LOCUS       sequence-429254-        9303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry-POLL in mammalian cells from a retroviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: mCherry-POLL
FEATURES             Location/Qualifiers
     source          1..9303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2901
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2339..2357)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2607..2626
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        4698..5197
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4733..4756)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5130..5149
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5218..5817
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5218..5237)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5714..5734
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6002..6434
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6537..6559)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6695..6715)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6700..7029
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6880..7015
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7051..7070
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7151..7168)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7322..7910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7402..7421)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8081..8872,8873..8941))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8704..8723
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8942..9046)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9114..9132
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9232..9303,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
     2221 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2281 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2341 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2401 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2461 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     2521 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2581 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2641 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     2701 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2761 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2821 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2881 ggcatggacg agctgtacaa gtccggactc agatctcgag gaggcggagg aagcgatccc
     2941 aggggtatct tgaaggcatt tcccaagcgg cagaaaattc atgctgatgc atcatcaaaa
     3001 gtacttgcaa agattcctag gagggaagag ggagaagaag cagaagagtg gctgagctcc
     3061 cttcgggccc atgttgtgcg cactggcatt ggacgagccc gggcagaact ctttgagaag
     3121 cagattgttc agcatggcgg ccagctatgc cctgcccagg gcccaggtgt cactcacatt
     3181 gtggtggatg aaggcatgga ctatgagcga gccctccgcc ttctcagact accccagctg
     3241 cccccgggtg ctcagctggt gaagtcagcc tggctgagct tgtgccttca ggagaggagg
     3301 ctggtggatg tagctggatt cagcatcttc atccccagta ggtacttgga ccatccacag
     3361 cccagcaagg cagagcagga tgcttctatt cctcctggca cccatgaggc cctgcttcag
     3421 acagcccttt ctcctcctcc tcctcccacc aggcctgtgt ctcctcccca aaaggcaaaa
     3481 gaggcaccaa acacccaagc ccagcccatc tctgatgatg aagccagtga tggggaagaa
     3541 acccaggtta gtgcagctga tctggaagcc ctcatcagtg gccactaccc cacctccctt
     3601 gagggagatt gtgagcctag cccagcccct gctgtcctgg ataagtgggt ctgtgcacag
     3661 ccctcaagcc agaaggcgac caatcacaac ctccatatca cagagaagct ggaagttctg
     3721 gccaaagcct acagtgttca gggagacaag tggagggccc tgggctatgc caaggccatc
     3781 aatgccctca agagcttcca taagcctgtc acctcgtacc agggggcctg cagtatccct
     3841 gggattggga agcggatggc tgagaaaatc atagagatcc tggagagcgg gcatttgcgg
     3901 aagctggacc atatcagtga gagcgtgcct gtcttggagc tcttctccaa catctgggga
     3961 gctgggacca agactgccca gatgtggtac caacagggct tccgaagtct ggaagacatc
     4021 cgcagccagg cctccctgac aacccagcag gccatcggcc tgaagcatta cagtgacttc
     4081 ctggaacgta tgcccaggga ggaggctaca gagattgagc agacagtcca gaaagcagcc
     4141 caggccttta actctgggct gctgtgtgtg gcatgtggtt cataccgacg gggaaaggcg
     4201 acctgtggtg atgtcgacgt gctcatcact cacccagatg gccggtccca ccggggtatc
     4261 ttcagccgcc tccttgacag tcttcggcag gaagggttcc tcacagatga cttggtgagc
     4321 caagaggaga atggtcagca acagaagtac ttgggggtgt gccggctccc agggccaggg
     4381 cggcggcacc ggcgcctgga catcatcgtg gtgccctata gcgagtttgc ctgtgccctg
     4441 ctctacttca ccggctctgc acacttcaac cgctccatgc gagccctggc caaaaccaag
     4501 ggcatgagtc tgtcagaaca tgccctcagc actgctgtgg tccggaacac ccatggctgc
     4561 aaggtggggc ctggccgagt gctgcccact cccactgaga aggatgtctt caggctctta
     4621 ggcctcccct accgagaacc tgctgagcgg gactggtgag gatccaccgg atctagataa
     4681 ctgatcataa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     4741 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     4801 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     4861 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     4921 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     4981 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     5041 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     5101 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     5161 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg
     5221 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     5281 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     5341 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     5401 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     5461 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     5521 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5581 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     5641 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5701 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5761 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     5821 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggcgtct
     5881 cgatcgagat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
     5941 ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg
     6001 gaatgaaaga ccccacctgt aggtttggca agctactagc ttaagtaacg ccattttgca
     6061 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     6121 gaacagggtc gatcgaccct agagaaccat cagatgtttc cagggtgccc caaggacctg
     6181 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     6241 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     6301 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     6361 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     6421 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
     6481 cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6541 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     6601 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     6661 tcacgtagcg atagcggagt gtagatccgg ctgtggaatg tgtgtcagtt agggtgtgga
     6721 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6781 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6841 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6901 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6961 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7021 ttttgcaaaa agcttactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     7081 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     7141 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7201 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7261 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7321 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7381 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7441 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7501 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7561 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7621 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7681 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7741 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7801 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7861 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7921 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7981 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8041 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8101 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8161 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8221 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8281 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8341 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     8401 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8461 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8521 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8581 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8641 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     8701 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8761 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8821 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8881 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8941 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9001 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9061 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     9121 gtatcacgag gccctttcgt cttcaagaat tagcttggcc attgcatacg ttgtatccat
     9181 atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
     9241 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     9301 agt
//
