LOCUS       sequence-429197-        8042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human CREB3L1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   De Marchi F, Okuda M, Morishita S, Imai M, Baba T, Horino M, Mori Y,
            Furuya C, Ogata S, Yang Y, Ando J, Ando M, Araki M, Komatsu N
  TITLE     Clinical and biological relevance of CREB3L1 in Philadelphia 
            chromosome-negative myeloproliferative neoplasms.
  JOURNAL   Leuk Res. 2022 Aug;119:106883. doi: 10.1016/j.leukres.2022.106883. 
            Epub 2022 May 31.
  PUBMED    35689957
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Leuk Res.";
            date: "2022-08"; volume: "119:106883. doi"; pages: " 
            10.1016/j.leukres.2022.106883. Epub 2022 May 31"
COMMENT     Sequence Label: pMSCV-IRES-GFP/CREB3L1
FEATURES             Location/Qualifiers
     source          1..8042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(572..591)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(719..741)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(935..954)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1054..1076
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1114..1132)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1200..1304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1305..1373,1374..2165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1523..1542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2336..2924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2825..2844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3078..3095
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3212..3233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3248..3253,3254..3271,3272..3278)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3286..3302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             3464..3978
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(4139..4849)
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    4856..5405
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5015..5034)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5244..5261
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     regulatory      6537..6546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6993..7002
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7010..7426)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(7026..7042)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7058..7080)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    7493..7834
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             join(7898..8042,1..372)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 gccgagtgag gggttgtggg ctcttttatt gagctcgggg agcagaagcg cgcgaacaga
       61 agcgagaagc gaactgattg gttagttcaa ataaggcaca gggtcatttc aggtccttgg
      121 ggcaccctgg aaacatctga tggttctcta gaaactgctg agggcgggac cgcatctggg
      181 gaccatctgt tcttggccct gagccggggc aggaactgct taccacagat atcctgtttg
      241 gcccatattc tgctgtctct ctgttcctaa ccttgatctg aacttctcta ttctcagtta
      301 tgtattttcc atgccttgca aaatggcgtt acttaagcta gcttgccaaa cctacaggtg
      361 gggtctttca ttcccccctt tttctggaga ctaaataaaa tcttttattt tatctatggc
      421 tcgtactcta taggcttcag ctggtgatat tgttgagtca aaactagagc ctggaccact
      481 gatatcctgt ctttaacaaa ttggactaat cgcctctacg ccggacgcat cgtggccggc
      541 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
      601 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
      661 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcgccgtcg
      721 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      781 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      841 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      901 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      961 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     1021 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     1081 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     1141 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1201 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1261 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1321 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1381 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1441 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1501 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1561 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1621 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1681 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1741 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1801 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1861 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1921 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1981 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2041 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2101 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2161 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2221 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2281 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2341 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2401 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2461 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2521 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2581 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2641 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2701 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2761 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2821 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2881 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2941 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3001 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3061 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3121 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3181 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     3241 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     3301 aatttcacaa ttctccccag ctctttctgc gctgtccata atgaactatt tcaggagtat
