LOCUS       sequence-429172-        7110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral transduction of STING.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Wu J, Dobbs N, Yang K, Yan N
  TITLE     Interferon-Independent Activities of Mammalian STING Mediate 
            Antiviral Response and Tumor Immune Evasion.
  JOURNAL   Immunity. 2020 Jul 14;53(1):115-126.e5. doi: 
            10.1016/j.immuni.2020.06.009. Epub 2020 Jul 7.
  PUBMED    32640258
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2020.06.009"; journalName: "Immunity"; date: 
            "2020-07-14- 14"; volume: "53"; issue: "1"; pages: "115-126.e5"
COMMENT     Sequence Label: pMRX-HA-hSTING (S366A)
FEATURES             Location/Qualifiers
     source          1..7110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        677..1056
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1158..1176)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1244..1348
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1349..1417,1418..2209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1567..1586)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2373..2961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2862..2881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3115..3132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             3262..3731
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4335..4884
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4494..4513)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4723..4740
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(6168..6194)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6206..6580
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(6252..6268)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(6590..7006)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(6606..6622)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6638..6660)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    join(7071..7110,1..318)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 aaagcaaaaa ttcagacgga ggcgggaact gttttaggtt ctcgtctcct accagaacca
       61 catatccctc ctctaagggg ggtgcaccaa agagtccaaa acgatcggga tttttggact
      121 caggtcgggc cacaaaaacg gcccccgaag tccctgggac gtctcccagg gttgcggccg
      181 ggtgttccga actcgtcagt tccaccacgg gtccgccaga tacagagcta gttagctaac
      241 tagtaccgac gcaggcgcat aaaatcagtc atagacacta gacaatcgga cagacacaga
      301 taagttgctg gccagcttac ctcccggtgg tgggtcggtg gtccctgggc aggggtctcc
      361 cgatcccgga cgagccccca aatgaaagac ccccgctgac gggtagtcaa tcactcagag
      421 gagaccctcc caaggaacag cgagaccaca agtcggatgc aactgcaaga gggtttattg
      481 gatacacggg tacccgggcg actcagtcaa tcggaggact ggcgcgccga gtgaggggtt
      541 gtgggctctt ttattgagct cggggagcag aagcgcgcga acagaagcga gaagcgaact
      601 gattggttag ttcaaataag gcacagggtc atttcaggtc cttggggcac cctggaaaca
      661 tctgatggtt ctctagcatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
      721 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
      781 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
      841 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
      901 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
      961 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     1021 ttgattacta ttaataacta gtcaataatc aatgtcaaca tggcggtaat gttggacatg
     1081 agccaatata aatgtacata ttatgatatg gatacaacgt atgcaatggg ccaagctcct
     1141 cgagtgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     1201 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1261 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1321 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1381 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1441 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1501 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1561 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     1621 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1681 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1741 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1801 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1861 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1921 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1981 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     2041 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     2101 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     2161 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     2221 agtttactca tatatacttt agattgattt gcggccggcc gcaaaaggat ctaggtgaag
     2281 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2341 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2761 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2881 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3001 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3061 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3121 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3181 ccgattcatt aatgcaacta tggccattta atgtaaatac ttaagaaaaa aaaccaaatt
