LOCUS       sequence-429166-       11794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a
            cassette for constitutive CRISPR array expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11794)
  AUTHORS   Prezza G, Liao C, Reichardt S, Beisel CL, Westermann AJ
  TITLE     CRISPR-based screening of small RNA modulators of bile 
            susceptibility in Bacteroides thetaiotaomicron.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Feb 6;121(6):e2311323121. doi: 
            10.1073/pnas.2311323121. Epub 2024 Jan 31.
  PUBMED    38294941
REFERENCE   2  (bases 1 to 11794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-02-6"; volume: "121(6):e2311323121. 
            doi"; pages: " 10.1073/pnas.2311323121. Epub 2024 Jan 31"
COMMENT     Sequence Label: AWP-031
FEATURES             Location/Qualifiers
     source          1..11794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      16..87
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      103..130
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             775..1857
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(794..813)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(5014..5082,5083..5874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTSLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5232..5251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            6005..6114
                     /note="incP origin of transfer"
     rep_origin      6285..6673
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    6747..6763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    6780..6796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             6855..6875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10647..10712
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     RBS             11067..11078
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(11085..11794,1..4)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 caaatgacca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
       61 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      121 cctttctgcg tttatacgtc tctaacggtc taagaacttt aaataatttg tctgtatatt
      181 attaagcttg gctgttttgg cggatgagag aagattttca gccccggctt atcggtcagt
      241 ttcacctgat ttacgtaaaa acccgcttcg gcgggttttt gcttttggag gggcagaaag
      301 atgaatgact gtccacgacg ctatacccaa aagaaatcta gatgatgatt aaggatcttg
      361 cgtacaattt gacgatcgtt tcacgctaag atgctggcta attgcctatc ttccagtgat
      421 ggaacagcat ttgtgcattg gctgcaacaa tagccttgat ctggaagaag caatgaaagc
      481 tgctgttaag tctccgaatc aggtattgtt cctgacaggt gtattcccat ccggtaaacg
      541 cggatacttt gcagttgatc tgactcagga ataaattata aattaaggta agaagattgt
      601 aggataagct aatgaaatag aaaaaggatg ccgtcacaca acttgtcggc attctttttt
      661 gttttattag ttgaaaatat agtgaaaaag ttgcctaaat atgtatgtta acaaattatt
      721 tgtcgtaact ttgcactcca aatctgtttt taacatatgg cactagtggt gaatgtgaaa
      781 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
      841 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
      901 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
      961 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
     1021 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
     1081 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
     1141 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
     1201 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
     1261 catgaggacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
     1321 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
     1381 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
     1441 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
     1501 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
     1561 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaggacag ctcatgttat
     1621 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
