LOCUS       sequence-42920-e        3009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CCerulean-ABCG2a
FEATURES             Location/Qualifiers
     source          1..3009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(2163..2165,2166..2168,2169..2882)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2208..2229)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2469..2488)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2816..2837
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2957..2976)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctagtggcgc gccaatggag ggagaagagc atgagcagga cacgcacttc ggggcgacgg
       61 aggaaacacc gacgactgtc gcaccgagca gggacccaga gaacgaccag cccgccgtac
      121 tggaagtcaa ccacaatgat accggccctg gggatatgaa gatcaatatg gatccggtac
      181 tctcagaccc agagaagcac aagctaggat atggcagcgt gacgaatgga cactcgagtc
      241 acacaaaggt ccatccatcc tttgccagga gtacctccat tgatggatcg gtagtgtcct
      301 ttcatgccat cacctacacg gtcaagacca aagagaatcg caagaaagtg accaagacta
      361 tcctcacaga cgtcaatgga ttattcaagc ctggaatgaa cgccattctt ggacctacag
      421 gaagcggcaa aacatcattg ttggatgtgt tggcagctcg gaaagaccca gacggactgt
      481 cgggtcaagt cctcatagat ggcgccccac aaccaaagaa cttcaaatgc gtctccggat
      541 atgttgtcca ggatgatgtc gtcatgggaa cgctcaccat cagagagaat cttcagttct
      601 ctgctgctct ccgtctcccc aagacggtcg gtaagaagga gagagaggat agggtggatg
      661 atatcctagc tgagctaggt ctttcacacg tcggtgatag taaggttgga acagagttca
      721 tccgaggcgt gtcaggagga gagagaaaga gaaccaatgt tgggatggag ctcatcacaa
      781 aacccagtgt tctgttctta gacgaaccaa cgacaggatt ggatgcaagt actgctaatg
      841 ctgtcatgca tctgttagcc agtctgtcca agagagggcg caccatcatc ttctccatcc
      901 atcagcctcg ctactccatc ttccgtctct ttgacaagat gcacctgttg ggacaagggc
      961 ggacgatcta ccatggcccc gcccaagaag ctttggagta tttttcatct attggttttg
     1021 agtgtgaaga acacaacaat ccacctgact tcttcttgga tgtcatcctg ggacagtctg
     1081 atctgaagtc atcggagaaa gatcaagaag atgtagagag tggtgtggca gccatagaga
     1141 atggagagaa gagcaaagtg atcctagcag attgctacga gaagtctcac tacaacaaga
     1201 cgatgaaaga ggctacagag agcatctaca cccagtaccg caacgacgaa gaaccgagca
     1261 tcgtttccat caactaccca acatccttct tttcacagtt gtaccatgtt tcccatcgag
     1321 cagtactgaa catcctcaga aatccattcc tgaccgtaat ccagaacgcg actgtcatat
     1381 tattctctgt tgttatcggc ggcatctact ttcaactcga cacctccatc tcgtcaggat
     1441 accagaacag aattggtgcc ttcttcttcc tcatcatgca aatggtattt ggcaacttga
     1501 gtgctgttga gcttttcatc agggaaagag ttatatttat tcatgaatcg gcgagtggtt
     1561 tctaccgagt ctcggtgtac ttcgttgcga aggtcttttg tgatctactt ccgctaagag
     1621 tgataccaac aatcctttat gtcgttgtca cgtattggat gattggtctt caagctgatg
     1681 caacaaagtt cttcttgtac ttcttgactc tccttctcgt cacatttgtg tccagcgccc
     1741 tcgcctttgc catcagttcc agtgtgagca tcgcaggcat agctactctg ctcattgcca
     1801 tgtgctatgt gctcatgatg gtatttggag gactgttggt gaacatcagc tcattgcctg
     1861 tctggcttca atggcttcag tatctcagca tcttcagatt tggtctaaat gctctgctaa
     1921 ttaacgagat ggttggtttg gacttctgtg agactgtagg aaatgtcacc ctacgatgta
     1981 tccccggtac cgactacctc gatcagcaag gaatcgacta cagcgactgg ggcctttggc
     2041 agaatgagat ggccctagga atcatgaccg tcgcccttct agccatcgct tacattcagc
     2101 tcaggaggat acctaagctc aaattaatta aaggccggcc agcgatcgcc ggacatcgat
     2161 tgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2221 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2281 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2341 ccctcgtgac caccctgacc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga
     2401 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2461 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2521 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2581 acaagctgga gtacaacgcc atcagcgaca acgtctatat caccgccgac aagcagaaga
     2641 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2701 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2761 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     2821 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     2881 aagaattcaa ggcctctcga gcctctagaa ctatagtgag tcgtattacg tagatccaga
     2941 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     3001 ctttatttg
//
