LOCUS       sequence-429204-        6482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   De Marchi F, Okuda M, Morishita S, Imai M, Baba T, Horino M, Mori Y,
            Furuya C, Ogata S, Yang Y, Ando J, Ando M, Araki M, Komatsu N
  TITLE     Clinical and biological relevance of CREB3L1 in Philadelphia 
            chromosome-negative myeloproliferative neoplasms.
  JOURNAL   Leuk Res. 2022 Aug;119:106883. doi: 10.1016/j.leukres.2022.106883. 
            Epub 2022 May 31.
  PUBMED    35689957
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Leuk Res.";
            date: "2022-08"; volume: "119:106883. doi"; pages: " 
            10.1016/j.leukres.2022.106883. Epub 2022 May 31"
COMMENT     Sequence Label: pMSCV-IRES-mOrange
FEATURES             Location/Qualifiers
     source          1..6482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    169..510
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             577..993
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     923..945
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     961..977
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1038..1587
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1182..1199)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1409..1428
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1594..2304
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     LTR             2465..2979
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    3141..3157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3165..3171,3172..3189,3190..3195))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3210..3231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3348..3365)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3519..4107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3599..3618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4278..5069,5070..5138))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4901..4920
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5139..5243)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5311..5329
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5367..5389)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5489..5508
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5702..5724
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5852..5871
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(6071..6482,1..105)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 attcccaata aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag
       61 ggtctcctca gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga
      121 gatttggaga cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc
      181 ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc
      241 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      301 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
      361 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
      421 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
      481 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
      541 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
      601 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
      661 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
      721 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
      781 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
      841 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
      901 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
      961 ctttatccag ccctcactcc ttctctaggc gccggaattc gttaacctcg agcgggatca
     1021 attccgcccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     1081 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     1141 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     1201 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     1261 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     1321 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     1381 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     1441 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     1501 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     1561 ggttttcctt tgaaaaacac gataatacca tgaatggtga gcaagggcga ggagaataac
     1621 atggccatca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
     1681 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggctt tcagaccgct
     1741 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcat
     1801 ttcacctacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttcaag
     1861 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga actacgagga cggcggcgtg
     1921 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     1981 cgcggcacca acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag
     2041 gcctcctccg agcggatgta ccccgaggac ggtgccctga agggcaagat caagatgagg
     2101 ctgaagctga aggacggcgg ccactacacc tccgaggtca agaccaccta caaggccaag
     2161 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cacctcccac
     2221 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2281 ggcatggacg agctgtacaa gtaagcggcc gcgactctag agtcgacctg caggcatgca
     2341 agcttcaggt agccggctaa cgttaacaac cggtacctct agaactatag ctagcatgcg
     2401 caaatttaaa gcgctgatat cgataaaata aaagatttta tttagtctcc agaaaaaggg
     2461 gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa
     2521 ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg aacagagaga
     2581 cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     2641 caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag aaccatcaga
     2701 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
     2761 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
     2821 aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta cccgtgtatc
     2881 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
     2941 ctctgagtga ttgactaccc gtcagcgggg gtctttcatg ggtaacagtt tcttgaagtt
     3001 ggagaacaac attctgaggg taggagtcga atattaagta atcctgactc aattagccac
     3061 tgttttgaat ccacatactc caatactcct gaaatagttc attatggaca gcgcagaaag
     3121 agctggggag aattgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3181 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3241 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3301 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3361 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3421 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3481 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3541 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3601 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3661 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3721 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3781 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3841 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3901 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3961 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4021 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4081 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4141 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4201 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4261 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4321 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4381 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4441 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4501 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4561 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4621 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4681 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4741 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4801 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4861 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4921 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4981 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5041 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5101 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5161 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5221 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     5281 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     5341 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     5401 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     5461 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     5521 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     5581 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5641 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     5701 tcccagtcac gacgttgtaa aacgacggcg caaggaatgg tgcatgcaag gagatggcgc
     5761 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     5821 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     5881 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc
     5941 aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc
     6001 agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt agtctccaga
     6061 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     6121 tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac
     6181 agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     6241 tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac
     6301 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     6361 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     6421 agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc
     6481 gt
//
