LOCUS       sequence-42922-e        5710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NCerulean-ABCB1a
FEATURES             Location/Qualifiers
     source          1..5710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(15..17,18..20,21..731)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(60..81)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(321..340)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     668..689
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4795..4929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4819..4838)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4873..4892
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5042..5062)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5042..5060)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5081..5097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5081..5097)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5094..5116)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5105..5121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5129..5135,5136..5153,5154..5159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5174..5195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5312..5329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5563..5582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagtcatcg attgatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
       61 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      121 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      181 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc gcccgctacc
      241 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      301 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      361 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      421 acatcctggg gcacaagctg gagtacaacg ccatcagcga caacgtctat atcaccgccg
      481 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca
      541 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      601 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
      661 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      721 agctgtacaa ggaattcgga gccggcgcgc cattaattaa aggccggcca atggtagagg
      781 gtgacccacc tgctgtgggt ttctccaatg gcaatggaac agtgcagatg aatggaaatg
      841 gagctagctt tgatggacca gccgtgatcg atgtgtcgcc tatacatgag gacgctcctt
      901 ctgctaagca tgcgagagac tctcctcccc ctgcctacag tgatgtcagt ggaacgaagg
      961 aacagggaaa tggaggatct gtggaaggag agggtggaga gaaggaagac aagaaagaga
     1021 caaaagtagt gggcacgttt gaagtgtttc gctttgcaac tggtctagac gtcgtgttca
     1081 ttctgctagc tcttgtgatc tctctctgcc atggtgtggc cttgcctgcc gttcttcttc
     1141 tatttggaga agtcacagat tctttcatca ccacagcctc cgtgaacgtg acagacaatt
     1201 tggcagcctt tgaggaatcg gtggacagta ttataacttt ctccatctac tattcctacc
     1261 tgggttgtgg agtactcgcc ctggcttact ttcaggtggt gctgtgggac gtggcagccg
     1321 agagacagat tcacaaagtg aggcttcggt ttttccatgc catcctccgt caagagattg
     1381 cctggtttga tgtccataaa ggcggcgaac tcaacacaag gcttgcagat gacattgaca
     1441 agatccgcaa tggcattgga gacaagcttg gcatcatgct gcagtacacg gccacgttcg
     1501 ttgctggcat caccatcggc ttcgtgaaga gctggaagct cacccttgtc atcctggcag
     1561 tctctctgat actaattgta cccctggtgg gatctacatc cgtgattatc cagcggatga
     1621 ccaagcaagc cctggatgcc tatgctaagg caggggcgat cgcgggtgag gtcttctcag
     1681 gcataaggac tgtggtcgca ttcaacgggg aggagaagga gatggtcagg tactccagca
     1741 acttggacca agctaaatca aagacggtca aaaaggattt tgccacacta cttgcccagg
     1801 gcttcctttt cttctccatg ttctccagct atgccattgc cttctggtat ggcacagttc
     1861 tgtatttaga taatgaaatt acacctggag atatcctaac gacattcctt gctgtgctct
     1921 ttggtgcgtt tgccattggc caagcgggtc cgaactacag tgacttcaca acagctcggg
     1981 cggctgcatc tagcatctgg gaagtcatag accagatccc taccatcgac tgcttctcga
     2041 ccgacggaaa gaaggagaag attaccggac aggtgacctt tgaaggggtc catttctcgt
     2101 acccgtccag agcatcggtc aaggtgctca atgggatcaa cctgaaggtg gacgttggga