     3361 tggagtatgt ggattcaaaa cagtggctaa ttgagtcagg attacttaat attcgactcc
     3421 taccctcaga atgttgttct ccaacttcaa gaaactgtta cccatgaaag acccccgctg
     3481 acgggtagtc aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat
     3541 gcaactgcaa gagggtttat tggatacacg ggtacccggg cgacgcagtc tatcggagga
     3601 ctggcgcgcc gagtgagggg ttgtgggctc ttttattgag ctcggggagc agaagcgcgc
     3661 gaacagaagc gagaagcgaa ctgattggtt agttcaaata aggcacaggg tcatttcagg
     3721 tccttggggc accctggaaa catctgatgg ttctctagaa actgctgagg gcgggaccgc
     3781 atctggggac catctgttct tggccctgag ccggggcagg aactgcttac cacagatatc
     3841 ctgtttggcc catattctgc tgtctctctg ttcctaacct tgatctgaac ttctctattc
     3901 tcagttatgt attttccatg ccttgcaaaa tggcgttact taagctagct tgccaaacct
     3961 acaggtgggg tctttcattc cccccttttt ctggagacta aataaaatct tttattttat
     4021 cgatatcagc gctttaaatt tgcgcatgct agctatagtt ctagaggtac cggttgttaa
     4081 cgttagccgg ctacctgaag cttgcatgcc tgcaggtcga ctctagagtc gcggccgctt
     4141 acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt
     4201 ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgtcg acgatgtagg
     4261 cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctcg gaggtgtagt
     4321 ggccgccgtc cttcagcttc agcctcatct tgatcttgcc cttcagggca ccgtcctcgg
     4381 ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatc acggggccgt
     4441 cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg
     4501 aggagtcctg ggtcacggtc accacgccgc cgtcctcgta gttcatcacg cgctcccact
     4561 tgaagccctc ggggaaggac agcttgaagt agtcggggat gtcggcgggg tgcttcacgt
     4621 aggccttgga gccgtaggtg aaatgagggg acaggatgtc ccaggcgaag ggcagggggc
     4681 cacccttggt caccttcagc ttagcggtct gaaagccctc gtaggggcgg ccctcgccct
     4741 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgcgcacc ttgaagcgca
     4801 tgaactcctt gatgatggcc atgttattct cctcgccctt gctcaccatt catggtatta
     4861 tcgtgttttt caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa
     4921 cctcgactaa acacatgtaa agcatgtgca ccgaggcccc agatcagatc ccatacaatg
     4981 gggtaccttc tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac
     5041 tctttccaca actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt
     5101 atcttataca cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg
     5161 cctgcaaagg gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc
     5221 cttcacgaca ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat
     5281 gctcgtcaag aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat
     5341 ggtggaaaat aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt
     5401 aacgttaggg ggggggcgga attgatcccg ctcgagctag gagagtttga tggtggtgtt
     5461 ggggcccaga tccctgtcgt ggaaccactc cttggagtgg gagaagtcgg ggctggtgcc
     5521 gtttccaccg tctttaggcc aggcctcact caggtacttg gtggtctcgt gggtgctgtc
     5581 caggtgatca tgctgcaggt ggtcccgggg ccgctgctct gccggccccc cggggttgat
     5641 ttcccagcca tctgggggct ccatgggcag cagggtgctg cggccatctt cccataagcc
     5701 tgccccgtca tcgtagaata ggaggcttcg ggagggcatc tggctggccg tgtagacgcc
     5761 gtctgcggcc agggggtctt ccttcacagt ctgggagccg gaggagaact cgggaaggca
     5821 gggcacgagg gagcccagca ccagaacaaa gcacaaggct gccaccatga ggcaggtccc
     5881 agtctgggtg gcggccatct tgtaaggtct ggagatcttg ttggtgacca gagtctggag
     5941 tttctgcagc tgctggagca gggtcctgtt ggcattctcc agggtctcca ccttcttcca
     6001 cagttcattg ttctcagatg taaatgtctc caccttcttt tctagacact ccacatactc
     6061 cttcttctta cgacggctct cctgggctga gatcttgttc ttgattttcc tccggactct
     6121 cttcaaggcc ttctcctcgg ctttggtgag ggggagtttt gtggggatgg ggtagccctc
     6181 agcaatcagg gtccgcttct cctcctctgt caggagcagt ggccctgatg tcccctgtaa
     6241 tttgtgaggg gcagtgagga gtggggaggt ggagatggcc gtggaggagc gcgccatggg
     6301 cctgacaggg ctggaggggg gcagagagcg gggactctgg gagccgtcgc tgtcactgcc
     6361 atggctgctg gggggcgtcg gaggcatctg caccaggtcc tccggtgtca ctttgaggaa
     6421 ctggttcacc tccagaggct ctgctttgat cactggcagc tgagtcatct ctcccggggc
     6481 ctggtggggg atgggcagcc tggacaaggg gctgaggccc agcagcgggg tggtggccat
     6541 ggcggccgcg gcagccatgg ccgagggggc agccagaggg tccacgggca gctccgggct
     6601 ctgctcctgc ttcaccatga tggagcacag tttgtgtccc agcgcccatg ctccatgctc
     6661 tgcatcttgg gtggtgtcct ccatcttgat gggcacaagg gggctctggg gcgctgagtc
     6721 gccgctcagg gagtagctgt gctccgcctg gatgcctggc gtaggggagt ccagttccat
     6781 gtccaataga gggctcttct catccagcac agggtcatca aagaagctgc tgaacaggtc
     6841 attggagaag tcctccatgt tctccgtaaa gtggtccagg tgctcaggaa agtgcgcatt
     6901 gttgaggaag tccgactcgt tcagatcccc caagtccagg aagctggatc cggggaacag
     6961 cctgtcggcc gggaagggtt ccaagacggc gtccatggtg gcgaattccg gcgcctagag
     7021 aaggagtgag ggctggataa agggaggatc gaggcggggt cgaacgagga ggttcaaggg
     7081 ggagagacgg ggcggatgga ggaagaggag gcggaggctt agggtgtaca aagggcttga
     7141 cccagggagg ggggtcaaaa gccaaggctt cccaggtcac gatgtagggg acctggtctg
     7201 ggtgtccatg cgggccaggt gaaaagacct tgatcttaac ctgggtgatg aggtctcggt
     7261 taaaggtgcc gtctcgcggc catccgacgt taaaggttgg ccattctgca gagcagaagg
     7321 taacccaacg tctcttcttg acatctaccg actggttgtg agcgatccgc tcgacatctt
     7381 tccagtgacc taaggtcaaa cttaagggag tggtaacagt ctggccctaa ttttcagaca
     7441 aatacagaaa cacagtcaga cagagacaac acagaacgat gctgcagcgc tgcagcagac
     7501 aagacgcgcg gcttcggttc caaaccgaaa gcaaaaattc agacggaggc gggaactgtt
     7561 ttaggttctc gtctcctacc agaaccacat atcctgacgg ggtcggattc cacatcgact
     7621 cccttcctca ggtcgggcca caaaaacggc ccccaaagtc cctgggacgt ctcccagggt
     7681 tgcggccggg tgttcagaac tcgtcagttc caccacgggt ccgccagata cagagctagt
     7741 tagctaacta gtacagacgc aggcgcaaac attagatgcc ggcacaaaca cgcacaaaga
     7801 cagagacaga cacgaaacga ccgctggcca gcttacctcc cggcgggggg gtcggtggtc
     7861 cctgggcagg ggtctccaaa tctcggtgga acctccaaat gaaagacccc cgaggtgggc
     7921 agtcaatcaa tctgaggaga ccctcccaag gatcagcgag tccacgattc ggatgcaaac
     7981 agcaagaggc tttattggga atacgggtac ccgggcgacg cagtctatcg gaggactggc
     8041 gc
//
