LOCUS       sequence-429176-        7080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral transduction of STING.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Wu J, Dobbs N, Yang K, Yan N
  TITLE     Interferon-Independent Activities of Mammalian STING Mediate 
            Antiviral Response and Tumor Immune Evasion.
  JOURNAL   Immunity. 2020 Jul 14;53(1):115-126.e5. doi: 
            10.1016/j.immuni.2020.06.009. Epub 2020 Jul 7.
  PUBMED    32640258
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2020.06.009"; journalName: "Immunity"; date: 
            "2020-07-14- 14"; volume: "53"; issue: "1"; pages: "115-126.e5"
COMMENT     Sequence Label: pMRX-hSTING (S366A)
FEATURES             Location/Qualifiers
     source          1..7080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        662..1041
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1143..1161)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1229..1333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1334..1402,1403..2194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1552..1571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2358..2946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2847..2866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3100..3117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             3247..3716
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4320..4869
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4479..4498)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4708..4725
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     misc_feature    6161..6535
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(6207..6223)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(6545..6961)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(6561..6577)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6593..6615)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    join(7026..7080,1..303)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 acggaggcgg gaactgtttt aggttctcgt ctcctaccag aaccacatat ccctcctcta
       61 aggggggtgc accaaagagt ccaaaacgat cgggattttt ggactcaggt cgggccacaa
      121 aaacggcccc cgaagtccct gggacgtctc ccagggttgc ggccgggtgt tccgaactcg
      181 tcagttccac cacgggtccg ccagatacag agctagttag ctaactagta ccgacgcagg
      241 cgcataaaat cagtcataga cactagacaa tcggacagac acagataagt tgctggccag
      301 cttacctccc ggtggtgggt cggtggtccc tgggcagggg tctcccgatc ccggacgagc
      361 ccccaaatga aagacccccg ctgacgggta gtcaatcact cagaggagac cctcccaagg
      421 aacagcgaga ccacaagtcg gatgcaactg caagagggtt tattggatac acgggtaccc
      481 gggcgactca gtcaatcgga ggactggcgc gccgagtgag gggttgtggg ctcttttatt
      541 gagctcgggg agcagaagcg cgcgaacaga agcgagaagc gaactgattg gttagttcaa
      601 ataaggcaca gggtcatttc aggtccttgg ggcaccctgg aaacatctga tggttctcta
      661 gcatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
      721 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
      781 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
      841 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
      901 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
      961 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     1021 aactagtcaa taatcaatgt caacatggcg gtaatgttgg acatgagcca atataaatgt
     1081 acatattatg atatggatac aacgtatgca atgggccaag ctcctcgagt gaagacgaaa
     1141 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     1201 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1261 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1321 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1381 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1441 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1501 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1561 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     1621 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1681 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1741 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1801 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1861 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1921 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1981 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2041 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2101 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2161 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2221 actttagatt gatttgcggc cggccgcaaa aggatctagg tgaagatcct ttttgataat
     2281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3001 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3061 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3181 aactatggcc atttaatgta aatacttaag aaaaaaaacc aaattaattt tgatacatgc
     3241 tgcatgtgaa agacccccgc tgacgggtag tcaatcactc agaggagacc ctcccaagga
