LOCUS       sequence-429159-       15471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The T-DNA backbone of nCas12j2 for genome editing  in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Liu S, Sretenovic S, Fan T, Cheng Y, Li G, Qi A, Tang X, Xu Y, Guo 
            W, Zhong Z, He Y, Liang Y, Han Q, Zheng X, Gu X, Qi Y, Zhang Y
  TITLE     Hypercompact CRISPR-Cas12j2 (CasPhi) enables genome editing, gene 
            activation, and epigenome editing in plants.
  JOURNAL   Plant Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453.
  PUBMED    36127876
REFERENCE   2  (bases 1 to 15471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453."
COMMENT     Sequence Label: pGEL638
FEATURES             Location/Qualifiers
     source          1..15471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    308..448
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(374..393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     524..546
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      782..976
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1042..2115)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2544..3173)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4474..4498
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(4519..4767)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(4778..4825)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(7118..7138)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(7153..9150)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     ncRNA           10710..10746
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     ncRNA           11189..11256
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     protein_bind    complement(11276..11300)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        11657..12333
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12270..12290
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    13423..13597
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13675..13699
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14124..14918
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      join(15005..15471,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
      361 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
      421 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgct
      481 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tttccgggag ctgcatgtgt
      541 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
      601 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
      661 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
      721 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
      781 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
      841 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
      901 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
      961 gtacgtgcta tccacaggaa agaggccttt tcgacctttt tcccctgcta gggcaatttg
     1021 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
     1081 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
     1141 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
     1201 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
     1261 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
     1321 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
     1381 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
     1441 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
     1501 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
     1561 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
     1621 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
     1681 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
     1741 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
     1801 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
     1861 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
     1921 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
     1981 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
     2041 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
     2101 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     2161 caggcaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     2221 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     2281 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     2341 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     2401 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     2461 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     2521 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     2581 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     2641 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     2701 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     2761 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     2821 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     2881 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     2941 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     3001 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     3061 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     3121 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     3181 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     3241 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     3301 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     3361 gcacggttga gccgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     3421 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