     1681 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtatca
     1741 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     1801 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagct
     1861 ttcctcggta ccaaattcca gaaaagaggc ctcccgaaag gggggccttt tttcgttttg
     1921 gtcctacttg tgcctgttct atttccgaac cgaccgcttg tatgaatgca tcaaaattcg
     1981 ttttctctac gttggattcc ttgttgctca tattgtgatg ataatttcta caaatatagt
     2041 cattggtaac tatctatgaa actgtttgat acttttatag ttgattaaac ttgttcatgg
     2101 catttgcctt aatatcatcc gctatgtcaa tgtagggttt catagctttg tagtcgctgt
     2161 gtcccgtcca tttcatgacc acctgtgccg ggattccgag agccagcgca ttgcagatga
     2221 atgtcctttt tcctgcatgg gtactgagca aagcgtattt gggtgtgact tcatcaatac
     2281 gttcatttcc cttgtagtag gtttcccgta caggctcgtt gatttctgcc agttcgccca
     2341 gctctttcag gtaatcgttc atcttctggt tgctgatgac gggcagagcc atgtaattct
     2401 cgaaatggat gtccttgtat ttgtccagta tggctttgct gtatttgttc agttcaatcg
     2461 tcaggctgtc ggcagtcttg actgtggtta tttcgatgtg gtcggacttc acatcgcttc
     2521 ttttcagatt gcgaacatcc gaataccgca aactcgtaaa gcagcagaac aggaaaacat
     2581 cacgcacacg ttccaggtat tgcttatcct tgggtatctg gtagtctttc agcttgttca
     2641 gttcatccca agtcaggaag attacttttt tcgaggtggt tttcagtttc ggtttgaacg
     2701 tatcgtatgc aatgttctga tgatgtcctt tcttgaagct ccagcgcagg aaccatttga
     2761 ggaatcccat ttgcttgccg atggtgctgt ttctcatatc cttggtgtca cgcaggaagt
     2821 tgacgtattc gttcaatcca aactcgttga aatagttgaa cgttgcatcc tccttgaact
     2881 ctttgaggtg gttcctcact gctgcaaatt tttcataggt ggatgccgtc cagttattct
     2941 ggttaccgca ctcttttaca aactcatcga acacctccca aaagctgaca ggggcttctt
     3001 ccggctgttc ttcactggtg tctttcattc tcatgttgaa agcttccttc aactgttggg
     3061 tcgttggcat gacctcctgc acctcaaatt ccttgaaaat attctggatt tcggcatagt
     3121 atttcagcaa gtccgtattg atttcggctg cactttgctt tagcttgttg gtacatccgt
     3181 tctttacccg ctgcttatct acatcccatt tggctacgtc aatccggtag cccgttgtaa
     3241 actcgatacg ttggctggca aagatgacac gcatacggat gggtacgttc tctacgattg
     3301 gcacaccgtt ctttttccgg ctctccaatg caaaaatgat gttgcgcttg atattcataa
     3361 ttgggtgcgt ttgaaattct acacccaaat atacacccaa ttattgagat agcaaaagac
     3421 atttagaaac atttactttt actctatatt gtaatttaca cttgattatc agtcgtttgc
     3481 agttttatga tattctgtga aagtataagt tcgagagcct gtctctccgc aaaaaacgct
     3541 gaaaatcagc agattgcaaa acaaacaccc tgttttacac ccaagaatgt aaagtcgggt
     3601 gtttttgttt tatttaagat aatacaacca ctacataata aaagagtagc gatattaaaa
     3661 gaatccgatg agaaaagaca aatatttatc tatccattca gtttgatttc tcaggacttt
     3721 acatcgtcct gaaagtattt gttggtaccg aggacgcgta aacatttaca gttgcatgtg
     3781 gcctattgtt tttagccgtt aaatatttta taactattaa atagcgatac aaattgttcg
     3841 aaactaatat tgtttatatc atatattctc gcatgtttta aagctttatt aaattgattt
     3901 tttgtaaaca gtttttcgta ctctttgtta acccatttca ttacaaaagt ttcatatttt
     3961 tttctctctt taaatgccat ttttgctggc tttcttttta atacaattaa tgtgctatcc
     4021 actttaggtt ttggatggaa ataataccta ggaatttttg ctaatataga aatatctacc
     4081 tctgccatta acagcaatgc tagtgatctg tttgtatcta ataacatttt agcaaaacca
     4141 tattccacta ttaaataact tattgtggct gaactttcaa aaacaatttt tcgaattata
     4201 tttgtgctta tgttgtaagg tatgctgcca aatattttat atggattgtg gctaggaaat
     4261 gtaaatttca gtatatcatc atttactatt tgatagttag gataatttaa gagcttatta
     4321 cgagttacct cacataattt agaatcaatt tctatcgccg ttacaaaatt acatctcttt
     4381 accaatccag cagtaaaatg acctttccct gcacctattt caaagatgtt atctttttca
     4441 tctaaactta tgcaattcat tattttttct atgtgatatt ttgaagtaat aaaattttga
     4501 ctatctttta tatttacttt gttcattata acctctcctt aatttattgc atctcttttc
     4561 gaatatttat gttttttgag aaaagaacgt actcatggtt catcccgata tgcgtatcgg
     4621 tctgtatatc agcaactttc tatgtgtttc aactacaata gtcatctatt ctcatctttc
     4681 tgagtccacc ccctgcaaag cccctcttta cgacataaaa attcggtcgg aaaaggtatg
     4741 caaaagatgt ttctctcttt aagagaaact cttcgggatg caaaaatatg aaaataactc
     4801 caattcacca aattatatag cgactttttt acaaaatgct aaaatttgtt gatttccgtc
