LOCUS       sequence-429200-        7730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human CALR del52.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   De Marchi F, Okuda M, Morishita S, Imai M, Baba T, Horino M, Mori Y,
            Furuya C, Ogata S, Yang Y, Ando J, Ando M, Araki M, Komatsu N
  TITLE     Clinical and biological relevance of CREB3L1 in Philadelphia 
            chromosome-negative myeloproliferative neoplasms.
  JOURNAL   Leuk Res. 2022 Aug;119:106883. doi: 10.1016/j.leukres.2022.106883. 
            Epub 2022 May 31.
  PUBMED    35689957
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Leuk Res.";
            date: "2022-08"; volume: "119:106883. doi"; pages: " 
            10.1016/j.leukres.2022.106883. Epub 2022 May 31"
COMMENT     Sequence Label: pMSCV-IRES-mOrange/CALR del52
FEATURES             Location/Qualifiers
     source          1..7730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    169..510
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             577..993
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     923..945
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     961..977
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    2286..2835
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2430..2447)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2657..2676
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2842..3552
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     LTR             3713..4227
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    4389..4405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4413..4419,4420..4437,4438..4443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4458..4479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4596..4613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4767..5355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4847..4866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5526..6317,6318..6386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6149..6168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6387..6491)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6559..6577
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6615..6637)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6737..6756
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6950..6972
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7100..7119
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(7319..7730,1..105)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 attcccaata aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag
       61 ggtctcctca gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga
      121 gatttggaga cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc
      181 ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc
      241 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      301 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
      361 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
      421 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
      481 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
      541 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
      601 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
      661 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
      721 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
      781 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
      841 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
      901 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
      961 ctttatccag ccctcactcc ttctctaggc gccggaattc gccaccatgc tgctatccgt
     1021 gccgctgctg ctcggcctcc tcggcctggc cgtcgccgag cctgccgtct acttcaagga
     1081 gcagtttctg gacggagacg ggtggacttc ccgctggatc gaatccaaac acaagtcaga
     1141 ttttggcaaa ttcgttctca gttccggcaa gttctacggt gacgaggaga aagataaagg
     1201 tttgcagaca agccaggatg cacgctttta tgctctgtcg gccagtttcg agcctttcag
     1261 caacaaaggc cagacgctgg tggtgcagtt cacggtgaaa catgagcaga acatcgactg
     1321 tgggggcggc tatgtgaagc tgtttcctaa tagtttggac cagacagaca tgcacggaga
     1381 ctcagaatac aacatcatgt ttggtcccga catctgtggc cctggcacca agaaggttca
     1441 tgtcatcttc aactacaagg gcaagaacgt gctgatcaac aaggacatcc gttgcaagga
     1501 tgatgagttt acacacctgt acacactgat tgtgcggcca gacaacacct atgaggtgaa
     1561 gattgacaac agccaggtgg agtccggctc cttggaagac gattgggact tcctgccacc
     1621 caagaagata aaggatcctg atgcttcaaa accggaagac tgggatgagc gggccaagat
     1681 cgatgatccc acagactcca agcctgagga ctgggacaag cccgagcata tccctgaccc
     1741 tgatgctaag aagcccgagg actgggatga agagatggac ggagagtggg aacccccagt
     1801 gattcagaac cctgagtaca agggtgagtg gaagccccgg cagatcgaca acccagatta
     1861 caagggcact tggatccacc cagaaattga caaccccgag tattctcccg atcccagtat
     1921 ctatgcctat gataactttg gcgtgctggg cctggacctc tggcaggtca agtctggcac
     1981 catctttgac aacttcctca tcaccaacga tgaggcatac gctgaggagt ttggcaacga
     2041 gacgtggggc gtaacaaagg cagcagagaa acaaatgaag gacaaacagg acgaggagca
     2101 gaggacaagg aggatgatga ggacaaagat gaggatgagg aggatgagga ggacaaggag
     2161 gaagatgagg aggaagatgt ccccggccag gccaaggacg agctgtagag aggcctgcct
     2221 ccagggctgg actgaggcct gagaattcgt taacctcgag cgggatcaat tccgcccccc
     2281 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     2341 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     2401 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     2461 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     2521 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     2581 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     2641 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     2701 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     2761 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     2821 aaaaacacga taataccatg aatggtgagc aagggcgagg agaataacat ggccatcatc
     2881 aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag
     2941 atcgagggcg agggcgaggg ccgcccctac gagggctttc agaccgctaa gctgaaggtg
     3001 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcattt cacctacggc
     3061 tccaaggcct acgtgaagca ccccgccgac atccccgact acttcaagct gtccttcccc
     3121 gagggcttca agtgggagcg cgtgatgaac tacgaggacg gcggcgtggt gaccgtgacc
     3181 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     3241 ttcccctccg acggccccgt gatgcagaag aagaccatgg gctgggaggc ctcctccgag
     3301 cggatgtacc ccgaggacgg tgccctgaag ggcaagatca agatgaggct gaagctgaag
     3361 gacggcggcc actacacctc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
     3421 ctgcccggcg cctacatcgt cgacatcaag ttggacatca cctcccacaa cgaggactac
     3481 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     3541 ctgtacaagt aagcggccgc gactctagag tcgacctgca ggcatgcaag cttcaggtag
     3601 ccggctaacg ttaacaaccg gtacctctag aactatagct agcatgcgca aatttaaagc
     3661 gctgatatcg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     3721 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaat
     3781 acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca gcagaatatg
     3841 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     3901 gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     3961 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     4021 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     4081 ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgtatcca ataaaccctc
     4141 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
     4201 gactacccgt cagcgggggt ctttcatggg taacagtttc ttgaagttgg agaacaacat
     4261 tctgagggta ggagtcgaat attaagtaat cctgactcaa ttagccactg ttttgaatcc
     4321 acatactcca atactcctga aatagttcat tatggacagc gcagaaagag ctggggagaa
     4381 ttgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4441 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4501 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4561 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4621 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4681 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4741 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4801 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4861 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4921 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4981 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5041 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5101 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5161 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5221 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5281 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5341 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5401 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5461 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5521 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5581 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5641 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5701 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5761 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5821 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5881 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5941 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6001 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6061 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6121 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6181 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6241 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6301 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6361 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6421 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6481 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     6541 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     6601 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6661 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6721 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     6781 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     6841 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     6901 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     6961 cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     7021 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     7081 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     7141 cgccggtgat gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag
     7201 acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta
     7261 tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     7321 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     7381 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
     7441 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     7501 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
     7561 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     7621 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     7681 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt
//
