LOCUS       sequence-429164-       10670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a
            cassette for gRNA constitutive expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Prezza G, Liao C, Reichardt S, Beisel CL, Westermann AJ
  TITLE     CRISPR-based screening of small RNA modulators of bile 
            susceptibility in Bacteroides thetaiotaomicron.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Feb 6;121(6):e2311323121. doi: 
            10.1073/pnas.2311323121. Epub 2024 Jan 31.
  PUBMED    38294941
REFERENCE   2  (bases 1 to 10670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-02-6"; volume: "121(6):e2311323121. 
            doi"; pages: " 10.1073/pnas.2311323121. Epub 2024 Jan 31"
COMMENT     Sequence Label: AWP-029
FEATURES             Location/Qualifiers
     source          1..10670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             213..1295
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(232..251)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(4452..4520,4521..5312)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTSLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4670..4689)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            5443..5552
                     /note="incP origin of transfer"
     rep_origin      5723..6111
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    6185..6201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    6218..6234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             6293..6313
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10085..10150
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 actcaggaat aaattataaa ttaaggtaag aagattgtag gataagctaa tgaaatagaa
       61 aaaggatgcc gtcacacaac ttgtcggcat tcttttttgt tttattagtt gaaaatatag
      121 tgaaaaagtt gcctaaatat gtatgttaac aaattatttg tcgtaacttt gcactccaaa
      181 tctgttttta acatatggca ctagtggtga atgtgaaacc agtaacgtta tacgatgtcg
      241 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
      301 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
      361 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
      421 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
      481 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
      541 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
      601 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
      661 tctctgacca gacacccatc aacagtatta ttttctccca tgaggacggt acgcgactgg
      721 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
      781 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
      841 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
      901 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
      961 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
     1021 tagtgggata cgacgatacc gaggacagct catgttatat cccgccgtta accaccatca
     1081 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     1141 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     1201 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1261 cacgacaggt ttcccgactg gaaagcgggc agtgagcttt cctcggtacc aaattccaga
     1321 aaagaggcct cccgaaaggg gggccttttt tcgttttggt cctacttgtg cctgttctat
     1381 ttccgaaccg accgcttgta tgaatgcatc aaaattcgtt ttctctacgt tggattcctt
     1441 gttgctcata ttgtgatgat aatttctaca aatatagtca ttggtaacta tctatgaaac
     1501 tgtttgatac ttttatagtt gattaaactt gttcatggca tttgccttaa tatcatccgc
     1561 tatgtcaatg tagggtttca tagctttgta gtcgctgtgt cccgtccatt tcatgaccac
     1621 ctgtgccggg attccgagag ccagcgcatt gcagatgaat gtcctttttc ctgcatgggt
     1681 actgagcaaa gcgtatttgg gtgtgacttc atcaatacgt tcatttccct tgtagtaggt
