LOCUS       sequence-42919-e        3000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CmCherry-ABCG2a
FEATURES             Location/Qualifiers
     source          1..3000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2163..2873
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2308..2326)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2576..2595
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2948..2967)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctagtggcgc gccaatggag ggagaagagc atgagcagga cacgcacttc ggggcgacgg
       61 aggaaacacc gacgactgtc gcaccgagca gggacccaga gaacgaccag cccgccgtac
      121 tggaagtcaa ccacaatgat accggccctg gggatatgaa gatcaatatg gatccggtac
      181 tctcagaccc agagaagcac aagctaggat atggcagcgt gacgaatgga cactcgagtc
      241 acacaaaggt ccatccatcc tttgccagga gtacctccat tgatggatcg gtagtgtcct
      301 ttcatgccat cacctacacg gtcaagacca aagagaatcg caagaaagtg accaagacta
      361 tcctcacaga cgtcaatgga ttattcaagc ctggaatgaa cgccattctt ggacctacag
      421 gaagcggcaa aacatcattg ttggatgtgt tggcagctcg gaaagaccca gacggactgt
      481 cgggtcaagt cctcatagat ggcgccccac aaccaaagaa cttcaaatgc gtctccggat
      541 atgttgtcca ggatgatgtc gtcatgggaa cgctcaccat cagagagaat cttcagttct
      601 ctgctgctct ccgtctcccc aagacggtcg gtaagaagga gagagaggat agggtggatg
      661 atatcctagc tgagctaggt ctttcacacg tcggtgatag taaggttgga acagagttca
      721 tccgaggcgt gtcaggagga gagagaaaga gaaccaatgt tgggatggag ctcatcacaa
      781 aacccagtgt tctgttctta gacgaaccaa cgacaggatt ggatgcaagt actgctaatg
      841 ctgtcatgca tctgttagcc agtctgtcca agagagggcg caccatcatc ttctccatcc
      901 atcagcctcg ctactccatc ttccgtctct ttgacaagat gcacctgttg ggacaagggc
      961 ggacgatcta ccatggcccc gcccaagaag ctttggagta tttttcatct attggttttg
     1021 agtgtgaaga acacaacaat ccacctgact tcttcttgga tgtcatcctg ggacagtctg
     1081 atctgaagtc atcggagaaa gatcaagaag atgtagagag tggtgtggca gccatagaga
     1141 atggagagaa gagcaaagtg atcctagcag attgctacga gaagtctcac tacaacaaga
     1201 cgatgaaaga ggctacagag agcatctaca cccagtaccg caacgacgaa gaaccgagca
     1261 tcgtttccat caactaccca acatccttct tttcacagtt gtaccatgtt tcccatcgag
     1321 cagtactgaa catcctcaga aatccattcc tgaccgtaat ccagaacgcg actgtcatat
     1381 tattctctgt tgttatcggc ggcatctact ttcaactcga cacctccatc tcgtcaggat
     1441 accagaacag aattggtgcc ttcttcttcc tcatcatgca aatggtattt ggcaacttga
     1501 gtgctgttga gcttttcatc agggaaagag ttatatttat tcatgaatcg gcgagtggtt
     1561 tctaccgagt ctcggtgtac ttcgttgcga aggtcttttg tgatctactt ccgctaagag
     1621 tgataccaac aatcctttat gtcgttgtca cgtattggat gattggtctt caagctgatg
     1681 caacaaagtt cttcttgtac ttcttgactc tccttctcgt cacatttgtg tccagcgccc
     1741 tcgcctttgc catcagttcc agtgtgagca tcgcaggcat agctactctg ctcattgcca
     1801 tgtgctatgt gctcatgatg gtatttggag gactgttggt gaacatcagc tcattgcctg
     1861 tctggcttca atggcttcag tatctcagca tcttcagatt tggtctaaat gctctgctaa
     1921 ttaacgagat ggttggtttg gacttctgtg agactgtagg aaatgtcacc ctacgatgta
     1981 tccccggtac cgactacctc gatcagcaag gaatcgacta cagcgactgg ggcctttggc
     2041 agaatgagat ggccctagga atcatgaccg tcgcccttct agccatcgct tacattcagc
     2101 tcaggaggat acctaagctc aaattaatta aaggccggcc agcgatcgcc ggacatcgat
     2161 tgatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     2221 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     2281 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     2341 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     2401 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     2461 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     2521 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     2581 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     2641 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     2701 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     2761 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     2821 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taagaattca
     2881 aggcctctcg agcctctaga actatagtga gtcgtattac gtagatccag acatgataag
     2941 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
//
