LOCUS       sequence-429161-        8931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon plasmid for making Cre and Flpo reporter cell 
            lines using piggyBac transposase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lea NE, Lavin TK, Fu X, Matsuyama M, Hou 
            Y, Feng G, Wickersham IR
  TITLE     Third-generation rabies viral vectors allow nontoxic retrograde 
            targeting of projection neurons with greatly increased efficiency.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100644. doi: 
            10.1016/j.crmeth.2023.100644.
  PUBMED    37989085
REFERENCE   2  (bases 1 to 8931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100644. doi"; pages: "
            10.1016/j.crmeth.2023.100644"
COMMENT     Sequence Label: pB-CAG-FLEX-F14F15-BFP-(mCherry)'
FEATURES             Location/Qualifiers
     source          1..8931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1419..1722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1724..1999
                     /label=chicken beta-actin promoter
     intron          2000..3016
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2940..2962
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3024..3043
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(3071..3104)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3155..3188)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             3345..4046
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             complement(4049..4759)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4327..4346)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4596..4614
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      4756..4765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4915..4948
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4999..5032
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    5038..5626
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5091..5111)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5509..5520)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5630..5854
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6385..6419
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6910..6926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6910..6926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6923..6945)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6934..6950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6958..6964,6965..6982,6983..6988))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7003..7024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7141..7158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7312..7900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7392..7411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8071..8862,8863..8931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8694..8713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gttattaata gtaatcaatt
     1381 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     1441 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     1501 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     1561 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     1621 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     1681 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
     1741 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     1801 ttttaattat tttgtgcagc grkgggggsg gggggggggg gggsgcgcgc caggcggggc
     1861 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
     1921 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     1981 gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc
     2041 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     2101 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     2161 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     2221 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     2281 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     2341 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     2401 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     2461 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     2521 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     2581 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg
     2641 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     2701 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     2761 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     2821 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     2881 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     2941 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     3001 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     3061 caaagaattc ataacttcgt atagcataca ttatacgaag ttattctttg caccattcta
     3121 aagaataaca gtgataattt ctgggttaag gcaaataact tcgtatagga tactttatac
     3181 gaagttatgc taggatcatt ttggcaaaga attcgaagtt cctattctat cagaagtata
     3241 ggaacttcgc agaatggtag ctggattgta gctgctatta gcaatatgaa acctcttaga
     3301 agttcctatt cttataggag tataggaact tcgctagcgc caccatgagc gagctgatta
     3361 aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt
     3421 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
     3481 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca
     3541 gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg
     3601 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
     3661 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     3721 tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga
     3781 cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg
     3841 gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa cccgctaaga
     3901 acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca
     3961 acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta
     4021 gcaaactggg gcacaagctt aattaaggtt acttgtacag ctcgtccatg ccgccggtag
     4081 agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg
     4141 tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct
     4201 tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct
     4261 tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc
     4321 ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca
     4381 ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc
     4441 cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt
     4501 agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg
     4561 acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct
     4621 gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca
     4681 cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct
     4741 cctcgccctt gctcaccatg gtggcggcgc gccgaagttc ctatacttct gatagaatag
     4801 gaacttcttg ccttaaccca gaaattatca ctgttattct ttagaatggt gcaaagagaa
     4861 gttcctatac tcctataaga ataggaactt ctccggaatc aacctctgac gcgtataact
     4921 tcgtataatg tatgctatac gaagttatta agaggtttca tattgctaat agcagctaca
     4981 atccagctac cattctgcat aacttcgtat aaagtatcct atacgaagtt attccggaat
     5041 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5101 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5161 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5221 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5281 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5341 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5401 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     5461 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5521 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5581 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgc tgtgccttct
     5641 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5701 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5761 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5821 agcaggcatg ctggggatgc ggtgggctct atggagatcc gcggcccatt ggtatggctt
     5881 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
     5941 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
     6001 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
     6061 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct gatatctata
     6121 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
     6181 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
     6241 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
     6301 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     6361 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt
     6421 taatctagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt catcgcccaa
     6481 gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag
     6541 cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca
     6601 cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg
     6661 ttcaggccac ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca
     6721 gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg
     6781 tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg
     6841 gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcttggc
     6901 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6961 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7021 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7081 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7141 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7201 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7261 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7321 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7381 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7441 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7501 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7561 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7621 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7681 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7741 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7801 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7861 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7921 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7981 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8041 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8101 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8161 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8221 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8281 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8341 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8401 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8461 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8521 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8581 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8641 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8701 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8761 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8821 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8881 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca t
//