     1741 ttcccgtaca ggctcgttga tttctgccag ttcgcccagc tctttcaggt aatcgttcat
     1801 cttctggttg ctgatgacgg gcagagccat gtaattctcg aaatggatgt ccttgtattt
     1861 gtccagtatg gctttgctgt atttgttcag ttcaatcgtc aggctgtcgg cagtcttgac
     1921 tgtggttatt tcgatgtggt cggacttcac atcgcttctt ttcagattgc gaacatccga
     1981 ataccgcaaa ctcgtaaagc agcagaacag gaaaacatca cgcacacgtt ccaggtattg
     2041 cttatccttg ggtatctggt agtctttcag cttgttcagt tcatcccaag tcaggaagat
     2101 tacttttttc gaggtggttt tcagtttcgg tttgaacgta tcgtatgcaa tgttctgatg
     2161 atgtcctttc ttgaagctcc agcgcaggaa ccatttgagg aatcccattt gcttgccgat
     2221 ggtgctgttt ctcatatcct tggtgtcacg caggaagttg acgtattcgt tcaatccaaa
     2281 ctcgttgaaa tagttgaacg ttgcatcctc cttgaactct ttgaggtggt tcctcactgc
     2341 tgcaaatttt tcataggtgg atgccgtcca gttattctgg ttaccgcact cttttacaaa
     2401 ctcatcgaac acctcccaaa agctgacagg ggcttcttcc ggctgttctt cactggtgtc
     2461 tttcattctc atgttgaaag cttccttcaa ctgttgggtc gttggcatga cctcctgcac
     2521 ctcaaattcc ttgaaaatat tctggatttc ggcatagtat ttcagcaagt ccgtattgat
     2581 ttcggctgca ctttgcttta gcttgttggt acatccgttc tttacccgct gcttatctac
     2641 atcccatttg gctacgtcaa tccggtagcc cgttgtaaac tcgatacgtt ggctggcaaa
     2701 gatgacacgc atacggatgg gtacgttctc tacgattggc acaccgttct ttttccggct
     2761 ctccaatgca aaaatgatgt tgcgcttgat attcataatt gggtgcgttt gaaattctac
     2821 acccaaatat acacccaatt attgagatag caaaagacat ttagaaacat ttacttttac
     2881 tctatattgt aatttacact tgattatcag tcgtttgcag ttttatgata ttctgtgaaa
     2941 gtataagttc gagagcctgt ctctccgcaa aaaacgctga aaatcagcag attgcaaaac
     3001 aaacaccctg ttttacaccc aagaatgtaa agtcgggtgt ttttgtttta tttaagataa
     3061 tacaaccact acataataaa agagtagcga tattaaaaga atccgatgag aaaagacaaa
     3121 tatttatcta tccattcagt ttgatttctc aggactttac atcgtcctga aagtatttgt
     3181 tggtaccgag gacgcgtaaa catttacagt tgcatgtggc ctattgtttt tagccgttaa
     3241 atattttata actattaaat agcgatacaa attgttcgaa actaatattg tttatatcat
     3301 atattctcgc atgttttaaa gctttattaa attgattttt tgtaaacagt ttttcgtact
     3361 ctttgttaac ccatttcatt acaaaagttt catatttttt tctctcttta aatgccattt
     3421 ttgctggctt tctttttaat acaattaatg tgctatccac tttaggtttt ggatggaaat
     3481 aatacctagg aatttttgct aatatagaaa tatctacctc tgccattaac agcaatgcta
     3541 gtgatctgtt tgtatctaat aacattttag caaaaccata ttccactatt aaataactta
     3601 ttgtggctga actttcaaaa acaatttttc gaattatatt tgtgcttatg ttgtaaggta
     3661 tgctgccaaa tattttatat ggattgtggc taggaaatgt aaatttcagt atatcatcat
     3721 ttactatttg atagttagga taatttaaga gcttattacg agttacctca cataatttag
     3781 aatcaatttc tatcgccgtt acaaaattac atctctttac caatccagca gtaaaatgac
     3841 ctttccctgc acctatttca aagatgttat ctttttcatc taaacttatg caattcatta
     3901 ttttttctat gtgatatttt gaagtaataa aattttgact atcttttata tttactttgt
     3961 tcattataac ctctccttaa tttattgcat ctcttttcga atatttatgt tttttgagaa
     4021 aagaacgtac tcatggttca tcccgatatg cgtatcggtc tgtatatcag caactttcta
     4081 tgtgtttcaa ctacaatagt catctattct catctttctg agtccacccc ctgcaaagcc
     4141 cctctttacg acataaaaat tcggtcggaa aaggtatgca aaagatgttt ctctctttaa
     4201 gagaaactct tcgggatgca aaaatatgaa aataactcca attcaccaaa ttatatagcg
     4261 acttttttac aaaatgctaa aatttgttga tttccgtcaa gcaattgttg agcaaaaatg
     4321 tcttttacga taaaatgata cctcaatatc aactgtttag caaaacgata tttctcttaa
     4381 agagagaaac acctttttgt tcaccaatcc ccgactttta atcccgcggc catgattgaa
