LOCUS       sequence-429167-        7818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral transduction of EGFP-tagged STING.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Chu TT, Tu X, Yang K, Wu J, Repa JJ, Yan N
  TITLE     Tonic prime-boost of STING signalling mediates Niemann-Pick disease 
            type C.
  JOURNAL   Nature. 2021 Aug;596(7873):570-575. doi: 10.1038/s41586-021-03762-2.
            Epub 2021 Jul 21.
  PUBMED    34290407
REFERENCE   2  (bases 1 to 7818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03762-2"; journalName: "Nature"; date: 
            "2021-08"; volume: "596"; issue: "7873"; pages: "570-575"
COMMENT     Sequence Label: pMRX-hSTING-EGFP
FEATURES             Location/Qualifiers
     source          1..7818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    315..672
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     enhancer        1031..1410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1512..1530)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1598..1702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1703..1771,1772..2563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1921..1940)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2727..3315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3216..3235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3469..3486
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             3616..4085
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4689..5238
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4848..4867)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5077..5094
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(5373..6086,6087..6089,6090..6092))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5376..6086,6087..6089,6090..6092))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5418..5439)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5767..5786
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6026..6047
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6089..6098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7268..7642
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(7314..7330)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(join(7652..7818,1..250))
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(7668..7684)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7700..7722)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tcacgatgta ggggacctgg tctgggtgtc catgcgggcc aggtgaaaag accttgatct
       61 taacctgggt gatgaggtct cggttaaagg tgccgtctcg cggccatccg acgttaaagg
      121 ttggccattc tgcagagcag aaggtaaccc aacgtctctt cttgacatct accgactggt
      181 tgtgagcgat ccgctcgaca tctttccagt gacctaaggt caaacttaag ggagtggtaa
      241 cagtctggcc cgggcccata ttttcagaca aatacagaaa cacagtcaga cagagacaac
      301 acagaacgat gctgcagcag acaagacgcg cggcgcggct tcggtcccaa accgaaagca
      361 aaaattcaga cggaggcggg aactgtttta ggttctcgtc tcctaccaga accacatatc
      421 cctcctctaa ggggggtgca ccaaagagtc caaaacgatc gggatttttg gactcaggtc
      481 gggccacaaa aacggccccc gaagtccctg ggacgtctcc cagggttgcg gccgggtgtt
      541 ccgaactcgt cagttccacc acgggtccgc cagatacaga gctagttagc taactagtac
      601 cgacgcaggc gcataaaatc agtcatagac actagacaat cggacagaca cagataagtt
      661 gctggccagc ttacctcccg gtggtgggtc ggtggtccct gggcaggggt ctcccgatcc
      721 cggacgagcc cccaaatgaa agacccccgc tgacgggtag tcaatcactc agaggagacc
      781 ctcccaagga acagcgagac cacaagtcgg atgcaactgc aagagggttt attggataca
      841 cgggtacccg ggcgactcag tcaatcggag gactggcgcg ccgagtgagg ggttgtgggc
      901 tcttttattg agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg aactgattgg
      961 ttagttcaaa taaggcacag ggtcatttca ggtccttggg gcaccctgga aacatctgat
     1021 ggttctctag catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     1081 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     1141 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     1201 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     1261 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     1321 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     1381 actattaata actagtcaat aatcaatgtc aacatggcgg taatgttgga catgagccaa
     1441 tataaatgta catattatga tatggataca acgtatgcaa tgggccaagc tcctcgagtg
     1501 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     1561 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1621 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1681 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1741 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1801 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1861 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1921 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     1981 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2041 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2101 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2161 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2221 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2281 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2341 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2401 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2461 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2521 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2581 ctcatatata ctttagattg atttgcggcc ggccgcaaaa ggatctaggt gaagatcctt
