LOCUS       sequence-429181-       16618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an endogenous-like L1RP LINE-1 element with a C-terminal 
            mNeonGreen2 fluorophore on the ORF1 protein for fluorescence 
            microscopy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16618)
  AUTHORS   Sil S, Keegan S, Ettefa F, Denes L, Boeke JD, Holt LJ
  TITLE     Condensation of LINE-1 is critical for retrotransposition.
  JOURNAL   Elife. 2023 Apr 28;12:e82991. doi: 10.7554/eLife.82991.
  PUBMED    37114770
REFERENCE   2  (bases 1 to 16618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-04-28"; volume: "12:e82991. doi"; pages: " 
            10.7554/eLife.82991"
COMMENT     Sequence Label: pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2
FEATURES             Location/Qualifiers
     source          1..16618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2246..2255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6778..6912
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7055..7077)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7641..9430
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     promoter        12172..12276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12277..12345,12346..13137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12495..12514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13308..13896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13797..13816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14050..14067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(14148..14167)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14186..14206)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14191..14520
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14371..14506
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             14613..15212
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(14613..14632)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     15109..15129
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    15442..15576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15641..15660)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    15685..15733
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        join(16315..16618,1..11)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    16323..16341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16359..16377
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16394..16412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16430..16448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16466..16484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16501..16519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16537..16555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     join(16602..16618,1..8)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gtcagatcgc ctggagcggc cgctctagcc ctggaatgtg tattaagact gtaaggtggg
       61 ggggaggagc caagatggcc gaataggaac agctccggtc tacagctccc agcgtgagcg
      121 acgcagaaga cggtgatttc tgcatttcca tctgaggtac cgggttcatc tcactaggga
      181 gtgccagaca gtgggcgcag gccagtgtgt gtgcgcaccg tgcgcgagcc gaagcagggc
      241 gaggcattgc ctcacctggg aagcgcaagg ggtcagggag ttccctttcc gagtcaaaga
      301 aaggggtgac ggacgcacct ggaaaatcgg gtcactccca cccgaatatt gcgcttttca
      361 gaccggctta agaaacggcg caccacgaga ctatatcccg cacctggctc ggagggtcct
      421 acgcccacgg aatctcgctg attgctagca cagcagtctg agatcaaact gcaaggcggc
      481 aacgaggctg ggggaggggc gcccgccatt gcccaggctt gcttaggtaa acaaagcagc
      541 agggaagctc gaactgggtg gagcccacca cagctcaagg aggcctgcct gcctctgtag
      601 gctccacctc tgggggcagg gcacagacaa acaaaaagac agcagtaacc tctgcagact
      661 taagtgtccc tgtctgacag ctttgaagag agcagtggtt ctcccagcac gcagctggag
      721 atctgagaac gggcagactg cctcctcaag tgggtccctg acccctgacc cccgagcagc
      781 ctaactggga ggcacccccc agcaggggca cactgacacc tcacacggca gggtattcca
      841 acagacctgc agctgagggt cctgtctgtt agaaggaaaa ctaacaacca gaaaggacat
      901 ctacaccgaa aacccatctg tacatcacca tcatcaaaga ccaaaagtag ataaaaccac
      961 aaagatgggg aaaaaacaga acagaaaaac tggaaactct aaaacgcaga gcgcctctcc
     1021 tcctccaaag gaacgcagtt cctcaccagc aacagaacaa agctggatgg agaatgattt
     1081 tgatgagctg agagaagaag gcttcagacg atcaaattac tctgagctac gggaggacat
     1141 tcaaaccaaa ggcaaagaag ttgaaaactt tgaaaaaaat ttagaagaat gtataactag
     1201 aataaccaat acagagaagt gcttaaagga gctgatggag ctgaaaacca aggctcgaga
     1261 actacgtgaa gaatgcagaa gcctcaggag ccgatgcgat caactggaag aaagggtatc