     4441 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4501 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4561 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4621 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4681 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4741 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4801 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4861 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4921 taactagcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4981 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5041 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5101 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5161 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5221 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5281 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcataacgcg
     5341 tcaattcgag ggggatcaat tccgtgatag gtgggctgcc cttcctggtt ggcttggttt
     5401 catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag
     5461 caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc
     5521 tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa
     5581 agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg
     5641 aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaacgga attgatccgg
     5701 ccacgatgcg tccggcgtag aggatctgaa gatcagcagt tcaacctgtt gatagtacgt
     5761 actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt
     5821 taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct
     5881 catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa tcagcaactt
     5941 aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag gcttttaaag
     6001 cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg agaagccctt
     6061 agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac atgggaattc
     6121 ccctccaccg cggtggtaca aagaaaattc gacaaactgt tatttttcta tctatttatt
     6181 tgaattgtga gcggataaca attacctttg tcggcaattg tgagcggata acaattaaat
     6241 aaagatattc tcgtcaaaca aatataaata atataaacgg cgcgccatgg ccccaaagaa
     6301 gaaacggaag gttatgcaaa taaacaacct caagatcata tatatgaagt tcacagattt
     6361 caccggcctg tatagcttgt ccaagactct caggttcgag ttgaagccga tcgggaagac
     6421 actcgaaaac ataaaaaagg cgggactttt ggagcaagat cagcataggg cggactccta
     6481 caagaaggtg aagaaaatca ttgatgaata tcacaaggct tttatagaaa agtcgctttc
     6541 taatttcgaa ctcaaatacc aatcggaaga caagctcgac tcgttggaag aatatctgat
     6601 gtactactcg atgaagagaa ttgaaaagac cgaaaaagat aagttcgcca aaatccagga
     6661 caacctcaga aaacagatcg cggaccacct taaaggcgat gagtcttaca aaacaatatt
     6721 ttccaaggat ttgatcagga agaatttgcc ggacttcgtc aagagcgacg aggaacggac
     6781 tttgattaag gagtttaagg acttcaccac gtactttaaa gggttctacg aaaatagaga
     6841 gaatatgtac agcgcggagg ataaaagcac cgccatctcc catagaatca tccacgagaa
     6901 tctccctaag tttgtggata acataaacgc gttctccaaa ataatcctca tcccagagct
     6961 gcgggagaag ctcaatcaga tataccagga cttcgaagag tacctgaacg tggagtcaat
     7021 tgatgaaata ttccatcttg actacttcag catggttatg acacaaaagc agatcgaagt
     7081 ctataacgcc attattggcg ggaagtctac caacgacaag aagatacagg ggcttaatga
     7141 gtatattaat ctgtataacc aaaagcacaa agattgcaag ttgccaaagc tgaaactgtt
     7201 gttcaagcag attcttagcg accggatagc gatctcttgg ctcccggata acttcaagga
     7261 tgatcaggag gcgcttgact caatagatac atgctacaag aatctcctga atgacgggaa
     7321 cgtgcttggg gaaggaaatc tgaagctgct gctcgagaac atagatacat acaatctgaa
     7381 gggaattttt attagaaatg atctccaact gacagatatt tcacagaaga tgtacgcttc
     7441 ttggaatgtc attcaagatg cagttattct tgatttgaaa aagcaggttt cccgcaagaa
     7501 gaaggagagc gcggaagatt acaacgatag actgaagaag ctctatacga gccaggagag
     7561 cttttcgata cagtacctca acgactgctt gagggcgtac gggaaaacag aaaacattca
     7621 agattacttt gcgaaactgg gggcggtcaa caacgagcac gagcagacga tcaacttgtt
     7681 tgcacaagtt aggaacgcct atacttccgt tcaagccatc ctcacaacac cgtaccctga