     4861 aagcaattgt tgagcaaaaa tgtcttttac gataaaatga tacctcaata tcaactgttt
     4921 agcaaaacga tatttctctt aaagagagaa acaccttttt gttcaccaat ccccgacttt
     4981 taatcccgcg gccatgattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5221 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5461 ttgcacaaca tgggggatca tgtaactagc cttgatcgtt gggaaccgga gctgaatgaa
     5521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5881 gaccaagttt actcataacg cgtcaattcg agggggatca attccgtgat aggtgggctg
     5941 cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg
     6001 gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     6061 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
     6121 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     6181 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
     6241 gcggaaaacg gaattgatcc ggccacgatg cgtccggcgt agaggatctg aagatcagca
     6301 gttcaacctg ttgatagtac gtactaagct ctcatgtttc acgtactaag ctctcatgtt
     6361 taacgtacta agctctcatg tttaacgaac taaaccctca tggctaacgt actaagctct
     6421 catggctaac gtactaagct ctcatgtttc acgtactaag ctctcatgtt tgaacaataa
     6481 aattaatata aatcagcaac ttaaatagcc tctaaggttt taagttttat aagaaaaaaa
     6541 agaatatata aggcttttaa agcttttaag gtttaacggt tgtggacaac aagccaggga
     6601 tgtaacgcac tgagaagccc ttagagcctc tcaaagcaat tttgagtgac acaggaacac
     6661 ttaacggctg acatgggaat tcccctccac cgcggtggta caaagaaaat tcgacaaact
     6721 gttatttttc tatctattta tttgaattgt gagcggataa caattacctt tgtcggcaat
     6781 tgtgagcgga taacaattaa ataaagatat tctcgtcaaa caaatataaa taatataaac
     6841 ggcgcgccat ggccccaaag aagaaacgga aggttatgca aataaacaac ctcaagatca
     6901 tatatatgaa gttcacagat ttcaccggcc tgtatagctt gtccaagact ctcaggttcg
     6961 agttgaagcc gatcgggaag acactcgaaa acataaaaaa ggcgggactt ttggagcaag
     7021 atcagcatag ggcggactcc tacaagaagg tgaagaaaat cattgatgaa tatcacaagg
     7081 cttttataga aaagtcgctt tctaatttcg aactcaaata ccaatcggaa gacaagctcg
     7141 actcgttgga agaatatctg atgtactact cgatgaagag aattgaaaag accgaaaaag
     7201 ataagttcgc caaaatccag gacaacctca gaaaacagat cgcggaccac cttaaaggcg
     7261 atgagtctta caaaacaata ttttccaagg atttgatcag gaagaatttg ccggacttcg
     7321 tcaagagcga cgaggaacgg actttgatta aggagtttaa ggacttcacc acgtacttta
     7381 aagggttcta cgaaaataga gagaatatgt acagcgcgga ggataaaagc accgccatct
     7441 cccatagaat catccacgag aatctcccta agtttgtgga taacataaac gcgttctcca
     7501 aaataatcct catcccagag ctgcgggaga agctcaatca gatataccag gacttcgaag
     7561 agtacctgaa cgtggagtca attgatgaaa tattccatct tgactacttc agcatggtta
     7621 tgacacaaaa gcagatcgaa gtctataacg ccattattgg cgggaagtct accaacgaca
     7681 agaagataca ggggcttaat gagtatatta atctgtataa ccaaaagcac aaagattgca
     7741 agttgccaaa gctgaaactg ttgttcaagc agattcttag cgaccggata gcgatctctt
     7801 ggctcccgga taacttcaag gatgatcagg aggcgcttga ctcaatagat acatgctaca
     7861 agaatctcct gaatgacggg aacgtgcttg gggaaggaaa tctgaagctg ctgctcgaga
     7921 acatagatac atacaatctg aagggaattt ttattagaaa tgatctccaa ctgacagata
     7981 tttcacagaa gatgtacgct tcttggaatg tcattcaaga tgcagttatt cttgatttga
     8041 aaaagcaggt ttcccgcaag aagaaggaga gcgcggaaga ttacaacgat agactgaaga
     8101 agctctatac gagccaggag agcttttcga tacagtacct caacgactgc ttgagggcgt
     8161 acgggaaaac agaaaacatt caagattact ttgcgaaact gggggcggtc aacaacgagc
     8221 acgagcagac gatcaacttg tttgcacaag ttaggaacgc ctatacttcc gttcaagcca
     8281 tcctcacaac accgtaccct gagaatgcca acttggctca ggacaaagag accgtcgcac
     8341 ttatcaagaa cctccttgat tcactgaagc gccttcaaag gtttatcaaa cctctcctcg
     8401 gcaagggcga tgagagcgac aaggacgagc gcttctacgg agacttcact cccctttggg
     8461 aaacgctcaa ccaaataacc ccattgtata atatggtgag aaactacatg acaaggaaac
     8521 cttattcgca ggagaaaata aagctcaatt ttgaaaactc taccttgctt gggggatggg
     8581 atctgaacaa agagcacgat aatactgcaa tcatacttcg caagaatggt ctttactacc
     8641 tggctatcat gaaaaaaagc gccaacaaga tcttcgataa ggacaaattg gacaactctg
