LOCUS       sequence-42921-e        1256 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NeGFP
FEATURES             Location/Qualifiers
     source          1..1256
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        213..231
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     213..230
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             278..991
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(308..336)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     931..951
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    1084..1218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1108..1127)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1162..1181
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cattggcagt actcccattg acgtcaatgg cggtaaatgg cccgcgatgg ctgccaagta
       61 catcccccat tgacgtcaat ggggaggggc aatgacgcaa atgggcgttc cattgacgta
      121 aatgggcggt aggcgtgcct aatgggaggt ctatataagc aatgctcgtt tagggaaccg
      181 ccattctgcc tggggacgtc ggagcaagct tgatttaggt gacactatag aatacaagct
      241 acttgttctt tttgcaggat ccactagtca tcgattgatg agtaaaggag aagaactttt
      301 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      361 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
      421 cactactgga aaactacctg ttccatggcc aacacttgtc actactctca cttatggtgt
      481 tcaatgcttt tcaagatacc cagatcatat gaaacggcat gactttttca agagtgccat
      541 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
      601 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
      661 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
      721 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
      781 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
      841 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
      901 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
      961 gattacacat ggcatggatg aactatacaa agaattcgga gccggcgcgc cattaattaa
     1021 aggccggcca gcgatcgcca aggcctctcg agcctctaga actatagtga gtcgtattac
     1081 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     1141 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     1201 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttca
//