     3241 aattttgata catgctgcat gtgaaagacc cccgctgacg ggtagtcaat cactcagagg
     3301 agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag ggtttattgg
     3361 atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag tgaggggttg
     3421 tgggctcttt tattgagctc ggggagcaga agcgcgcgaa cagaagcgag aagcgaactg
     3481 attggttagt tcaaataagg cacagggtca tttcaggtcc ttggggcacc ctggaaacat
     3541 ctgatggttc tctagaaact gctgagggct ggaccgcatc tggggaccat ctgttcttgg
     3601 ccctgagccg gggcaggaac tgcttaccac agatatcctg tttggcccat attcagctgt
     3661 tccatctgtt cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg
     3721 cccatattca gctgttccat ctgttcctga ccttgatctg aacttctcta ttctcagtta
     3781 tgtatttttc catgccttgc aaaatggcgt tacttaagct agcttgccaa acctacaggt
     3841 ggggtctttc attcccccct ttttctggag actaaataaa atcttttatt ttatcgtcga
     3901 cttttaattt cgggtatatt tgagtggaat gagttcttca atcgtagttt tgactaactt
     3961 gccattcatt tctattaaca caaaacaatc tggtgcatag tctgaaatca actccctaca
     4021 cataccacaa ggacttacca ctcgaatact tctatctact tcgtcagaat aagggtgtct
     4081 aacagctaca atcgtgtcaa aatccttttg tccattcgaa actgcactac caatcgcaat
     4141 ggcttctgca caaacagtta ctcgtcctat atacgcttca atatgtactg ccgaaatgat
     4201 ttctcctgtt ttcgtacgaa ttgccgctcc cacatgatgt ttattatcct cataaagcat
     4261 tgtaatcttc tctgtcgcta cttctactaa ttctagatct tgttgagaaa tgttaaatgt
     4321 tttcatgacc atggtattat cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg
     4381 ggcctagacg tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac cgaggcccca
     4441 gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg ttgaatacgc
     4501 ttgaggagag ccatttgact ctttccacaa ctatccaact cacaacgtgg cactggggtt
     4561 gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg cacctgtcgc
     4621 caggtggggg gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag
     4681 cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct ttggcgagag
     4741 gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg gccctcacat
     4801 tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac cggccttatt
     4861 ccaagcggct tcggccagta acgttagggg gggggggcgg aattgatccc gctagaggga
     4921 aaccgttgct agcttaagtt agttaacaat tgttaccgcg gccggcatgc atgaccatga
     4981 ttacgccaag tatcgattcc acgcgttcct gcggccgctc cgaattctca agagaaatcc
     5041 gtgcggagag ggaggggctt ttccattcca gcgatgagga gctcaggctc ttgggacatc
     5101 gtggaggtac tgggcaccgc tgaggtcttc aagctgccca cagtaacctc ttccttttcc
     5161 tcctgccgca ggtgccggag aacctcctgg gacagcgaga agctgctgtc atctgcaggt
     5221 tcctggtagg caatgaggcg gcagttgttc tgagactcag gggcatctgc caggatgtcc
     5281 tcaagtgtcc ggcagaagag tttggcctgc tcaagcctat cctcccggct aaagccagct
     5341 tgactgtatt gtgacatggc aaacaaagtc tgcaaggggg tggcgtactc caggacacag
     5401 gtgcccgccc gctgcccgtt ctccagaagc tcatagatgc tgttgctgta aacccgatcc
     5461 ttgatgccag cacggtcacc ggtctgctgg ggcagtttat ccaggaagcg aatgttgggg
     5521 tcagccatac tcaggttatc aggcacccca cagtccaatg ggaggagaat atacagccgc
     5581 tggctcactg caccccgtag caggttgttg taatgctgat tgtaagttcg aatccgggcc
     5641 tggagctctg gcaggatcag ccgcagatat ccgatgtaat atgaccatgc cagcccatgg
     5701 gccacgttga aattcccttt ttcacacact gcagagatct cagctggggc caggcccttg
     5761 aggcccagga ggatgttcag tgcctgcgag aggcccagga gggcaagcat ccaagtgaag
     5821 ggcgggccga ccgcatttgg gagggagtag tagaaataga tggacagcag caacagggcc
     5881 ccacggcgga gggggcagcc caggcaggcc cgcacagtcc tccagtagct gccccggtac
     5941 ctggagtgga tgtggcgcag ctcctcagcc aggctgcaga ccccgtttaa cagcagtccc
     6001 agctgcaggg aggctaggtg gagcaccagg taccggagag tgtgctctgg tggctctcct
     6061 agcccccaaa gggtcaccag gcaggcactc agcagaacca aggctgcctt ctgggccccg
     6121 tgacccctgg gacacgggat ggatggatgc aggctggagt ggggcatagc gtaatctggt
     6181 acgtcgtatg ggtacatgga tccggcagtc tagaggatgg tccacccccg gggtcggcag
     6241 ccttcacgtg ggcggcgtgt atccaagctg cgatgccgtc tactttgagg gcggtggggg
     6301 tggtcagcag gactgtgtaa ggtcctttcc agcgaggttc taggttctta gtctggtgtc
     6361 ggcggaccca cactgtgtcg ccgactcggt aagggtgagg taccaccggt cggtccagtt
     6421 gttcttggta ggctgccgcc agaggtctcc agacttcgtg ctggactaag tagagagcct
     6481 gtaagtgagc ttggagagag gggctgttag taactcttgt catgtcaggg tcagggaagt
     6541 ttacaagggg cgggggtgcc ccatataaga tctcatatgg ccatatgggg gcgcctagag
     6601 aaggagtgag ggctggataa agggaggatc gaggcggggt cgaacgagga ggttcaaggg
     6661 ggagagacgg ggcggatgga ggaagaggag gcggaggctt agggtgtaca aagggcttga
     6721 cccagggagg ggggtcaaaa gccaaggctt cccaggtcac gatgtagggg acctggtctg
     6781 ggtgtccatg cgggccaggt gaaaagacct tgatcttaac ctgggtgatg aggtctcggt
     6841 taaaggtgcc gtctcgcggc catccgacgt taaaggttgg ccattctgca gagcagaagg
     6901 taacccaacg tctcttcttg acatctaccg actggttgtg agcgatccgc tcgacatctt
     6961 tccagtgacc taaggtcaaa cttaagggag tggtaacagt ctggcccggg cccatatttt
     7021 cagacaaata cagaaacaca gtcagacaga gacaacacag aacgatgctg cagcagacaa
     7081 gacgcgcggc gcggcttcgg tcccaaaccg
//
