LOCUS       sequence-429171-        7791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral transduction of EGFP-tagged STING.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Chu TT, Tu X, Yang K, Wu J, Repa JJ, Yan N
  TITLE     Tonic prime-boost of STING signalling mediates Niemann-Pick disease 
            type C.
  JOURNAL   Nature. 2021 Aug;596(7873):570-575. doi: 10.1038/s41586-021-03762-2.
            Epub 2021 Jul 21.
  PUBMED    34290407
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03762-2"; journalName: "Nature"; date: 
            "2021-08"; volume: "596"; issue: "7873"; pages: "570-575"
COMMENT     Sequence Label: pMRX-mSTING-EGFP
FEATURES             Location/Qualifiers
     source          1..7791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    302..659
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     enhancer        1018..1397
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1499..1517)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1585..1689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1690..1758,1759..2550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1908..1927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2714..3302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3203..3222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3456..3473
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             3603..4072
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4676..5225
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4835..4854)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5064..5081
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(5308..6021,6022..6024,6025..6027))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5311..6021,6022..6024,6025..6027))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5353..5374)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5702..5721
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5961..5982
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6024..6033
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7228..7602
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(7274..7290)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(join(7612..7791,1..237))
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(7628..7644)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7660..7682)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gacctggtct gggtgtccat gcgggccagg tgaaaagacc ttgatcttaa cctgggtgat
       61 gaggtctcgg ttaaaggtgc cgtctcgcgg ccatccgacg ttaaaggttg gccattctgc
      121 agagcagaag gtaacccaac gtctcttctt gacatctacc gactggttgt gagcgatccg
      181 ctcgacatct ttccagtgac ctaaggtcaa acttaaggga gtggtaacag tctggcccgg
      241 gcccatattt tcagacaaat acagaaacac agtcagacag agacaacaca gaacgatgct
      301 gcagcagaca agacgcgcgg cgcggcttcg gtcccaaacc gaaagcaaaa attcagacgg
      361 aggcgggaac tgttttaggt tctcgtctcc taccagaacc acatatccct cctctaaggg
      421 gggtgcacca aagagtccaa aacgatcggg atttttggac tcaggtcggg ccacaaaaac
      481 ggcccccgaa gtccctggga cgtctcccag ggttgcggcc gggtgttccg aactcgtcag
      541 ttccaccacg ggtccgccag atacagagct agttagctaa ctagtaccga cgcaggcgca
      601 taaaatcagt catagacact agacaatcgg acagacacag ataagttgct ggccagctta
      661 cctcccggtg gtgggtcggt ggtccctggg caggggtctc ccgatcccgg acgagccccc
      721 aaatgaaaga cccccgctga cgggtagtca atcactcaga ggagaccctc ccaaggaaca
      781 gcgagaccac aagtcggatg caactgcaag agggtttatt ggatacacgg gtacccgggc
      841 gactcagtca atcggaggac tggcgcgccg agtgaggggt tgtgggctct tttattgagc
      901 tcggggagca gaagcgcgcg aacagaagcg agaagcgaac tgattggtta gttcaaataa
      961 ggcacagggt catttcaggt ccttggggca ccctggaaac atctgatggt tctctagcat
     1021 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     1081 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     1141 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     1201 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     1261 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     1321 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     1381 agtcaataat caatgtcaac atggcggtaa tgttggacat gagccaatat aaatgtacat
     1441 attatgatat ggatacaacg tatgcaatgg gccaagctcc tcgagtgaag acgaaagggc
     1501 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     1561 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1621 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1681 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1741 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1801 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1861 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1921 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1981 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     2041 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     2101 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2161 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2221 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2281 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2341 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2401 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2461 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2521 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2581 tagattgatt tgcggccggc cgcaaaagga tctaggtgaa gatccttttt gataatctca