     3481 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
     3541 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
     3601 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
     3661 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
     3721 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
     3781 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
     3841 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
     3901 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
     3961 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
     4021 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
     4081 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
     4141 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
     4201 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
     4261 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
     4321 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
     4381 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     4441 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
     4501 cactgatagt ttgagctcct tatctttaat catattccat agtccatacc atagcacata
     4561 cagtagttat atgctgcaga agagatccaa caaaacattc acaatggatt atagaaacat
     4621 ttgtttattc attataatgt gatcttacat tcatttaata ttagaaaaag ccacaaattc
     4681 ataacacaac aagccaagaa aaaaacacaa acttaagcac acaagctttt tatttgacac
     4741 accaaatatt tcatcttcat cttcatattc tagatcactt ctttttctta gcctgtccgg
     4801 cctttttggt ggcagcagga cgcttacttg tttgggatgg ttcctgggct ggagtctgat
     4861 cctccctctc cgccggcggc gccttggact tgctagcctt cttcgttttt ctggccgggg
     4921 ccgtcccttg tgcatcccta ggttcctctc tcttcggcat tgttttgcct gtgagagcca
     4981 cctgagtgag attatgcgta gccacatcaa gatccgcatt acaggtcttc ccgcacgaaa
     5041 ggcattgaaa ggcctcaccg tcccggttcc cgacttcaca atgtccacac tttgggcatg
     5101 tgatggacgt tctttccggc ctcacctcga agacataaaa ggagcgatgc gtccggagat
     5161 cactaaaggc cttgtgcaat ccttgtatga accagcggtt ttctttttta gctgtaaaga
     5221 aattgtccca tccgggcaac cgctttccag aaccatgaaa gaagcgaaca ttgaggtctt
     5281 cgatgaccgg aataacaatt tgacactgag tcctccggcg tgtcttctct ataacgtaat
     5341 taatcgacct tctacagagt tcttcctttc tcctggagag tttaagatac tctggagatg
     5401 tccgcttcaa ggcccaaagt gcttcgttct tcaatttctg cgcttcaact gacagtctcg
     5461 gtttgtaagc gcgtgcccat gtgcggtctt tcctcataac ttccaccgga gctttctttt
     5521 tcgcgccttt tttcggggcg ggtgtgaaaa agacttggtc gcgactaacg ctgttactaa
     5581 gcagggcctc ggagatgaag gtagtattcg acgacatttt atcccaaggg agtctcttag
     5641 gatcaagccc aaaatcagca cacaattgag ttctggcagt ctctttagaa acgccatcca
     5701 gtgccctaac ctcagcctgc tgtgcttctg gcaaggcttc aacactgcga gcgcggagtt
     5761 cctcctcgtt ccggtcccag gcgattctgt aggcactgat gtctttaaga agatcatcgg
     5821 ggagtgtaaa gcgatcgaga ggctcgcact gaagtgcacc attctcctga gtaaccctgc
     5881 tgatcccggc actaattggg ttggtttgcc caaggtctat ggccaccaaa gcgactgtct
     5941 gcgtggctgt caacttatcc agtgtggcct tagtctgctt tcctttcagg gtccacttgc
     6001 gagcgtatgt tccgcatgcg tccagagtat aagtaaaggt gactatgttc cgcctcacat
     6061 caataactgg gtcgccagtg aacagatcga gaagcgcgtt cagactaatg tccttcggcg
     6121 caatagtcct ccaccgtgcg ttcctcaaaa gccctctaac gtcaatgacg acccaatccg
     6181 tgcctatcct cacggtgaca aggagtgcat cttgggcctt ctccttctct gaccgccaat
     6241 acctcctcat tctcttattt tttttaggac tcaatcccgg caccgtaacg gctttgccgg
     6301 ttttaggtga tatcgcagtc ccagcctcgc gctgccactc gggtatatac cctgggcagc
     6361 ctttctgtat atcgcatcta ttcctaacga cgcccaacgg tataggcgcg tttgggtccc
     6421 taacgtagcc agcgtactca ggcggaagga cgatctcatc gcgggggcga taagcctgag
     6481 ggctgatagt ctgataaaca taaaaggaag ggtttattcc gggtggctgc agcaagtgtc
     6541 ccgtctcatt cgttgcaact tcttcttctt ctgccttgat ttccgggagt ccggctgcag
     6601 cgcgagccgc gttgatggct gcaaggcgcg ctcttgcttt ttcattcctc agttgaacct
     6661 tcttcaacac cccatcgtag cgtcccagtg tatggtcaaa gatcaggtta agaccctgaa
     6721 catggctata tccatgatta gacacccctg tagcagcaaa ccatgccgcg tgcgactcag
     6781 aggattttcc aggcttacac gccgctcttt cagtagtgct aagggcgtag atatagcgct
     6841 gtatggcttc acttgccttc atgattggcc actccgcaaa atcccgggac ttcgtgacca
     6901 catggcactt tgctggaggt tgaaagttcg gtggttcctc ctcggatttg ccctggagat
     6961 atgccacgac ggcttcctct ccttgagccg ccagaatctt cccccctctt ttcatgtagg
     7021 atgatctgaa tctctcgcca gggaagtgct ttttcaacac tttagagaat tcgctttcga