     8701 gcgactgtta cgaaaaaatg gtgtataaac tgctcccagg cgccaacaaa atgcttccca
     8761 aggttttctt ttccaagtcg cgcatcgacg agttcaaacc ttctgagaac ataatcgaga
     8821 actacaagaa gggaactcac aaaaaagggg ccaacttcaa tctcgcagat tgccacaact
     8881 tgatcgattt ctttaaatcc tctatatcga agcatgaaga ctggtccaag ttcaactttc
     8941 atttcagcga cacctcatct tatgaggacc tttcagactt ctacagagag gtggagcaac
     9001 aagggtactc aatctcattt tgcgatgtca gcgtggagta cattaacaag atggttgaga
     9061 agggggacct ttacctcttc caaatctaca ataaggattt ctcggaattc agcaagggca
     9121 cgcctaatat gcacacgctc tattggaaca gcctcttctc gaaagagaac ctgaataata
     9181 taatatacaa actcaatggc caagctgaga ttttcttcag gaagaagtca ctcaattaca
     9241 agcggccgac acacccggcg catcaagcta ttaagaacaa gaacaagtgt aatgagaaaa
     9301 aagaatccat cttcgattat gacctcgtca aggataagcg ctacaccgtt gataaatttc
     9361 agtttcacgt tccaattacc atgaacttta agtctacagg aaataccaat ataaaccaac
     9421 aggtcattga ctatcttcgc acggaggacg atacgcacat catcggaatc gctcgggggg
     9481 agcggcacct gctttatttg gtggttatcg actcccatgg gaaaatcgtg gaacagttca
     9541 ccctcaatga gattgtgaac gagtacggcg gaaacattta tagaacgaat taccacgact
     9601 tgctggacac gagagagcaa aacagggaga aagcgaggga gtcatggcag acaatcgaga
     9661 acataaaaga attgaaagag ggctatatct ctcaagtgat tcataaaata acggatctca
     9721 tgcaaaagta ccatgcagtc gtcgtgctcg aggatctcaa catgggattc atgaggggaa
     9781 ggcagaaggt ggagaagcag gtctatcaaa aatttgaaga aatgctgatc aataaactta
     9841 attatctggt caacaaaaag gctgatcaga atagcgctgg agggctgttg cacgcctacc
     9901 agctgacatc aaagttcgag tccttccaga aacttggcaa gcagtctggt tttctttttt
     9961 acattccagc ctggaatacc tccaaaattg atcctgtcac cggatttgtg aacttgtttg
    10021 acaccaggta cgagagcata gacaaggcga aggcattctt tggaaagttc gattccataa
    10081 ggtataatgc ggacaaagac tggttcgagt ttgcgttcga ctataacaac ttcacaacta
    10141 aagcagaggg gactagaaca aattggacga tctgtacata cggttcaagg atcaggacct
    10201 tcaggaatca agcgaagaat tctcaatggg acaacgagga gatcgatctg acaaaagcct
    10261 acaaggcttt tttcgccaaa catggaatca atatctatga taatatcaag gaggccatag
    10321 ccatggaaac tgagaaaagc ttctttgagg acctgcttca tctgcttaaa ttgactctgc
    10381 agatgcggaa ctccattacc ggcacaacca ctgattatct catttcgcca gtccatgact
    10441 ctaaaggaaa cttctacgat agcaggatct gtgataatag ccttcctgcc aacgcggacg
    10501 cgaacggcgc gtataatatt gcccgcaaag gattgatgct catacagcaa atcaaggatt
    10561 cgacgagctc gaaccgcttt aagttctcac ctattacgaa taaagattgg ctcatttttg
    10621 cacaagagaa gccgtacttg aatgacgact acaaagacca tgacggtgat tataaagatc
    10681 atgacatcga ttacaaggat gacgatgaca agtaagcggc cgcattaatg cggctgcaat
    10741 ttttttgggc ggggccgccc aaaaaaatcc tagcaccctg cagcagtact gcttgaccat
    10801 aagaacaaaa aaacttccct taaggataaa gtttggaaga taaagctaaa agttcttatc
    10861 tttgcagtgc tagcgaattc gagctcggta ccaagacgtc aactcgacct ccgagacgga
    10921 aagtgaaacg tgatttcatg cgtcattttg aacattttgt aaatcttatt taataatgtg
    10981 tgcggcaatt cacatttaat ttatgaatgt tttcttaaca tcgcggcaac tcaagaaacg
    11041 gcaggttcgg atcttagcta ctagagaaag aggagaaata ctagatgcgt aaaggcgaag
    11101 agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc aacggtcata
    11161 agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt
    11221 tcatctgtac tactggtaaa ctgccggttc cttggccgac tctggtaacg acgctgactt
    11281 atggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac ttcttcaagt
    11341 ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat gacggcacgt
    11401 acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc attgagctga
    11461 aaggcattga ctttaaagag gacggcaata tcctgggcca taagctggaa tacaatttta
    11521 acagccacaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa gcgaatttta
    11581 aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac cagcaaaaca
    11641 ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc acgcaaagcg
    11701 ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag ttcgtaaccg
    11761 cagcgggcat cacgcatggt atggatgaac tgta
//
