LOCUS       sequence-429173-        6960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral transduction of STING.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Wu J, Chen YJ, Dobbs N, Sakai T, Liou J, Miner JJ, Yan N
  TITLE     STING-mediated disruption of calcium homeostasis chronically 
            activates ER stress and primes T cell death.
  JOURNAL   J Exp Med. 2019 Apr 1;216(4):867-883. doi: 10.1084/jem.20182192. 
            Epub 2019 Mar 18.
  PUBMED    30886058
REFERENCE   2  (bases 1 to 6960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20182192"; journalName: "J Exp Med"; date: "2019-04-1- 
            1"; volume: "216"; issue: "4"; pages: "867-883"
COMMENT     Sequence Label: pMRX-hSTING-dCTT
FEATURES             Location/Qualifiers
     source          1..6960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        602..981
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1083..1101)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1169..1273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1274..1342,1343..2134)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1492..1511)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2298..2886
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2787..2806
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3040..3057
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             3187..3656
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4260..4809
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4419..4438)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4648..4665
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     misc_feature    5981..6355
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(6027..6043)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(6365..6781)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(6381..6397)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6413..6435)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    join(6846..6960,1..243)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 aggggggtgc accaaagagt ccaaaacgat cgggattttt ggactcaggt cgggccacaa
       61 aaacggcccc cgaagtccct gggacgtctc ccagggttgc ggccgggtgt tccgaactcg
      121 tcagttccac cacgggtccg ccagatacag agctagttag ctaactagta ccgacgcagg
      181 cgcataaaat cagtcataga cactagacaa tcggacagac acagataagt tgctggccag
      241 cttacctccc ggtggtgggt cggtggtccc tgggcagggg tctcccgatc ccggacgagc
      301 ccccaaatga aagacccccg ctgacgggta gtcaatcact cagaggagac cctcccaagg
      361 aacagcgaga ccacaagtcg gatgcaactg caagagggtt tattggatac acgggtaccc
      421 gggcgactca gtcaatcgga ggactggcgc gccgagtgag gggttgtggg ctcttttatt
      481 gagctcgggg agcagaagcg cgcgaacaga agcgagaagc gaactgattg gttagttcaa
      541 ataaggcaca gggtcatttc aggtccttgg ggcaccctgg aaacatctga tggttctcta
      601 gcatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
      661 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
      721 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
      781 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
      841 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
      901 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
      961 aactagtcaa taatcaatgt caacatggcg gtaatgttgg acatgagcca atataaatgt
     1021 acatattatg atatggatac aacgtatgca atgggccaag ctcctcgagt gaagacgaaa
     1081 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     1141 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1201 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1261 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1321 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1381 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1441 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1501 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     1561 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1621 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1681 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1741 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1801 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1861 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1921 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1981 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2041 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2101 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2161 actttagatt gatttgcggc cggccgcaaa aggatctagg tgaagatcct ttttgataat
     2221 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2281 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2341 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2401 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2461 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2521 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2581 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2641 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2701 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2761 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2821 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2881 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2941 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3001 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3061 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3121 aactatggcc atttaatgta aatacttaag aaaaaaaacc aaattaattt tgatacatgc