     1321 agcaatggaa gatgaaatga atgaaatgaa gcgagaaggg aagtttagag aaaaaagaat
     1381 aaaaagaaat gagcaaagcc tccaagaaat atgggactat gtgaaaagac caaatctacg
     1441 tctgattggt gtacctgaaa gtgatgtgga gaatggaacc aagttggaaa acactctgca
     1501 ggatattatc caggagaact tccccaatct agcaaggcag gccaacgttc agattcagga
     1561 aatacagaga acgccacaaa gatactcctc gagaagagca actccaagac acataattgt
     1621 cagattcacc aaagttgaaa tgaaggaaaa aatgttaagg gcagccagag agaaaggtcg
     1681 ggttaccctc aaaggaaagc ccatcagact aacagcggat ctctcggcag aaaccctaca
     1741 agccagaaga gagtgggggc caatattcaa cattcttaaa gaaaagaatt ttcaacccag
     1801 aatttcatat ccagccaaac taagcttcat aagtgaagga gaaataaaat actttataga
     1861 caagcaaatg ttgagagatt ttgtcaccac caggcctgcc ctaaaagagc tcctgaagga
     1921 agcgctaaac atggaaagga acaaccggta ccagccgctg caaaatcatg ccaaaatggg
     1981 aggcggtggg tctgtgagca agggtgagga ggataacatg gcctctctcc cagcgactca
     2041 tgagttacac atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggtac
     2101 cggcaatcca aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca
     2161 gttctccccc tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta
     2221 ccctgacggg atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca
     2281 tcgcacaatg cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga
     2341 gggaagccac atcaaaggag aggcccaggt gatggggact ggtttccctg ctgacggtcc
     2401 tgtgatgacc aacacgctga ccgctgcgga ctggtgcatg tcgaagaaga cttaccccaa
     2461 cgacaaaacc atcatcagta cctttaagtg gagttacacc actgtaaatg gcaaacgcta
     2521 ccggagcact gcgcggacca cctacacctt tgccaagcca atggcggcta actatctgaa
     2581 gaaccagccg atgtacgtgt tccgtaagac ggagctcaag cactccatga ccgagctcaa
     2641 cttcaaggag tggcaaaagg cctttaccga tatgatgtaa agaccatcaa gactaggaag
     2701 aaactgcacc aactaatgag caaaatcacc agctaacatc ataatgacag gatcaacttc
     2761 acacataaca atattaactt taaatataaa tggactaaat tctgcaatta aaagacacag
     2821 actggcaagt tggataaaga gtcaagaccc atcagtgtgc tgtattcagg aaacccatct
     2881 cacgtgcaga gacacacata ggctcaaaat aaaaggatgg aggaagatct accaagccaa
     2941 tggaaaacaa aaaaaggcag gggttgcaat cctagtctct gataaaacag actttaaacc
     3001 aacaaagatc aaaagagaca aagaaggcca ttacataatg gtaaagggat caattcaaca
     3061 agaggagcta actatcctaa atatttatgc acccaataca ggagcaccca gattcataaa
     3121 gcaagtcctc agtgacctac aaagagactt agactcccac acattaataa tgggagactt
     3181 taacacccca ctgtcaacat tagacagatc aacgagacag aaagtcaaca aggataccca
     3241 ggaattgaac tcagctctgc accaagcaga cctaatagac atctacagaa ctctccaccc
     3301 caaatcaaca gaatatacgt ttttttcagc accacaccac acctattcca aaattgacca
     3361 catagttgga agcaaagctc tcctcagcaa atgtaaaaga acagaaatta taacaaacta
     3421 tctctcagac cacagtgcaa tcaaactaga gctcaggatt aagaatctca ctcaaagccg
     3481 ctcaactaca tggaaactga acaacctgct cctgaatgac tactgggtac ataacgaaat
     3541 gaaggcagaa ataaagatgt tctttgaaac caacgagaac aaagacacca cataccagaa
     3601 tctctgggac gcattcaaag cagtatgtag agggaaattt atagcactaa atgcctacaa
     3661 gagaaagcag gaaagatcca aaattgacac cctaacatca caattaaaag aactagaaaa
     3721 gcaagagcaa acacattcaa aagctagcag aaggcaagaa ataactaaaa tcagagcaga
     3781 actgaaggaa atagagacac aaaaaaccct tcaaaaaatc aatgaatcca ggagctggtt
     3841 ttttgaaagg atcaacaaaa ttgatagacc gctagcaaga ctaataaaga aaaaaagaga
     3901 gaagaatcaa atagacacaa taaaaaatga taaaggggat atcaccaccg atcccacaga
     3961 aatacaaact accatcagag aatactacaa acacctctac gcaaataaac tagaaaatct
     4021 agaagaaatg gatacattcc tcgacacata cactctccca agactaaacc aggaagaagt
     4081 tgaatctctg aatcgaccaa taacaggctc tgaaattgtg gcaataatca atagtttacc
     4141 aaccaaaaag agtccaggac cagatggatt cacagccgaa ttctaccaga ggtacaagga
     4201 ggaactggta ccattccttc tgaaactatt ccaatcaata gaaaaagagg gaatcctccc
     4261 taactcattt tatgaggcca gcatcattct gataccaaag ccgggcagag acacaaccaa
     4321 aaaagagaat tttagaccaa tatccttgat gaacattgat gcaaaaatcc tcaataaaat
     4381 actggcaaac cgaatccagc agcacatcaa aaagcttatc caccatgatc aagtgggctt
     4441 catccctggg atgcaaggct ggttcaatat acgcaaatca ataaatgtaa tccagcatat
     4501 aaacagagcc aaagacaaaa accacatgat tatctcaata gatgcagaaa aagcctttga
     4561 caaaattcaa caacccttca tgctaaaaac tctcaataaa ttaggtattg atgggacgta
     4621 tttcaaaata ataagagcta tctatgacaa acccacagcc aatatcatac tgaatgggca
     4681 aaaactggaa gcattccctt tgaaaaccgg cacaagacag ggatgccctc tctcaccgct
     4741 cctattcaac atagtgttgg aagttctggc cagggcaatc aggcaggaga aggaaataaa
     4801 gggtattcaa ttaggaaaag aggaagtcaa attgtccctg tttgcagacg acatgattgt
     4861 ttatctagaa aaccccatcg tctcagccca aaatctcctt aagctgataa gcaacttcag