     2161 agacggtggc catggtcggc agcagtggat gcgggaagag cacctgcatc cagctcattc
     2221 agaggttcta tgatgttgct gaaggcagca tcaaaattga cggcattgac atccgtgacc
     2281 tcaacgtgag ctggcttcga gatcacatcg gtgtcgtgag ccaggaaccc atcctgtttg
     2341 ccaccacgat cgaggagaac atccgctatg gacgtcttga cgtcacccag gcagagattg
     2401 agaaagccgc cgaggaagcc aacgcccatg acttcatctc caaacttcca gagggttact
     2461 ccacattggt tggagagcga ggagctcagc tgtctggtgg tcagaagcag aggatagcca
     2521 ttgccagggc actggtcagg aaccctacaa tcctactcct ggacgaagcc acatcggctc
     2581 tagacacgga gagtgaggct accgttcaac tcgccctcga aaaggcccag cacggtcgaa
     2641 ccaccctcgt catcgctcac cgtctctcaa ccatcttcaa ctctgacctc atctgcgctt
     2701 tcaaggaggg cgtcatctcc gagcagggca cccatgaaga gctgatgaag aacgagggcg
     2761 gtgtttatca cacgctcgtc atgaagcagg gcatgaagaa agaggaggag gagaagaaag
     2821 agaacgaggt tccgctcgat gatgatgatg acgaagagga tgatagccaa ggtgagaagg
     2881 tctacagagc aggctctgga aagaagaagc tgacacgagt gctatcacgg acacagtcac
     2941 agatgtctgg agatgaagaa aaacaggatg aagacgagta cgagaaggag cttgagaagc
     3001 atttcagcat gatgcgagtc tggaagctga acaccccaga gtgtggtttc atcctcctgg
     3061 gatgcatcgg ggccgcaatc aatggggccg tccagcctgg attcgccgtc gtcttcagta
     3121 aaattcttgg ggcttacagc attacagata gagctgccct ctttgatgaa gtgactatat
     3181 actgtgttct gtttgctgcc cttggtctac tctccttact cgccagtatc atacagggtg
     3241 ttggatttgg caagtcggga ggtgagctga cactgaggct tcgtaacatg atgtttagag
     3301 ctatcctcag acagaacatt tctttctttg atgacaagag gaatggaacc ggtgccttga
     3361 caactaaatt ggcgacggat gtctctctta tacaaggggt gactggagta agacttggta
     3421 tgatctttga agtgctcttc aacatcggtg ttggtatcgt gatctcgttc gtctacagct
     3481 ggcagatcgc ttgtctcctt ctctttgcct tcctccccat cctgtccttg gccggtatga
     3541 ttggttggaa gatcctccag ggtaactcca tcggaacagc cggtagccag gctgaggttg
     3601 ggaagcttgt ctccgagtgt attgagaaca tacgtaccgt tcagtccctc aaccgaggac
     3661 agaccttcca tctgaagtac tgtgaactcc agaaccctcc gtacaaacaa gggatcaagg
     3721 gagctttcgc cgccggtctt gcgttcggct tctctcaagc taccatcttc tttgcatact
     3781 cagctacttt ccggcttggt gcccaccttg tgggtactgg agacctcacc ttccctgatg
     3841 ttttcctgtc tttctctgcc ttgatgtttg gtgcctttgg tctcggtcgc gctgcaggat
     3901 ctgtgccaga cttctccaag gctaaggtgg caacaggaga gctcttctat ttggtcgacc
     3961 gtagtccaga cattgacact ttcagcgatg acggggagaa accggcgagc tacggtggat
     4021 cggtgagcct caacaacgtt cggttccgct acccgacccg tcctgatgtc cctgtcctga
     4081 gggggctgtc cgtctcggtg gaccctggcg agaccctggc tttggtcggc agcagcggtt
     4141 gcggcaagag caccaccatc cagctgatgg agcgcttcta tgaccctcac tcagggactg
     4201 tgatgtttga ctcccacgat gcctcgctgc tcaacactcg ctggcagcgc gcccaggtgg
     4261 gcctcgtatc ccaagaaccc tgcctcttcg acatgtccat cgcagagaac atcaagtacg
     4321 gcgacaactc tcgggaagtc tccatcgaag actgcatcga agcggccaaa aagtccaaca
     4381 tccatgactt tgtggactct cttcccatga aatacgacac caacgttgga tccaagggca
     4441 cccagctgtc cggcggtcag aagcagagga tagccattgc cagggcactg gtcaggaacc
     4501 ccaaggtcct gctcctggat gaagccacat cggccttgga cactgaaagc gaaagggttg
     4561 tacaagatgc cctggatgaa gccaagaagg gccggacctg catcaccatc gcccatcgcc
     4621 tctcaaccat ccacaatgca gagaagatcg ctgtcatccg agaaggaaag ctcgccgagt
     4681 ttggcaagca tgaagagctg atggctatga agcagcaata ttattccctc tacactgccc
     4741 agtctatgca gcattaactc gagcctctag aactatagtg agtcgtatta cgtagatcca
     4801 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4861 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4921 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     4981 gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt
     5041 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5101 gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag
     5161 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5221 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5281 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5701 gttcggtgta
//