     3181 tgcatgtgaa agacccccgc tgacgggtag tcaatcactc agaggagacc ctcccaagga
     3241 acagcgagac cacaagtcgg atgcaactgc aagagggttt attggataca cgggtacccg
     3301 ggcgactcag tcaatcggag gactggcgcg ccgagtgagg ggttgtgggc tcttttattg
     3361 agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa
     3421 taaggcacag ggtcatttca ggtccttggg gcaccctgga aacatctgat ggttctctag
     3481 aaactgctga gggctggacc gcatctgggg accatctgtt cttggccctg agccggggca
     3541 ggaactgctt accacagata tcctgtttgg cccatattca gctgttccat ctgttcttgg
     3601 ccctgagccg gggcaggaac tgcttaccac agatatcctg tttggcccat attcagctgt
     3661 tccatctgtt cctgaccttg atctgaactt ctctattctc agttatgtat ttttccatgc
     3721 cttgcaaaat ggcgttactt aagctagctt gccaaaccta caggtggggt ctttcattcc
     3781 cccctttttc tggagactaa ataaaatctt ttattttatc gtcgactttt aatttcgggt
     3841 atatttgagt ggaatgagtt cttcaatcgt agttttgact aacttgccat tcatttctat
     3901 taacacaaaa caatctggtg catagtctga aatcaactcc ctacacatac cacaaggact
     3961 taccactcga atacttctat ctacttcgtc agaataaggg tgtctaacag ctacaatcgt
     4021 gtcaaaatcc ttttgtccat tcgaaactgc actaccaatc gcaatggctt ctgcacaaac
     4081 agttactcgt cctatatacg cttcaatatg tactgccgaa atgatttctc ctgttttcgt
     4141 acgaattgcc gctcccacat gatgtttatt atcctcataa agcattgtaa tcttctctgt
     4201 cgctacttct actaattcta gatcttgttg agaaatgtta aatgttttca tgaccatggt
     4261 attatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct agacgttttt
     4321 ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg ccccagatca gatcccatac
     4381 aatggggtac cttctgggca tccttcagcc ccttgttgaa tacgcttgag gagagccatt
     4441 tgactctttc cacaactatc caactcacaa cgtggcactg gggttgtgcc gcctttgcag
     4501 gtgtatctta tacacgtggc ttttggccgc agaggcacct gtcgccaggt ggggggttcc
     4561 gctgcctgca aagggtcgct acagacgttg tttgtcttca agaagcttcc agaggaactg
     4621 cttccttcac gacattcaac agaccttgca ttcctttggc gagaggggaa agacccctag
     4681 gaatgctcgt caagaagaca gggccaggtt tccgggccct cacattgcca aaagacggca
     4741 atatggtgga aaataacata tagacaaacg cacaccggcc ttattccaag cggcttcggc
     4801 cagtaacgtt aggggggggg ggcggaattg atcccgctag agggaaaccg ttgctagctt
     4861 aagttagtta acaattgtta ccgcggccgg catgcatgac catgattacg ccaagtatcg
     4921 attccacgcg ttcctgcggc cgctcattcc ttttcctcct gccgcaggtg ccggagaacc
     4981 tcctgggaca gcgagaagct gctgtcatct gcaggttcct ggtaggcaat gaggcggcag
     5041 ttgttctgag actcaggggc atctgccagg atgtcctcaa gtgtccggca gaagagtttg
     5101 gcctgctcaa gcctatcctc ccggctaaag ccagcttgac tgtattgtga catggcaaac
     5161 aaagtctgca agggggtggc gtactccagg acacaggtgc ccgcccgctg cccgttctcc
     5221 agaagctcat agatgctgtt gctgtaaacc cgatccttga tgccagcacg gtcaccggtc
     5281 tgctggggca gtttatccag gaagcgaatg ttggggtcag ccatactcag gttatcaggc
     5341 accccacagt ccaatgggag gagaatatac agccgctggc tcactgcacc ccgtagcagg
     5401 ttgttgtaat gctgattgta agttcgaatc cgggcctgga gctctggcag gatcagccgc
     5461 agatatccga tgtaatatga ccatgccagc ccatgggcca cgttgaaatt ccctttttca
     5521 cacactgcag agatctcagc tggggccagg cccttgaggc ccaggaggat gttcagtgcc
     5581 tgcgagaggc ccaggagggc aagcatccaa gtgaagggcg ggccgaccgc atttgggagg
     5641 gagtagtaga aatagatgga cagcagcaac agggccccac ggcggagggg gcagcccagg
     5701 caggcccgca cagtcctcca gtagctgccc cggtacctgg agtggatgtg gcgcagctcc
     5761 tcagccaggc tgcagacccc gtttaacagc agtcccagct gcagggaggc taggtggagc
     5821 accaggtacc ggagagtgtg ctctggtggc tctcctagcc cccaaagggt caccaggcag
     5881 gcactcagca gaaccaaggc tgccttctgg gccccgtgac ccctgggaca cgggatggat
     5941 ggatgcaggc tggagtgggg catgaattct ccggatccgg cagtctagag gatggtccac
     6001 ccccggggtc ggcagccttc acgtgggcgg cgtgtatcca agctgcgatg ccgtctactt
     6061 tgagggcggt gggggtggtc agcaggactg tgtaaggtcc tttccagcga ggttctaggt
     6121 tcttagtctg gtgtcggcgg acccacactg tgtcgccgac tcggtaaggg tgaggtacca
     6181 ccggtcggtc cagttgttct tggtaggctg ccgccagagg tctccagact tcgtgctgga
     6241 ctaagtagag agcctgtaag tgagcttgga gagaggggct gttagtaact cttgtcatgt
     6301 cagggtcagg gaagtttaca aggggcgggg gtgccccata taagatctca tatggccata
     6361 tgggggcgcc tagagaagga gtgagggctg gataaaggga ggatcgaggc ggggtcgaac
     6421 gaggaggttc aagggggaga gacggggcgg atggaggaag aggaggcgga ggcttagggt
     6481 gtacaaaggg cttgacccag ggaggggggt caaaagccaa ggcttcccag gtcacgatgt
     6541 aggggacctg gtctgggtgt ccatgcgggc caggtgaaaa gaccttgatc ttaacctggg
     6601 tgatgaggtc tcggttaaag gtgccgtctc gcggccatcc gacgttaaag gttggccatt
     6661 ctgcagagca gaaggtaacc caacgtctct tcttgacatc taccgactgg ttgtgagcga
     6721 tccgctcgac atctttccag tgacctaagg tcaaacttaa gggagtggta acagtctggc
     6781 ccgggcccat attttcagac aaatacagaa acacagtcag acagagacaa cacagaacga
     6841 tgctgcagca gacaagacgc gcggcgcggc ttcggtccca aaccgaaagc aaaaattcag
     6901 acggaggcgg gaactgtttt aggttctcgt ctcctaccag aaccacatat ccctcctcta
//