     2641 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2701 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2761 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2821 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2881 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2941 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3001 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3061 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3121 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3181 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3241 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3301 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3361 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3421 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3481 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3541 cattaatgca actatggcca tttaatgtaa atacttaaga aaaaaaacca aattaatttt
     3601 gatacatgct gcatgtgaaa gacccccgct gacgggtagt caatcactca gaggagaccc
     3661 tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta ttggatacac
     3721 gggtacccgg gcgactcagt caatcggagg actggcgcgc cgagtgaggg gttgtgggct
     3781 cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga actgattggt
     3841 tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa acatctgatg
     3901 gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc ttggccctga
     3961 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc
     4021 tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt ttggcccata
     4081 ttcagctgtt ccatctgttc ctgaccttga tctgaacttc tctattctca gttatgtatt
     4141 tttccatgcc ttgcaaaatg gcgttactta agctagcttg ccaaacctac aggtggggtc
     4201 tttcattccc ccctttttct ggagactaaa taaaatcttt tattttatcg tcgactttta
     4261 atttcgggta tatttgagtg gaatgagttc ttcaatcgta gttttgacta acttgccatt
     4321 catttctatt aacacaaaac aatctggtgc atagtctgaa atcaactccc tacacatacc
     4381 acaaggactt accactcgaa tacttctatc tacttcgtca gaataagggt gtctaacagc
     4441 tacaatcgtg tcaaaatcct tttgtccatt cgaaactgca ctaccaatcg caatggcttc
     4501 tgcacaaaca gttactcgtc ctatatacgc ttcaatatgt actgccgaaa tgatttctcc
     4561 tgttttcgta cgaattgccg ctcccacatg atgtttatta tcctcataaa gcattgtaat
     4621 cttctctgtc gctacttcta ctaattctag atcttgttga gaaatgttaa atgttttcat
     4681 gaccatggta ttatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta
     4741 gacgtttttt taacctcgac taaacacatg taaagcatgt gcaccgaggc cccagatcag
     4801 atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg
     4861 agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg
     4921 cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg
     4981 gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca
     5041 gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg agaggggaaa
     5101 gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc acattgccaa
     5161 aagacggcaa tatggtggaa aataacatat agacaaacgc acaccggcct tattccaagc
     5221 ggcttcggcc agtaacgtta gggggggggg gcggaattga tcccgctaga gggaaaccgt
     5281 tgctagctta agttagttaa caattgttac cgcggccggc atgcatgacc atgattacgc
     5341 caagtatcga ttccacgcgt tcctgcggcc gcttacttgt acagctcgtc catgccgaga
     5401 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
     5461 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
     5521 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
     5581 ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag
     5641 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
     5701 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
     5761 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
     5821 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
     5881 cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
     5941 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
     6001 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
     6061 ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgagagaaa
     6121 tccgtgcgga gagggagggg cttttccatt ccactgatga ggagctcagg ctcttgggac
     6181 atcgtggagg tactgggcac cgctgaggtc ttcaagctgc ccacagtaac ctcttccttt
     6241 tcctcctgcc gcaggtgccg gagaacctcc tgggacagcg agaagctgct gtcatctgca
     6301 ggttcctggt aggcaatgag gcggcagttg ttctgagact caggggcatc tgccaggatg
     6361 tcctcaagtg tccggcagaa gagtttggcc tgctcaagcc tatcctcccg gctaaagcca
     6421 gcttgactgt attgtgacat ggcaaacaaa gtctgcaagg gggtggcgta ctccaggaca
     6481 caggtgcccg cccgctgccc gttctccaga agctcataga tgctgttgct gtaaacccga
     6541 tccttgatgc cagcacggtc accggtctgc tggggcagtt tatccaggaa gcgaatgttg
     6601 gggtcagcca tactcaggtt atcaggcacc ccacagtcca atgggaggag aatatacagc
     6661 cgctggctca ctgcaccccg tagcaggttg ttgtaatgct gattgtaagt tcgaatccgg
     6721 gcctggagct ctggcaggat cagccgcaga tatccgatgt aatatgacca tgccagccca
     6781 tgggccacgt tgaaattccc tttttcacac actgcagaga tctcagctgg ggccaggccc
     6841 ttgaggccca ggaggatgtt cagtgcctgc gagaggccca ggagggcaag catccaagtg
     6901 aagggcgggc cgaccgcatt tgggagggag tagtagaaat agatggacag cagcaacagg
     6961 gccccacggc ggagggggca gcccaggcag gcccgcacag tcctccagta gctgccccgg
     7021 tacctggagt ggatgtggcg cagctcctca gccaggctgc agaccccgtt taacagcagt
     7081 cccagctgca gggaggctag gtggagcacc aggtaccgga gagtgtgctc tggtggctct
     7141 cctagccccc aaagggtcac caggcaggca ctcagcagaa ccaaggctgc cttctgggcc
     7201 ccgtgacccc tgggacacgg gatggatgga tgcaggctgg agtggggcat gaattctccg
     7261 gatccggcag tctagaggat ggtccacccc cggggtcggc agccttcacg tgggcggcgt
     7321 gtatccaagc tgcgatgccg tctactttga gggcggtggg ggtggtcagc aggactgtgt
     7381 aaggtccttt ccagcgaggt tctaggttct tagtctggtg tcggcggacc cacactgtgt
     7441 cgccgactcg gtaagggtga ggtaccaccg gtcggtccag ttgttcttgg taggctgccg
     7501 ccagaggtct ccagacttcg tgctggacta agtagagagc ctgtaagtga gcttggagag
     7561 aggggctgtt agtaactctt gtcatgtcag ggtcagggaa gtttacaagg ggcgggggtg
     7621 ccccatataa gatctcatat ggccatatgg gggcgcctag agaaggagtg agggctggat
     7681 aaagggagga tcgaggcggg gtcgaacgag gaggttcaag ggggagagac ggggcggatg
     7741 gaggaagagg aggcggaggc ttagggtgta caaagggctt gacccaggga ggggggtcaa
     7801 aagccaaggc ttcccagg
//
