LOCUS       sequence-429128-        6023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of human CALR del52-His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6023)
  TITLE     Masubuchi et al., 2024
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFastBac1/CALR del52-His
FEATURES             Location/Qualifiers
     source          1..6023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..28
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        191..295
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(296..364,365..1156)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(514..533)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1327..1915
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1816..1835
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2069..2086
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2220..2444
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2511..3044)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3214..3219,3220..3236,3237..3242))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3594..3685
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3610..3627
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4989..5006
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5222..5356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5259..5278)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5313..5332
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5385..5550
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      join(5734..6023,1..165)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5821..5840)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
       61 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
      121 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
      181 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      241 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      301 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      361 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      421 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      481 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
      541 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      601 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
      661 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
      721 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
      781 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
      841 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
      901 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
      961 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1021 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1081 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1141 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1201 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1261 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1321 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1381 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1441 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1501 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1561 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1621 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1681 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1741 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1801 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1861 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1921 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1981 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2041 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2101 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagaccagcc
     2161 gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt
     2221 gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc
     2281 ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata
     2341 ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg
     2401 tattaaagag gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat
     2461 ttaccgaaca actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg
     2521 gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga
     2581 gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg
     2641 ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg
     2701 ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga
     2761 acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat
     2821 gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg
     2881 gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg
     2941 tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta
     3001 gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     3061 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
     3121 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     3181 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
     3241 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
     3301 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg
     3361 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg
     3421 ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc
     3481 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag
     3541 gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa tagatcatgg
     3601 agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag
     3661 ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg
     3721 cggatcccgg tccgaagcgc gcggaattcg ccaccatgct gctatccgtg ccgctgctgc
     3781 tcggcctcct cggcctggcc gtcgccgagc ctgccgtcta cttcaaggag cagtttctgg
     3841 acggagacgg gtggacttcc cgctggatcg aatccaaaca caagtcagat tttggcaaat
     3901 tcgttctcag ttccggcaag ttctacggtg acgaggagaa agataaaggt ttgcagacaa
     3961 gccaggatgc acgcttttat gctctgtcgg ccagtttcga gcctttcagc aacaaaggcc
     4021 agacgctggt ggtgcagttc acggtgaaac atgagcagaa catcgactgt gggggcggct
     4081 atgtgaagct gtttcctaat agtttggacc agacagacat gcacggagac tcagaataca
     4141 acatcatgtt tggtcccgac atctgtggcc ctggcaccaa gaaggttcat gtcatcttca
     4201 actacaaggg caagaacgtg ctgatcaaca aggacatccg ttgcaaggat gatgagttta
     4261 cacacctgta cacactgatt gtgcggccag acaacaccta tgaggtgaag attgacaaca
     4321 gccaggtgga gtccggctcc ttggaagacg attgggactt cctgccaccc aagaagataa
     4381 aggatcctga tgcttcaaaa ccggaagact gggatgagcg ggccaagatc gatgatccca
     4441 cagactccaa gcctgaggac tgggacaagc ccgagcatat ccctgaccct gatgctaaga
     4501 agcccgagga ctgggatgaa gagatggacg gagagtggga acccccagtg attcagaacc
     4561 ctgagtacaa gggtgagtgg aagccccggc agatcgacaa cccagattac aagggcactt
     4621 ggatccaccc agaaattgac aaccccgagt attctcccga tcccagtatc tatgcctatg
     4681 ataactttgg cgtgctgggc ctggacctct ggcaggtcaa gtctggcacc atctttgaca
     4741 acttcctcat caccaacgat gaggcatacg ctgaggagtt tggcaacgag acgtggggcg
     4801 taacaaaggc agcagagaaa caaatgaagg acaaacagga cgaggagcag aggacaagga
     4861 ggatgatgag gacaaagatg aggatgagga ggatgaggag gacaaggagg aagatgagga
     4921 ggaagatgtc cccggccagg ccaaggacga gctgtagaga ggcctgcctc cagggctgga
     4981 ctgaggccca tcaccatcac catcactgag aattcaaagg cctacgtcga cgagctcact
     5041 agtcgcggcc gctttcgaat ctagagcctg cagtctcgag gcatgcggta ccaagcttgt
     5101 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5161 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5221 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5281 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5341 ttatcatgtc tggatctgat cactgcttga gcctaggaga tccgaaccag ataagtgaaa
     5401 tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca
     5461 gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct
     5521 cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgttttt
     5581 aatcaaacat cctgccaact ccatgtgaca aaccgtcatc ttcggctact ttttctctgt
     5641 cacagaatga aaatttttct gtcatctctt cgttattaat gtttgtaatt gactgaatat
     5701 caacgcttat ttgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     5761 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5821 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5881 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5941 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6001 ctttgacgtt ggagtccacg ttc
//