     7081 cagccggttt aggagccgca ggcaccccgt gaataccaac cttccgcttc ttcttaggag
     7141 ccatcctgca ggctgcagaa gtaacaccaa acaacagggt gagcatcgac aaaagaaaca
     7201 gtaccaagca aataaatagc gtatgaaggc agggctaaaa aaatccacat atagctgctg
     7261 catatgccat catccaagta tatcaagatc aaaataatta taaaacatac ttgtttatta
     7321 taatagatag gtactcaagg ttagagcata tgaatagatg ctgcatatgc catcatgtat
     7381 atgcatcagt aaaacccaca tcaacatgta tacctatcct agatcgatat ttccatccat
     7441 cttaaactcg taactatgaa gatgtatgac acacacatac agttccaaaa ttaataaata
     7501 caccaggtag tttgaaacag tattctactc cgatcttgaa cgaatgaacg accgcccaac
     7561 cacaccacat catcacaacc aagcgaacaa aaagcatctc tgtatatgca tcagtaaaac
     7621 ccgcatcaac atgtatacct atcctagatc gatatttcca tccatcatct tcaattcgta
     7681 actatgaata tgtatggcac acacatacag atccaaaatt aataaatcca ccaggtagtt
     7741 tgaaacagaa ttaattctac tccgatcttg aacgaccgcc caaccagacc acatcatcac
     7801 aaccaagaca aaaaaaagca tgaaaagatg acccgacaaa caagtgcacg gcatatattg
     7861 aaataaagga aaagggcaaa ccaaacccta tgcaacgaaa caaaaaaaat catgaaatcg
     7921 atcccgtctg cggaacggct agagccatcc caggattccc caaagagaaa cactggcaag
     7981 ttagcaatca gaacgtgtct gacgtacagg tcgcatccgt gtacgaacgc tagtagcacg
     8041 gatctaacac aaacacggat ctaacacaaa catgaacaga agtagaacta ccgggcccta
     8101 accatggacc ggaacgccga tcttgagaag gtagagaggg ggggggggga ggacgagcgg
     8161 cgtaccttga agcggaggtg ccgacgggtg gatttggggg agttctggtt gtgtgtgtgt
     8221 gcgctccgaa caacacgagg ttggggaaag agggtgtgga gggggtgtct atttattacg
     8281 gcgggcgagg aagggaaagc gaaggagcgg tgggaaagga atcccccgta gctgccggtg
     8341 ccgtgagagg aggaggaggc cgcctgccgt gccggctcac gtctgccgct ccgccacgca
     8401 atttctggat gccgacagcg gagcaagtcc aacggtggag cggaactctc gagaggggtc
     8461 cagaggcagc gacagagatg ccgtgccgtc tgcttcgctt ggcccgacgc gacgctgctg
     8521 gttcgctggt tggtgtccgt tagactcgtc gacggcgttt aacaggctgg cattatctac
     8581 tcgaaacaag aaaaatgttt ccttagtttt tttaatttct taaagggtat ttgtttaatt
     8641 tttagtcact ttattttatt ctattttata tctaaattat taaataaaaa aactaaaata
     8701 gagttttagt tttcttaatt tagaggctaa aatagaataa aatagatgta ctaaaaaaat
     8761 tagtctataa aaaccattaa ccctaaaccc taaatggatg tactaataaa atggatgaag
     8821 tattatatag gtgaagctat ttgcaaaaaa aaaggagaac acatgcacac taaaaagata
     8881 aaactgtaga gtcctgttgt caaaatactc aattgtcctt tagaccatgt ctaactgttc
     8941 atttatatga ttctctaaaa cactgatatt attgtagtac tatagattat attattcgta
     9001 gagtaaagtt taaatatatg tataaagata gataaactgc acttcaaaca agtgtgacaa
     9061 aaaaaatatg tggtaatttt ttataactta gacatgcaat gctcacctta tctcaagaga
     9121 ggggcacgac cgggtcacgc tgcactgcag gcgcgcccac atcagtctct gcacaaagtg
     9181 catcctgggc tgcttcaatt ataaagcccc attcaccaca tttgctagat agtcgaaaag
     9241 caccatcaat attgagcttc aggtattttt ggttgtgttg tggttggatt gattctaata
     9301 tataccaaat caatataatt cactaccaaa atataccata gccatcacaa ctttattaat
     9361 tttggtagct taagatggta tatataataa ccaattaaca actgattcta attttactac
     9421 ggcccagtat ctaccaatac aaaacaacga gtatgttttc ttccgtcgta atcgtacaca
     9481 gtacaaaaaa acctggccag cctttcttgg gctggggctc tctttcgaaa ggtcacaaaa
     9541 cgtacacggc agtaacgccg cttcgctgcg tgttaacggc caccaacccc gccgtgagca
     9601 aacggcatca gctttccacc tcctcgatat ctccgcggcg ccgtctggac ccgccccctt
     9661 tccgttcctt tctttccttc tcgcgtttgc gtggtgggga cggactcccc aaaccgcctc
     9721 tccctctctt tatttgtcta tattctcact gggccccacc caccgcaccc ctgggcccac
     9781 tcacgagtcc ccccctcccc acctataaat accccacccc ctcctcgcct cttcctccat
     9841 caatcgaatc cccaaaatcg cagagaaaaa aaaatctccc ctcgaagcga agcgtcgaat
     9901 cgccttctca aggtatgcga ttttctgatc ctctccgttc ctcgcgtttg atttgatttc
     9961 ccggcctgtt cgtgattgtg agatgttgtt gttagtctcc gttttgcgat ctgtggtaga
    10021 tttgaacagg gttagatggg gttcgcgtgg tatgctggat ctgtgattat gagcgatgct
    10081 gttcgtggtc caagtattta ttggttcgga tctagaagta gaactgtgct agggttgtga
    10141 tttgttccga tctgttcaat cagtaggatt tagtctctgt ttttctcgtt gatccaagta
    10201 gcagcttcag gtatattttg cttaggttgt ttttgattca gtccctctag ttgcatagat
    10261 tctactctgt tcatgtttaa tctaagggct gcgtcttgtt gattagtgat tacatagcat
    10321 agctttcagg atattttact tgcttatgcc tatcttatca actgttgcac ctgtaaattc
    10381 tagcctatgt taattaacct gccttatgtg ctctcgggat agtgctagta gttattgaat
    10441 cagtttgccg atggaattct agtagttcat agacctgcag attatttttg tgaactcgag
    10501 cacggtgcgt ctctctattt tgttaggtca ctgttggtgt tgataggtac actgatgtta
    10561 ttgtggttta gatcgtgtat ctaacatatt ggaataattt gattgactga tttctgctgt
    10621 acttgcttgg tattgttata atttcatgtt catagttgct gaccatgctt cggtaattgt
    10681 gtgtgcagat ccagatcgga tcctcgttgc tgatgagtcc gtgaggacga aacgagtaag
    10741 ctcgtccaac gattgcccct cacgagggga cggagaccgg gcgtgtcaat aatatcactc
    10801 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc
    10861 atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac
    10921 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc
    10981 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac
    11041 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg
    11101 acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat
    11161 acgcatactg ttatctggct tggtctcagg ccggcatggt cccagcctcc tcgctggcgc
    11221 cggctgggca acatgcttcg gcatggcgaa tgggacggta cccggccgga attcgaccca
    11281 gctttcttgt acaaagtggt tcgataattc cttaattcga atttccccga tcgttcaaac
    11341 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
    11401 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
    11461 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
    11521 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactacta
    11581 gtcccatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
    11641 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
    11701 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
    11761 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg
    11821 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
    11881 cgtcttcaaa gcaagtggat tgatgtgaac atggtggagc acgacactct cgtctactcc
    11941 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
    12001 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
    12061 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
    12121 gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
    12181 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
    12241 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
    12301 ggaagttcat ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta
    12361 tctctcctag gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
    12421 aaaagttcga cagcgtgtcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
    12481 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
    12541 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
    12601 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
    12661 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
    12721 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
    12781 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
    12841 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
    12901 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
    12961 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
    13021 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
    13081 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
    13141 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
    13201 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
    13261 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
    13321 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
    13381 cgggatcttg gaggtgatta attaacgatc gacaagctct attttctcca taataatgtg
    13441 tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca
    13501 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
    13561 attcctaaaa ccaaaatcca gtactaaaat ccagatcccc cgaattaatt cggcgttaat
    13621 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
    13681 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
    13741 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
    13801 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
    13861 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
    13921 gttgctgcct gtgatcaccg cggtttcaaa atcggctccg tcgatactat gttatacgcc
    13981 aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg
    14041 aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt
    14101 agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac
    14161 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
    14221 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
    14281 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
    14341 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
    14401 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
    14461 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
    14521 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
    14581 atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg
    14641 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
    14701 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
    14761 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg
    14821 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
    14881 aaataaaata ttatatttta ctggatgaat tgttttagta cctagaatgc atgaccaaaa
    14941 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    15001 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    15061 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    15121 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
    15181 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    15241 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    15301 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    15361 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    15421 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga g
//