     4921 caaagtctca ggatacaaaa tcaatgtaca aaaatcacaa gcattcttat acaccaacaa
     4981 cagacaaaca gagagccaaa tcatgggtga actcccattc acaattgctt caaagagaat
     5041 aaaataccta ggaatccaac ttacaaggga tgtgaaggac ctcttcaagg agaactacaa
     5101 accactgctc aaggaaataa aagaggagac aaacaaatgg aagaacattc catgctcatg
     5161 ggtaggaaga atcaatatcg tgaaaatggc catactgccc aaggtaattt acagattcaa
     5221 tgccatcccc atcaagctac caatgacttt cttcacagaa ttggaaaaaa ctactttaaa
     5281 gttcatatgg aaccaaaaaa gagcccgcat tgccaagtca atcctaagcc aaaagaacaa
     5341 agctggaggc atcacactac ctgacttcaa actatactac aaggctacag taaccaaaac
     5401 agcatggtac tggtaccaaa acagagatat agatcaatgg aacagaacag agccctcaga
     5461 aataatgccg catatctaca actatctgat ctttgacaaa cctgagaaaa acaagcaatg
     5521 gggaaaggat tccctattta ataaatggtg ctgggaaaac tggctagcca tatgtagaaa
     5581 gctgaaactg gatcccttcc ttacacctta tacaaaaatc aattcaagat ggattaaaga
     5641 tttaaacgtt aaacctaaaa ccataaaaac cctagaagaa aacctaggca ttaccattca
     5701 ggacataggc gtgggcaagg acttcatgtc caaaacacca aaagcaatgg caacaaaaga
     5761 caaaattgac aaatgggatc taattaaact aaagagcttc tgcacagcaa aagaaactac
     5821 catcagagtg aacaggcaac ctacaacatg ggagaaaatt tttgcaacct actcatctga
     5881 caaagggcta atatccagaa tctacaatga actcaaacaa atttacaaga aaaaaacaaa
     5941 caaccccatc aaaaagtggg cgaaggacat gaacagacac ttctcaaaag aagacattta
     6001 tgcagccaaa aaacacatga agaaatgctc atcatcactg gccatcagag aaatgcaaat
     6061 caaaaccact atgagatatc atctcacacc agttagaatg gcaatcatta aaaagtcagg
     6121 aaacaacagg tgctggagag gatgcggaga aataggaaca cttttacact gttggtggga
     6181 ctgtaaacta gttcaaccat tgtggaagtc agtgtggcga ttcctcaggg atctagaact
     6241 agaaatacca tttgacccag ccatcccatt actgggtata tacccaaatg agtataaatc
     6301 atgctgctat aaagacacat gcacacgtat gtttattgcg gcactattca caatagcaaa
     6361 gacttggaac caacccaaat gtccaacaat gatagactgg attaagaaaa tgtggcacat
     6421 atacaccatg gaatactatg cagccataaa aaatgatgag ttcatatcct ttgtagggac
     6481 atggatgaaa ttggaaacca tcattctcag taaactatcg caagaacaaa aaaccaaaca
     6541 ccgcatattc tcactcatag gtgggaatta gacaatgaga tcacatggac acaggaaggg
     6601 gaatatcaca ctctggggac tgtggtgggg tcgggggagg ggggagggat agcattggga
     6661 gatataccta atgctagatg acacattagt gggtgcagcg caccagcatg gcacatgtat
     6721 acatatgtaa ctaacctgca caatgtgcac atgtacccta aaacttagag tataatagat
     6781 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     6841 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     6901 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     6961 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     7021 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7081 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7141 cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgat cgactctaga ggatcgatcc
     7201 ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc
     7261 atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca
     7321 cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat
     7381 gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac
     7441 tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg
     7501 ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga
     7561 ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata
     7621 aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat
     7681 ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt
     7741 agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt
     7801 tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag
     7861 tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc
     7921 tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg
     7981 ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg
     8041 tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt
     8101 gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc
     8161 gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt
     8221 gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca
     8281 tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc
     8341 cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata
     8401 aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc
     8461 ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt
     8521 aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg
     8581 gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag
     8641 gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca
     8701 cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata
     8761 tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     8821 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     8881 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     8941 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga
     9001 ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg
     9061 gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     9121 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     9181 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     9241 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     9301 ggtagcatat gctatcctca tgcatataca gtcagcatat gatacccagt agtagagtgg
     9361 gagtgctatc ctttgcatat gccgccacct cccaaggggg cgtgaatttt cgctgcttgt
     9421 ccttttcctg ctggttgctc ccattcttag gtgaatttaa ggaggccagg ctaaagccgt
     9481 cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg agaaggtgtt
     9541 gagcgcggag ctgagtgacg tgacaacatg ggtatgccca attgccccat gttgggagga
     9601 cgaaaatggt gacaagacag atggccagaa atacaccaac agcacgcatg atgtctactg
     9661 gggatttatt ctttagtgcg ggggaataca cggcttttaa tacgattgag ggcgtctcct
     9721 aacaagttac atcactcctg cccttcctca ccctcatctc catcacctcc ttcatctccg
     9781 tcatctccgt catcaccctc cgcggcagcc ccttccacca taggtggaaa ccagggaggc
     9841 aaatctactc catcgtcaaa gctgcacaca gtcaccctga tattgcaggt aggagcgggc
     9901 tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat atgagtttgt
     9961 aaaaagacca tgaaataaca gacaatggac tcccttagcg ggccaggttg tgggccgggt
    10021 ccaggggcca ttccaaaggg gagacgactc aatggtgtaa gacgacattg tggaatagca
    10081 agggcagttc ctcgccttag gttgtaaagg gaggtcttac tacctccata tacgaacaca
    10141 ccggcgaccc aagttccttc gtcggtagtc ctttctacgt gactcctagc caggagagct
    10201 cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc acgatgcttt
    10261 ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct gaccccgggg tccagtgctt
    10321 gggccttctc ctgggtcatc tgcggggccc tgctctatcg ctcccggggg cacgtcaggc
    10381 tcaccatctg ggccaccttc ttggtggtat tcaaaataat cggcttcccc tacagggtgg
    10441 aaaaatggcc ttctacctgg agggggcctg cgcggtggag acccggatga tgatgactga
    10501 ctactgggac tcctgggcct cttttctcca cgtccacgac ctctccccct ggctctttca
    10561 cgacttcccc ccctggctct ttcacgtcct ctaccccggc ggcctccact acctcctcga
    10621 ccccggcctc cactacctcc tcgaccccgg cctccactgc ctcctcgacc ccggcctcca
    10681 cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
    10741 cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc
    10801 cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
    10861 gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct
    10921 cctgcccctc ctscycctcc tcctcctcct cctccccctc cyccccctcc tccycctccc
    10981 ccycctccyc ctccycctcc tcyycctcct cctcctcckc ctcctcctcc tcctcctgcy
    11041 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tcctcctcct
    11101 ccycctcccc ctcctccccc tccccccccy ccccctcctc cycctcytcc tccycctccy
    11161 ccysctcctc cccctcctcc tsctcctccy cctcctccyc ctcctcctcc tcctcctcct
    11221 cctcctcctc ctcctccycc tcctcctcct cctcctcctc ctccccctcc tcctcctscy
    11281 cctcctgccc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgct
    11341 cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc accgtgggtc
    11401 cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc tctatgtctt
    11461 ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt
    11521 ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc gccggagcct
    11581 tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt acctggcccc
    11641 tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga cgacgctcag
    11701 tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct catccccttc
    11761 atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac catcctcgtc
    11821 ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc
    11881 ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg gtagggatgg
    11941 ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg ctactggggc
    12001 aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata agctgtcaaa
    12061 catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
    12121 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
    12181 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    12241 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    12301 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    12361 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    12421 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    12481 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
