LOCUS       sequence-429274-       10734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express a Mitophagy reporter in mammalian cells (untested).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10734)
  TITLE     Harper Lab plasmids
REFERENCE   2  (bases 1 to 10734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)
FEATURES             Location/Qualifiers
     source          1..10734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1847..1866)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             2852..3085
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3157..3745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3263..3274
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3672..3692
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3746..4144)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      4141..4150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4169..4380)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    4488..4512
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(4513..5226,5227..5229))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4558..4579)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4907..4926
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5166..5187
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(5236..6120)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     regulatory      6372..6381
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6384..6408)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6415..6914)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6463..6482)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6856..6879
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    7010..7127
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7425..7469)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7654..7887
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8384..8509
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8556..8736
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8754..8952)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8779..8799)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8954..9333
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9505..9524
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9599..9703
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9704..9772,9773..10564)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9922..9941)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctgctagaga ttttccacac tgactaaaag
     2881 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     2941 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     3001 caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt
     3061 cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct
     3121 acagctgcct tgtaagtcat tggtcttaaa gtcgacgcgg ggaggcggcc caaagggaga
     3181 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
     3241 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
     3301 gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca
     3361 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     3421 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
     3481 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     3541 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     3601 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     3661 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
     3721 aaattttgta atccagaggt tgattttagc cctcccacac ataaccagag ggcagcaatt
     3781 cacgaatccc aactgccgtc ggctgtccat cactgtcctt cactatggct ttgatcccag
     3841 gatgcagatc gagaagcacc tgtcggcacc gtccgcaggg gctcaagatg cccctgttct
     3901 catttccgat cgcgacgata caagtcaggt tgccagctgc cgcagcagca gcagtgccca
     3961 gcaccacgag ttctgcacaa ggtcccccag taaaatgata tacattgaca ccagtgaaga
     4021 tgcggccgtc gctagagaga gctgcgctgg cgacgctgta gtcttcagag atggggatgc
     4081 tgttgattgt agccgttgct ctttcaatga gggtggattc ttcttgagac aaaggcttgg
     4141 ccatggtggc agcgctctag aaccggtcct gtgttctggc ggcaaacccg ttgcgaaaaa
     4201 gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc
     4261 ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg
     4321 atcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc
     4381 actgacgggc accggagcca attcccactc ctttcaagac ctagctagtc ctgcaggttc
     4441 gctaccttag gaccgttata gttacgctcg aggtttaaac tcattaaacc actttgtaca
     4501 agaaagctgg gtttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
     4561 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gtttggactg
     4621 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
     4681 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
     4741 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
     4801 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
     4861 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
     4921 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
     4981 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
     5041 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
     5101 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
     5161 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
     5221 cttgctcacc tcgagaccgg aaatctccag agtagacagc cagcgcgcga tctcgctgcc
     5281 gatcaggtcc gggttgtctt cttgcagcag attcagacct gggccgatgt ccacagcctt
     5341 gcagttaggc aggcttttgg ccaggcgagc ggcttcggcc ggtgggatca gaacgcctgg
     5401 ggtgccccag aacagcagct tcgggacagg ggactggtgc agccagtcca tgtattcttc
     5461 gaccagcgcg acgatgttcg ctggctcacc ggcgattggc agctcgtttg ggaagcgcca
     5521 cagtggctcg cggtcaacag gattcaggaa cggctcgcgg taatggtcca tctcgacttc
     5581 agtcagcggg cggacgacac ccatcggcag cgtaccctcg ataaaaacgt tctgatcgat
     5641 gatcagcttg cggccgacgt cggtggtgcg gaaggcctgg aaggtctcgc gggcaaattc
     5701 tggccattcg tcccaggtcg ggatagggcg gatgaactcc ataaatgcaa tacctttgac
     5761 gcgctctgga ttgcgcttgg cccagtggaa acccagagcg gagccccagt cgtgaatgac
     5821 caggacgacc tcttccagac ccagggcttc gatgaaggca tccatgaagc ggacgtggtc
     5881 gtcgaagaaa taacccaggt ctggtttgtc ggatttgccc ataccgatca ggtctggagc
     5941 aatgcagcga tgggtcggtg caacatgcgg gatgatgttg cgccacacgt aggaggaggt
     6001 cgggttaccg tgcaggaaca gcacaggggt gccatcgcgc ggaccaacat cgacgtagtg
     6061 catgcgctcg cccaggactt ccacataatg ggggtcgaat ggaaagccag taccgatttc
     6121 gcctgaacca gagcccgaat tcccggatcc ggtggcgacc ggtggatcag aagagtaggc
     6181 gcgcttctgg aaagcctggc gggtggtggc gttgacgatg gaggtcatct gggtgcgctt
     6241 gagggtctgg aggggggagc cagtctggat ggtgcgcttg ctgacctgag caacgcggac
     6301 agcagggcgg gcaaccttgg cggaagcagc catctgggag gccaggcgag aggcgaggac
     6361 acgagtggag gccatggtgg cggagcctgc ttttttgtac aaacttgttt cgaacgaaag
     6421 gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa
     6481 tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc
     6541 gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta
     6601 ttggccgctg ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac
     6661 gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc
     6721 gggggggaac ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa
     6781 cggagccggt tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt
     6841 gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag
     6901 cgcctcccct acccataggg ataacagggt aatataggcg gccgcgtaga tggcgcgccg
     6961 gtaccttaat taaccaaact ggatctctgc tgtccctgta ataaacccga aaattttgaa
     7021 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
     7081 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata
     7141 ctgccatttg tctgcagaat tggcgcacgc agtgccgatc cgttcactaa tcgaatggat
     7201 ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc
     7261 ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact
     7321 atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat
     7381 aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta
     7441 tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag
     7501 gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga
     7561 ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg
     7621 caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt
     7681 tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga
     7741 tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat
     7801 aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct
     7861 cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact
     7921 cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta
     7981 tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc
     8041 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
     8101 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
     8161 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
     8221 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
     8281 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
     8341 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
     8401 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
     8461 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
     8521 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
     8581 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     8641 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     8701 ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaac gcgctgctta
     8761 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     8821 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     8881 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     8941 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     9001 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     9061 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     9121 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     9181 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     9241 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     9301 attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc
     9361 gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt
     9421 gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag
     9481 ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     9541 ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg
     9601 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9661 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9721 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9781 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9841 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9901 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     9961 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    10021 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    10081 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    10141 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    10201 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    10261 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    10321 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10381 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10441 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10501 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10561 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10621 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10681 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