     7741 gaatgccaac ttggctcagg acaaagagac cgtcgcactt atcaagaacc tccttgattc
     7801 actgaagcgc cttcaaaggt ttatcaaacc tctcctcggc aagggcgatg agagcgacaa
     7861 ggacgagcgc ttctacggag acttcactcc cctttgggag acgctcaacc aaataacccc
     7921 attgtataat atggtgagaa actacatgac aaggaaacct tattcgcagg agaaaataaa
     7981 gctcaatttt gaaaactcta ccttgcttgg gggatgggat ctgaacaaag agcacgataa
     8041 tactgcaatc atacttcgca agaatggtct ttactacctg gctatcatga aaaaaagcgc
     8101 caacaagatc ttcgataagg acaaattgga caactctggc gactgttacg aaaaaatggt
     8161 gtataaactg ctcccaggcg ccaacaaaat gcttcccaag gttttctttt ccaagtcgcg
     8221 catcgacgag ttcaaacctt ctgagaacat aatcgagaac tacaagaagg gaactcacaa
     8281 aaaaggggcc aacttcaatc tcgcagattg ccacaacttg atcgatttct ttaaatcctc
     8341 tatatcgaag catgaagact ggtccaagtt caactttcat ttcagcgaca cctcatctta
     8401 tgaggacctt tcagacttct acagagaggt ggagcaacaa gggtactcaa tctcattttg
     8461 cgatgtcagc gtggagtaca ttaacaagat ggttgagaag ggggaccttt acctcttcca
     8521 aatctacaat aaggatttct cggaattcag caagggcacg cctaatatgc acacgctcta
     8581 ttggaacagc ctcttctcga aagagaacct gaataatata atatacaaac tcaatggcca
     8641 agctgagatt ttcttcagga agaagtcact caattacaag cggccgacac acccggcgca
     8701 tcaagctatt aagaacaaga acaagtgtaa tgagaaaaaa gaatccatct tcgattatga
     8761 cctcgtcaag gataagcgct acaccgttga taaatttcag tttcacgttc caattaccat
     8821 gaactttaag tctacaggaa ataccaatat aaaccaacag gtcattgact atcttcgcac
     8881 ggaggacgat acgcacatca tcggaatcgc tcggggggag cggcacctgc tttatttggt
     8941 ggttatcgac tcccatggga aaatcgtgga acagttcacc ctcaatgaga ttgtgaacga
     9001 gtacggcgga aacatttata gaacgaatta ccacgacttg ctggacacga gagagcaaaa
     9061 cagggagaaa gcgagggagt catggcagac aatcgagaac ataaaagaat tgaaagaggg
     9121 ctatatctct caagtgattc ataaaataac ggatctcatg caaaagtacc atgcagtcgt
     9181 cgtgctcgag gatctcaaca tgggattcat gaggggaagg cagaaggtgg agaagcaggt
     9241 ctatcaaaaa tttgaagaaa tgctgatcaa taaacttaat tatctggtca acaaaaaggc
     9301 tgatcagaat agcgctggag ggctgttgca cgcctaccag ctgacatcaa agttcgagtc
     9361 cttccagaaa cttggcaagc agtctggttt tcttttttac attccagcct ggaatacctc
     9421 caaaattgat cctgtcaccg gatttgtgaa cttgtttgac accaggtacg agagcataga
     9481 caaggcgaag gcattctttg gaaagttcga ttccataagg tataatgcgg acaaagactg
     9541 gttcgagttt gcgttcgact ataacaactt cacaactaaa gcagagggga ctagaacaaa
     9601 ttggacgatc tgtacatacg gttcaaggat caggaccttc aggaatcaag cgaagaattc
     9661 tcaatgggac aacgaggaga tcgatctgac aaaagcctac aaggcttttt tcgccaaaca
     9721 tggaatcaat atctatgata atatcaagga ggccatagcc atggaaactg agaaaagctt
     9781 ctttgaggac ctgcttcatc tgcttaaatt gactctgcag atgcggaact ccattaccgg
     9841 cacaaccact gattatctca tttcgccagt ccatgactct aaaggaaact tctacgatag
     9901 caggatctgt gataatagcc ttcctgccaa cgcggacgcg aacggcgcgt ataatattgc
     9961 ccgcaaagga ttgatgctca tacagcaaat caaggattcg acgagctcga accgctttaa
    10021 gttctcacct attacgaata aagattggct catttttgca caagagaagc cgtacttgaa
    10081 tgacgactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
    10141 cgatgacaag taagcggccg cattaatgcg gctgcaattt ttttgggcgg ggccgcccaa
    10201 aaaaatccta gcaccctgca gcagtactgc ttgaccataa gaacaaaaaa acttccctta
    10261 aggataaagt ttggaagata aagctaaaag ttcttatctt tgcagtaatt tctactgttg
    10321 tagatcccgg gccggcttat cggtcagttt cacctgattt acgtaaaaac ccgcttcggc
    10381 gggtttttgc ttttggaggg gcagaaagat gaatgactgt ccacgacgct atacccaaaa
    10441 gaaatctaga tgatgattaa ggatcttgcg tacaatttga cgatcgtttc acgctaagat
    10501 gctggctaat tgcctatctt ccagtgatgg aacagcattt gtgcattggc tgcaacaata
    10561 gccttgatct ggaagaagca atgaaagctg ctgttaagtc tccgaatcag gtattgttcc
    10621 tgacaggtgt attcccatcc ggtaaacgcg gatactttgc agttgatctg
//