     2641 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2701 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2761 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2821 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2881 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2941 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3001 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3061 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3361 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3421 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3481 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcaact
     3541 atggccattt aatgtaaata cttaagaaaa aaaaccaaat taattttgat acatgctgca
     3601 tgtgaaagac ccccgctgac gggtagtcaa tcactcagag gagaccctcc caaggaacag
     3661 cgagaccaca agtcggatgc aactgcaaga gggtttattg gatacacggg tacccgggcg
     3721 actcagtcaa tcggaggact ggcgccccga gtgaggggtt gtgggctctt ttattgagct
     3781 cggggagcag aagcgcgcga acagaagcga gaagcgaact gattggttag ttcaaataag
     3841 gcacagggtc atttcaggtc cttggggcac cctggaaaca tctgatggtt ctctagaaac
     3901 tgctgagggc tggaccgcat ctggggacca tctgttcttg gccctgagcc ggggcaggaa
     3961 ctgcttacca cagatatcct gtttggccca tattcagctg ttccatctgt tcttggccct
     4021 gagccggggc aggaactgct taccacagat atcctgtttg gcccatattc agctgttcca
     4081 tctgttcctg accttgatct gaacttctct attctcagtt atgtattttt ccatgccttg
     4141 caaaatggcg ttacttaagc tagcttgcca aacctacagg tggggtcttt cattcccccc
     4201 tttttctgga gactaaataa aatcttttat tttatcgtcg acttttaatt tcgggtatat
     4261 ttgagtggaa tgagttcttc aatcgtagtt ttgactaact tgccattcat ttctattaac
     4321 acaaaacaat ctggtgcata gtctgaaatc aactccctac acataccaca aggacttacc
     4381 actcgaatac ttctatctac ttcgtcagaa taagggtgtc taacagctac aatcgtgtca
     4441 aaatcctttt gtccattcga aactgcacta ccaatcgcaa tggcttctgc acaaacagtt
     4501 actcgtccta tatacgcttc aatatgtact gccgaaatga tttctcctgt tttcgtacga
     4561 attgccgctc ccacatgatg tttattatcc tcataaagca ttgtaatctt ctctgtcgct
     4621 acttctacta attctagatc ttgttgagaa atgttaaatg ttttcatgac catggtatta
     4681 tcgtgttttt caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa
     4741 cctcgactaa acacatgtaa agcatgtgca ccgaggcccc agatcagatc ccatacaatg
     4801 gggtaccttc tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac
     4861 tctttccaca actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt
     4921 atcttataca cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg
     4981 cctgcaaagg gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc
     5041 cttcacgaca ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat
     5101 gctcgtcaag aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat
     5161 ggtggaaaat aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt
     5221 aacgttaggg ggggggggcg gaattgatcc cgctagaggg aaaccgttgc tagcttaagt
     5281 tagttaacaa ttgttaccgc ggccgcttta cttgtacagc tcgtccatgc cgagagtgat
     5341 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
     5401 gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
     5461 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
     5521 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
     5581 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
     5641 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
     5701 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
     5761 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
     5821 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
     5881 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
     5941 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
     6001 gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg cccgcgggat
     6061 gaggtcagtg cggagtggga gaggctgatc cataccactg atgaggagtc ttggctcttg
     6121 ggacagtacg gagggaggag gtgccactga ggtcatgggg gcattcatgg taacctcctc
     6181 cttttcttcc tgacgaatgt gccggagcac ctcctgagac agtgagaaac tgtttccgtc
     6241 tgtgggttct tggtagacaa tgaggcggca gttatttcga gactcgggga catcttccag
     6301 gatttcctca agtgtccggc agaagagttt agcctgctca agccgatcct cccgactgaa
     6361 gccagctttg gcatcctgtg acatggcaaa cagggtctgc aagggggtgg cgtactccag
     6421 gatacagacg cctgctggct gtccgttctc cagaatctcg tagacgctgt tggaataaac
     6481 ccgattcttg atgccagcac ggtcgatgtt ttgctggggc agcatatctc ggaatcgaat
     6541 gttggggtca actacactca ggttgtcagg caccccacag tccaatggaa agaggatgta
     6601 cagtcttcgg ctccctgcac cactgagcat gttgttatgt agctgattga acattcggat
     6661 ccgggcctgg agccctggta agatcaaccg caagtaccca atgtagtatg accaggccag
     6721 cccgtgggca acatttaact tcttttcttc acagactgca gagacttccg ctggagtcaa
     6781 gctctgaagg cccaggagca tgcttaggga cttatagagg accagaaggc caaacatcca
     6841 actgaggtat atgtcagcag tgttttggag gaaatagaaa taagacgata gtagaatcat
     6901 agccatacag tggatggggc atcccaggca ggcgcgcaca gccttccagt agctgccctg
     6961 gtacctggac tggacatggc acagctcttc agccagacag cagaggtttt tcaacagtag
     7021 tccaagttcg tgcgaggcta ggtgaagtgc taggtacttc agagtgtgat ttggtggatc
     7081 ctttgccacc caaaggatca tcaggctggc caccagaaag atgagggcta catatttgga
     7141 gcggtgacct ctgggccgtg ggatggctgg atgcaggttg gagtatggca tggtggcgaa
     7201 ttcccgtacc accacactgg gatccggcag tctagaggat ggtccacccc cggggtcggc
     7261 agccttcacg tgggcggcgt gtatccaagc tgcgatgccg tctactttga gggcggtggg
     7321 ggtggtcagc aggactgtgt aaggtccttt ccagcgaggt tctaggttct tagtctggtg
     7381 tcggcggacc cacactgtgt cgccgactcg gtaagggtga ggtaccaccg gtcggtccag
     7441 ttgttcttgg taggctgccg ccagaggtct ccagacttcg tgctggacta agtagagagc
     7501 ctgtaagtga gcttggagag aggggctgtt agtaactctt gtcatgtcag ggtcagggaa
     7561 gtttacaagg ggcgggggtg ccccatataa gatctcatat ggccatatgg gggcgcctag
     7621 agaaggagtg agggctggat aaagggagga tcgaggcggg gtcgaacgag gaggttcaag
     7681 ggggagagac ggggcggatg gaggaagagg aggcggaggc ttagggtgta caaagggctt
     7741 gacccaggga ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg g
//