     3301 acagcgagac cacaagtcgg atgcaactgc aagagggttt attggataca cgggtacccg
     3361 ggcgactcag tcaatcggag gactggcgcc ccgagtgagg ggttgtgggc tcttttattg
     3421 agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa
     3481 taaggcacag ggtcatttca ggtccttggg gcaccctgga aacatctgat ggttctctag
     3541 aaactgctga gggctggacc gcatctgggg accatctgtt cttggccctg agccggggca
     3601 ggaactgctt accacagata tcctgtttgg cccatattca gctgttccat ctgttcttgg
     3661 ccctgagccg gggcaggaac tgcttaccac agatatcctg tttggcccat attcagctgt
     3721 tccatctgtt cctgaccttg atctgaactt ctctattctc agttatgtat ttttccatgc
     3781 cttgcaaaat ggcgttactt aagctagctt gccaaaccta caggtggggt ctttcattcc
     3841 cccctttttc tggagactaa ataaaatctt ttattttatc gtcgactttt aatttcgggt
     3901 atatttgagt ggaatgagtt cttcaatcgt agttttgact aacttgccat tcatttctat
     3961 taacacaaaa caatctggtg catagtctga aatcaactcc ctacacatac cacaaggact
     4021 taccactcga atacttctat ctacttcgtc agaataaggg tgtctaacag ctacaatcgt
     4081 gtcaaaatcc ttttgtccat tcgaaactgc actaccaatc gcaatggctt ctgcacaaac
     4141 agttactcgt cctatatacg cttcaatatg tactgccgaa atgatttctc ctgttttcgt
     4201 acgaattgcc gctcccacat gatgtttatt atcctcataa agcattgtaa tcttctctgt
     4261 cgctacttct actaattcta gatcttgttg agaaatgtta aatgttttca tgaccatggt
     4321 attatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct agacgttttt
     4381 ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg ccccagatca gatcccatac
     4441 aatggggtac cttctgggca tccttcagcc ccttgttgaa tacgcttgag gagagccatt
     4501 tgactctttc cacaactatc caactcacaa cgtggcactg gggttgtgcc gcctttgcag
     4561 gtgtatctta tacacgtggc ttttggccgc agaggcacct gtcgccaggt ggggggttcc
     4621 gctgcctgca aagggtcgct acagacgttg tttgtcttca agaagcttcc agaggaactg
     4681 cttccttcac gacattcaac agaccttgca ttcctttggc gagaggggaa agacccctag
     4741 gaatgctcgt caagaagaca gggccaggtt tccgggccct cacattgcca aaagacggca
     4801 atatggtgga aaataacata tagacaaacg cacaccggcc ttattccaag cggcttcggc
     4861 cagtaacgtt aggggggggg ggcggaattg atcccgctag agggaaaccg ttgctagctt
     4921 aagttagtta acaattgtta ccgcggccgg catgcatgac catgattacg ccaagtatcg
     4981 attccacgcg ttcctgcggc cgctcaagag aaatccgtgc ggagagggag gggcttttcc
     5041 attccagcga tgaggagctc aggctcttgg gacatcgtgg aggtactggg caccgctgag
     5101 gtcttcaagc tgcccacagt aacctcttcc ttttcctcct gccgcaggtg ccggagaacc
     5161 tcctgggaca gcgagaagct gctgtcatct gcaggttcct ggtaggcaat gaggcggcag
     5221 ttgttctgag actcaggggc atctgccagg atgtcctcaa gtgtccggca gaagagtttg
     5281 gcctgctcaa gcctatcctc ccggctaaag ccagcttgac tgtattgtga catggcaaac
     5341 aaagtctgca agggggtggc gtactccagg acacaggtgc ccgcccgctg cccgttctcc
     5401 agaagctcat agatgctgtt gctgtaaacc cgatccttga tgccagcacg gtcaccggtc
     5461 tgctggggca gtttatccag gaagcgaatg ttggggtcag ccatactcag gttatcaggc
     5521 accccacagt ccaatgggag gagaatatac agccgctggc tcactgcacc ccgtagcagg
     5581 ttgttgtaat gctgattgta agttcgaatc cgggcctgga gctctggcag gatcagccgc
     5641 agatatccga tgtaatatga ccatgccagc ccatgggcca cgttgaaatt ccctttttca
     5701 cacactgcag agatctcagc tggggccagg cccttgaggc ccaggaggat gttcagtgcc
     5761 tgcgagaggc ccaggagggc aagcatccaa gtgaagggcg ggccgaccgc atttgggagg
     5821 gagtagtaga aatagatgga cagcagcaac agggccccac ggcggagggg gcagcccagg
     5881 caggcccgca cagtcctcca gtagctgccc cggtacctgg agtggatgtg gcgcagctcc
     5941 tcagccaggc tgcagacccc gtttaacagc agtcccagct gcagggaggc taggtggagc
     6001 accaggtacc ggagagtgtg ctctggtggc tctcctagcc cccaaagggt caccaggcag
     6061 gcactcagca gaaccaaggc tgccttctgg gccccgtgac ccctgggaca cgggatggat
     6121 ggatgcaggc tggagtgggg catgaattct ccggatccgg cagtctagag gatggtccac
     6181 ccccggggtc ggcagccttc acgtgggcgg cgtgtatcca agctgcgatg ccgtctactt
     6241 tgagggcggt gggggtggtc agcaggactg tgtaaggtcc tttccagcga ggttctaggt
     6301 tcttagtctg gtgtcggcgg acccacactg tgtcgccgac tcggtaaggg tgaggtacca
     6361 ccggtcggtc cagttgttct tggtaggctg ccgccagagg tctccagact tcgtgctgga
     6421 ctaagtagag agcctgtaag tgagcttgga gagaggggct gttagtaact cttgtcatgt
     6481 cagggtcagg gaagtttaca aggggcgggg gtgccccata taagatctca tatggccata
     6541 tgggggcgcc tagagaagga gtgagggctg gataaaggga ggatcgaggc ggggtcgaac
     6601 gaggaggttc aagggggaga gacggggcgg atggaggaag aggaggcgga ggcttagggt
     6661 gtacaaaggg cttgacccag ggaggggggt caaaagccaa ggcttcccag gtcacgatgt
     6721 aggggacctg gtctgggtgt ccatgcgggc caggtgaaaa gaccttgatc ttaacctggg
     6781 tgatgaggtc tcggttaaag gtgccgtctc gcggccatcc gacgttaaag gttggccatt
     6841 ctgcagagca gaaggtaacc caacgtctct tcttgacatc taccgactgg ttgtgagcga
     6901 tccgctcgac atctttccag tgacctaagg tcaaacttaa gggagtggta acagtctggc
     6961 ccgggcccat attttcagac aaatacagaa acacagtcag acagagacaa cacagaacga
     7021 tgctgcagca gacaagacgc gcggcgcggc ttcggtccca aaccgaaagc aaaaattcag
//