    12541 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    12601 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    12661 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    12721 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    12781 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
    12841 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    12901 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    12961 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    13021 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    13081 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    13141 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    13201 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    13261 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    13321 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    13381 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    13441 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    13501 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    13561 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    13621 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    13681 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    13741 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    13801 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    13861 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    13921 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    13981 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    14041 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
    14101 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
    14161 gatgccgcat agttaagcca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
    14221 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
    14281 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    14341 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
    14401 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
    14461 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
    14521 aagcttgcat gcctgcaggt cggccgccac gaccggtgcc gccaccatcc cctgacccac
    14581 gcccctgacc cctcacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc
    14641 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
    14701 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
    14761 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
    14821 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
    14881 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
    14941 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
    15001 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
    15061 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
    15121 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
    15181 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
    15241 ccgaaaggag cgcacgaccc catggctccg accgaagcca cccggggcgg ccccgccgac
    15301 cccgcacccg cccccgaggc ccaccgactc tagaggatca taatcagcca taccacattt
    15361 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    15421 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    15481 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    15541 tccaaactca tcaatgtatc ttatcatgtc tggatcactc gccgatagtg gaaaccgacg
    15601 ccccagcact cgtccggatc gggagatggg ggaggctaac tgaaacacgg aaggagacaa
    15661 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
    15721 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
    15781 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
    15841 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
    15901 actggccccg tgggttaggg acggggtccc ccatggggaa tggtttatgg ttcgtggggg
    15961 ttattatttt gggcgttgcg tggggtcagg tccacgactg gactgagcag acagacccat
    16021 ggtttttgga tggcctgggc atggaccgca tgtactggcg cgacacgaac accgggcgtc
    16081 tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg cggatttctg gcgtgccaag
    16141 ctagtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
    16201 cattgctgca ggcgcagaac tggtaggtat ggaagatctc tagaagctgg gtaccagctg
    16261 ctagcaagct tgctagcggc cgaggcgtat cacgaggccc tttcgtcttc actcgagttt
    16321 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
    16381 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
    16441 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
    16501 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
    16561 tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaacc
//
