaaaa cacttgttat atgttttcta gaattattgt tgtctcttta tggttggatg
     4201 atgcaaaata gtaatttcgg ttagttgctg taaaacacca cgagacaaat agatatggat
     4261 atttattaaa tcaggaaaaa cgtaactctc ggctactgga tggttcagtc acccaacgat
     4321 tactggggag agaaaacagg gcaaaagcaa agcttaaagg aatccgattg tcattcggca
     4381 atgtgcagcg aaactaaaaa ccggataatg gacctgttaa tcgaaacatt gaagataaag
     4441 gaagaggaat cctggcatat catcaattga ataagttgaa ttaattattt caatctcatt
     4501 ctcactttct gacttatagt cgctttgtta aagctagcat gatgaaccgt gcgtactctt
     4561 taagaaacag ccgtgcccct acggcctcac aattgttaaa cccccctcct ccctcctctt
     4621 ccactcgtag tggtcgtttg tttgccccct tgagtcatac catgcgtatc aaccacgctg
     4681 ccaccttcac ccctgacttg gctaagcgtt tggctgtctt ggtcaagatg gaaaagaacg
     4741 tgatgcgcag tatggaggtc accatccgtg gccgtcgtga ttgtgctcgc caactttcct
     4801 actggggtga ggactgcgat gacgatatca gcgacgtcac cgacaaattg ggtgtcttgt
     4861 tttacgagat ggccgagttg gagaactact tgatcgatcg ttatgaccaa taccgtatga
     4921 cgttgaagtc catccgtaac atcgagtcct ctgtccaacc ctctcgtgag aagaagcaaa
     4981 agctcttgga tcaaatctat gctcttaagc ataaggatcc tgaatcccct cgcttggtca
     5041 ccatggagca agagcttgtt cgtgaggagg ctgcctgttt ggttgccgag gctcaacttt
     5101 ccaacaccac ccgtgaaaag ttcaagcaag ccatgacctt caacttggat gccctccacg
     5161 agcatgccga gaagctcaac ctcattgcca cctatggcag acatttgttg aacttgattg
     5221 acgacacccc cgtcactccc ggtgaggctc gtcctgccta cgatggttat gagacttccc
     5281 gccaaatcgt catggatgct gagcacgcct tgagctcttg ggttcccagc caacccgccg
     5341 tcaactttgt caagcctcaa atcgaggatg atgcccaaag tgacgctcgt tcctggaacg
     5401 agtatgaggc tcaaggtgag cccgtccccg ttgagcaagt cacccactta caagacgacg
     5461 ttcaatccga cactagcgag atcattgaga acgagcctgg catgcaccct catgtccatg
     5521 ctgagcgtat gtctcgcatt gcttctgagt ctagcgatgc cgttcctcag caaaccgccg
     5581 tccaggttgc tgctagcatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     5641 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     5701 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     5761 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     5821 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     5881 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     5941 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     6001 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     6061 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     6121 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     6181 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     6241 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     6301 acaagagatc tatgcgttct caattcaagg acgacttttc ttttggtatg tgtcaaaaac
     6361 ttatagaagt tgttttttgg acagcattgt agaatacctg gaactagtag gctaattaat
     6421 aatcagagaa acgaaaaaca gagtctcaaa gaatccggga aaaatatccc gatcgaatac
     6481 ctgtaagtag agtctggtgg tcaaagagaa caggtttcag agcaaaatag tggtgaattg
     6541 aacaacaccc attgtttttg tcatcaaaag tgaatccatt gttacaagtc tgaccttttt
     6601 ctttcaaaaa tacaaattac gttttaaact taactgctaa cttaacaggt tatttgcgag
     6661 aaagttgata aatctgatat tgctgcaata gataaaaaga aatatctcgt tccatctgac
     6721 cttacagttg gtcaatttgt atatgttatt cgcaaacgga taaaactttc tcctgagaag
     6781 gccatcttta ttttcattga tgaaatcttg cccccgacag cggccttgat gtcaacgata
     6841 tacgaagagc ataaaagtga ggacggcttt ttgtatatca cgtattcagg agaaaataca
     6901 tttagatctt aacgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt
     6961 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     7021 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     7081 tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg
     7141 gtaatatgtt tagcttgcct cgtccccgcc gggtcacccg gccagcgaca tggaggccca
     7201 gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc
     7261 gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg
     7321 cacggcgcga agcaaaaatt acggctcctc gctgcggacc tgcgagcagg gaaacgctcc
     7381 cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta
     7441 ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag
     7501 ttctcacatc acatccgaac ataaacaacc atgggtaaaa agcctgaact caccgcgacg
     7561 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcggctctcg
     7621 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     7681 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     7741 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     7801 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     7861 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     7921 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     7981 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     8041 gtcggtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     8101 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     8161 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     8221 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     8281 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     8341 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     8401 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     8461 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     8521 aaccgacgcc ccagcactcg tccgagggca aaggaataat cagtactgac aataaaaaga
     8581 ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt
     8641 aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga
     8701 agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac
     8761 tgctgtcgat tcgatactaa cgccgccatc cacctgcagg taatcacgtg ccagcttttg
     8821 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     8881 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8941 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     9001 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     9061 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9121 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9181 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9241 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9301 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9361 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9421 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9481 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9541 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9601 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9661 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9721 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9781 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9841 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9901 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9961 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10021 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    10081 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10141 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10201 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10261 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10321 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10381 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10441 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10501 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10561 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10621 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10681 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10741 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10801 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10861 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10921 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10981 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    11041 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
